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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0088 [new locus tag: NWMN_RS00500 ]
  • pan locus tag?: SAUPAN000959000
  • symbol: NWMN_0088
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0088 [new locus tag: NWMN_RS00500 ]
  • symbol: NWMN_0088
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 111859..113400
  • length: 1542
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    GTGAGGATGTCAAACATAGCATTTTATGTCGTGAGTGACGTACATGGTTATATTTTCCCA
    ACAGATTTTACGAGTAGAAATCAATATCAACCTATGGGATTGTTACTAGCGAATCATGTT
    ATAGAACAAGACAGAAGGCAGTATGACCAAAGTTTTAAAATAGATAATGGTGATTTTTTG
    CAAGGGTCACCATTTTGTAATTACTTAATCGCGCATAGCGGCAGTAGCCAGCCTTTAGTT
    GATTTTTATAATCGAATGGCATTCGACTTTGGTACGCTTGGTAATCATGAATTTAATTAT
    GGATTACCATACTTAAAAGACACTTTACGCAGACTCAATTATCCAGTTTTGTGCGCTAAT
    ATATATGAAAATGATAGTACATTGACTGATAACGGTGTGAAGTATTTTCAGGTTGGAGAT
    CAAACTGTTGGTGTGATAGGTTTAACGACACAATTTATTCCCCATTGGGAACAACCAGAG
    CATATTCAGTCACTTACGTTTCATAGTGCTTTTGAAATACTTCAACAATACTTACCTGAA
    ATGAAGCGACATGCAGATATCATTGTGGTTTGTTACCATGGTGGATTTGAAAAGGATTTA
    GAAAGTGGTACGCCGACCGAAGTATTAACGGGTGAAAATGAAGGATATGCCATGTTAGAA
    GCGTTTTCTAAAGATATAGATATCTTTATTACGGGTCACCAACATCGACAAATTGCTGAA
    AGGTTTAAGCAAACGGCTGTGATTCAACCTGGTACGAGAGGTACAACTGTAGGCAGAGTA
    GTCTTGAGTACTGATGAATATGAAAATCTATCCGTTGAATCATGTGAATTACTTCCTGTT
    ATAGATGATTCCACATTTACTATTGATGAAGATGACCAACATTTACGAAAGCAGTTAGAG
    GACTGGTTAGATTACGAAATTACTACATTGCCATATGATATGACGATTAATCATGCATTT
    GAGGCACGTGTGGCACCGCATCCTTTTACAAATTTTATGAATTACGCTTTATTAGAAAAA
    AGTGACGCAGATGTTGCCTGTACAGCTTTGTTTGATTCTGCTAGTGGTTTCAAGCAAGTC
    GTGACGATGCGAGATGTTATTAACAATTACCCATTTCCAAATACATTTAAAGTTTTAGCT
    GTAAGTGGTGCCAAACTTAAAGAAGCCATTGAACGATCAGCAGAATATTTTGACGTGAAA
    AATGATGAAGTTAGTGTGAGCGCAGACTTCCTTGAACCCAAACCACAACACTTTAATTAT
    GATATATATGGTGGCGTAAGTTATACCATTCATGTTGGAAGACCAAAGGGACAACGTGTG
    AGCAATATGATGATTCAAGGTCACGCAGTTGATTTAAAGCAGACATATACAATTTGTGTA
    AATAATTATCGTGCAGTAGGCGGTGGTCAGTATGATATGTATATCGACGCGCCAGTTGTA
    AAAGATATTCAAGTTGAAGGCGCACAATTACTTATTGATTTTTTATCAAATAATAATTTG
    ATGCGCATCCCGCAAGTTGTTGATTTTAAAGTTGAAAAGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1542

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0088 [new locus tag: NWMN_RS00500 ]
  • symbol: NWMN_0088
  • description: hypothetical protein
  • length: 513
  • theoretical pI: 4.66374
  • theoretical MW: 58397.2
  • GRAVY: -0.301754

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesOther2',3'-cyclic-nucleotide 2'-phosphodiesterase (TIGR01390; EC 3.1.4.16; HMM-score: 170.4)
    thiosulfohydrolase SoxB (TIGR04486; HMM-score: 83.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPyridine nucleotidesNAD nucleotidase (TIGR01530; EC 3.1.3.5,3.6.1.22; HMM-score: 56.5)
    MetabolismTransport and binding proteinsOtherNAD nucleotidase (TIGR01530; EC 3.1.3.5,3.6.1.22; HMM-score: 56.5)
    Unknown functionEnzymes of unknown specificityphosphodiesterase, MJ0936 family (TIGR00040; EC 3.1.-.-; HMM-score: 17.1)
  • TheSEED:  
    Nucleosides and NucleotidesPurinesPurine conversions 2',3'-cyclic-nucleotide 2'-phosphodiesterase (EC 3.1.4.16) 
    Pyrimidinespyrimidine conversions 2',3'-cyclic-nucleotide 2'-phosphodiesterase (EC 3.1.4.16) 
    Potassium metabolismPotassium metabolism - no subcategoryGlutathione-regulated potassium-efflux system and associated functions 2',3'-cyclic-nucleotide 2'-phosphodiesterase (EC 3.1.4.16) 
  • PFAM:
    no clan defined5_nucleotid_C; 5'-nucleotidase, C-terminal domain (PF02872; HMM-score: 98.1)
    Calcineurin (CL0163) Metallophos; Calcineurin-like phosphoesterase (PF00149; HMM-score: 30)
    Metallophos_2; Calcineurin-like phosphoesterase superfamily domain (PF12850; HMM-score: 14.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0.11
    • Cytoplasmic Membrane Score: 0.02
    • Cellwall Score: 8.97
    • Extracellular Score: 0.9
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.119
    • Ymax_pos: 17
    • Cmax: 0.121
    • Cmax_pos: 17
    • Smax: 0.145
    • Smax_pos: 43
    • Smean: 0.12
    • D: 0.119
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MRMSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRRQYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDFYNRMAFDFGTLGNHEFNYGLPYLKDTLRRLNYPVLCANIYENDSTLTDNGVKYFQVGDQTVGVIGLTTQFIPHWEQPEHIQSLTFHSAFEILQQYLPEMKRHADIIVVCYHGGFEKDLESGTPTEVLTGENEGYAMLEAFSKDIDIFITGHQHRQIAERFKQTAVIQPGTRGTTVGRVVLSTDEYENLSVESCELLPVIDDSTFTIDEDDQHLRKQLEDWLDYEITTLPYDMTINHAFEARVAPHPFTNFMNYALLEKSDADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIERSAEYFDVKNDEVSVSADFLEPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDLKQTYTICVNNYRAVGGGQYDMYIDAPVVKDIQVEGAQLLIDFLSNNNLMRIPQVVDFKVEK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]