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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0117 [new locus tag: NWMN_RS00650 ]
  • pan locus tag?: SAUPAN001011000
  • symbol: NWMN_0117
  • pan gene symbol?: ssuA
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0117 [new locus tag: NWMN_RS00650 ]
  • symbol: NWMN_0117
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 142389..143363
  • length: 975
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAAGGTTAAGCATAATCGTCATCATTGGAATCTTTATAATTACAGGATGTGATTGG
    CAAAGGACGTCTAAAGAACGGTCTAAAAATGCCCAAAATCAGCAAGTGATTAAAATTGGA
    TATTTGCCGATTACACATTCAGCTAATTTGATGATGACTAAAAAATTATTATCACAATAC
    AATCATCCGAAATATAAACTAGAATTAGTTAAATTCAATAATTGGCCAGATTTAATGGAC
    GCATTAAACAGTGGTCGTATTGATGGTGCATCAACTTTAATAGAGCTAGCGATGAAATCA
    AAACAGAAGGGCTCAAATATAAAGGCTGTGGCATTGGGCCATCATGAAGGCAATGTCATT
    ATGGGACAAAAAGGTATGCACTTAAATGAATTTAATAATAATGGCGATGATTACCATTTT
    GGTATACCACATCGTTATTCAACACATTATCTTTTACTTGAGGAATTACGTAAACAATTA
    AAGATTAAACCGGGGCATTTTAGCTATCATGAAATGTCGCCAGCAGAAATGCCAGCCGCA
    TTGAGTGAACACAGAATTACAGGGTATTCTGTAGCCGAACCATTCGGTGCACTGGGTGAA
    AAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGC
    TGTGTGCTAGTACTGAGAGGGGAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTT
    GTACAAGATTATAAAAAGTCTGGCTTTAAAATGAATGATCGCAAGCAAAGTGTAGACATT
    ATGACGCATCATTTTAAACAAAGTCGTGACGTTTTAACACAGTCAGCGGCATGGACATCC
    TATGGTGATTTAACAATTAAGCCATCCGGCTATCAAGAAATTACGACATTGGTAAAACAA
    CATCATTTGTTTAATCCACCTGCATATGATGACTTTGTTGAACCGTCATTGTATAAGGAG
    GCATCGCGTTCATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    975

Protein[edit source | edit]

Protein Data Bank: 3UN6

General[edit source | edit]

  • locus tag: NWMN_0117 [new locus tag: NWMN_RS00650 ]
  • symbol: NWMN_0117
  • description: hypothetical protein
  • length: 324
  • theoretical pI: 9.40092
  • theoretical MW: 36740.9
  • GRAVY: -0.490123

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsOtherABC transporter, substrate-binding protein, aliphatic sulfonates family (TIGR01728; HMM-score: 77.6)
    taurine ABC transporter, periplasmic binding protein (TIGR01729; HMM-score: 22.4)
    MetabolismTransport and binding proteinsAnionsphosphate/phosphite/phosphonate ABC transporter, periplasmic binding protein (TIGR01098; HMM-score: 14.4)
  • TheSEED:  
    FIG01108009: hypothetical protein 
  • PFAM:
    PBP (CL0177) NMT1_2; NMT1-like family (PF13379; HMM-score: 82.4)
    OpuAC; Substrate binding domain of ABC-type glycine betaine transport system (PF04069; HMM-score: 21.5)
    Lipoprotein_9; NLPA lipoprotein (PF03180; HMM-score: 16.3)
    SBP_bac_3; Bacterial extracellular solute-binding proteins, family 3 (PF00497; HMM-score: 15.4)
    no clan definedWBP-1; WW domain-binding protein 1 (PF11669; HMM-score: 14.7)
    PBP (CL0177) NMT1; NMT1/THI5 like (PF09084; HMM-score: 12.9)
    no clan definedDUF3290; Protein of unknown function (DUF3290) (PF11694; HMM-score: 12.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 0.67
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.296
    • Ymax_pos: 23
    • Cmax: 0.201
    • Cmax_pos: 23
    • Smax: 0.616
    • Smax_pos: 18
    • Smean: 0.387
    • D: 0.331
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKRLSIIVIIGIFIITGCDWQRTSKERSKNAQNQQVIKIGYLPITHSANLMMTKKLLSQYNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAMKSKQKGSNIKAVALGHHEGNVIMGQKGMHLNEFNNNGDDYHFGIPHRYSTHYLLLEELRKQLKIKPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDVIPDAYCCVLVLRGELLDQHKDVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVLTQSAAWTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: NWMN_1528 (CymR*)
    CymR* (TF) important in Cysteine metabolism  RegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]