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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0127 [new locus tag: NWMN_RS00700 ]
  • pan locus tag?: SAUPAN001023000
  • symbol: argJ
  • pan gene symbol?: argJ
  • synonym:
  • product: bifunctional ornithine acetyltransferase/N-acetylglutamate synthase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0127 [new locus tag: NWMN_RS00700 ]
  • symbol: argJ
  • product: bifunctional ornithine acetyltransferase/N-acetylglutamate synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 158711..159952
  • length: 1242
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAAACATCAAGAAACGACATCACAACAATATAACTTTTCAATTATTAAACATGGCGAT
    ATCAGTACACCTCAAGGCTTCACGGCTGGTGGTATGCACATCGGTTTACGCGCTAACAAA
    AAAGACTTTGGGTGGATTTACTCATCGTCTTTGGCAAGTGCAGCTGCCGTATATACTTTA
    AATCAGTTTAAAGCTGCACCACTTATTGTCACTGAAGACACTTTACAAAAGTCTAAAGGA
    AAATTACAAGCACTTGTTGTTAATTCAGCTAATGCAAATTCTTGTACCGGTCAACAAGGC
    ATAGATGATGCACGACAAACACAAACATGGGTTGCTCAACAACTTCAAATACCATCTGAG
    CATGTTGCTGTTGCTTCAACTGGGGTCATTGGTGAATATTTGCCTATGGATAAAATTAAG
    ACTGGGACCGAACATATTAAGGATGCTAATTTTGCAACGCCAGGTGCGTTTAACGAGGCA
    ATTTTAACAACTGATACCTGTACAAAACATATCGCTGTATCACTAAAAATCGATGGTAAA
    ACCGTTACAATTGGTGGTAGCACCAAAGGTTCAGGTATGATTCACCCAAATATGGCTACC
    ATGCTTGCTTTTATAACAACCGATGCATCGATTGAATCGAATACACTTCATCAATTATTA
    AAATCTTCGACTGACCATACATTTAATATGATTACTGTTGATGGCGATACAAGTACAAAT
    GACATGGTATTAGTCATGGCAAATCACCAAGTTGAACACCAAATACTTAGTCAAGACCAT
    CCACAATGGGAAACATTTGTTGATGCATTCAATTTTGTCTGTACATTTTTAGCTAAAGCT
    ATAGCCAGAGATGGCGAAGGCGCAACAAAGTTAATCAGCGTCAATGTGTCAGGCGCTAAA
    TCAATATCAGATGCAAGAAAAATCGGTAAAACTATCGTAAGTTCAAATCTAGTAAAATCA
    GCTATTTTTGGCGAAGATGCCAATTTTGGTCGAATCATTACAGCTATTGGCTACAGCGGA
    TGTGAAATTGATCCTAACTGCACATATGTTCAACTGAACCAAATACCTGTCGTTGATAAA
    GGTATGGCTGTACTATTTGATGAGCAAGCTATGTCGAATACATTAACTCATGAAAATGTC
    ACAATTGACGTTCAGCTTGGTTTAGGTAACGCTGCAGCGACTGCATACGGTTGTGATTTA
    TCCTATGATTATGTGCGTATCAACGCATCATATCGAACATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1242

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0127 [new locus tag: NWMN_RS00700 ]
  • symbol: ArgJ
  • description: bifunctional ornithine acetyltransferase/N-acetylglutamate synthase
  • length: 413
  • theoretical pI: 6.18088
  • theoretical MW: 44399.8
  • GRAVY: -0.108232

Function[edit source | edit]

  • reaction:
    EC 2.3.1.35?  ExPASy
    Glutamate N-acetyltransferaseN2-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate
    EC 2.3.1.1?  ExPASy
    Amino-acid N-acetyltransferaseAcetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate
  • TIGRFAM:
    MetabolismAmino acid biosynthesisGlutamate familyglutamate N-acetyltransferase/amino-acid acetyltransferase (TIGR00120; EC 2.3.1.1,2.3.1.35; HMM-score: 494.5)
  • TheSEED:  
    Arginine Biosynthesis extended Glutamate N-acetyltransferase (EC 2.3.1.35) / N-acetylglutamate synthase (EC 2.3.1.1) 
  • PFAM:
    DmpA_ArgJ (CL0635) ArgJ; ArgJ family (PF01960; HMM-score: 481.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 0.67
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.173
    • Ymax_pos: 66
    • Cmax: 0.229
    • Cmax_pos: 66
    • Smax: 0.194
    • Smax_pos: 63
    • Smean: 0.126
    • D: 0.154
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFGWIYSSSLASAAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGIDDARQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHIKDANFATPGAFNEAILTTDTCTKHIAVSLKIDGKTVTIGGSTKGSGMIHPNMATMLAFITTDASIESNTLHQLLKSSTDHTFNMITVDGDTSTNDMVLVMANHQVEHQILSQDHPQWETFVDAFNFVCTFLAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIITAIGYSGCEIDPNCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHENVTIDVQLGLGNAAATAYGCDLSYDYVRINASYRT

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: AhrC (ArgR*)
    AhrC  (ArgR*) (TF) important in Arginine biosynthesis, Arginine degradationRegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]