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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0177 [new locus tag: NWMN_RS00990 ]
  • pan locus tag?: SAUPAN001110000
  • symbol: NWMN_0177
  • pan gene symbol?: glcC
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0177 [new locus tag: NWMN_RS00990 ]
  • symbol: NWMN_0177
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 229587..231122
  • length: 1536
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGTTGATGAAATCTTTATTTGAAAAAGCACAGCAGTTCGGCAAGTCCTTTATGTTACCT
    ATCGCAATCTTACCAGCTGCAGGTCTATTGTTGGGTATCGGTGGTGCATTAAGTAATCCA
    AACACCGTTAAAGCATACCCTATTTTAGATATTACCTTATTACAAAATATTTTTACATTA
    ATGTCAGCTGCAGGTAGTATTGTTTTCCAAAATTTACCGGTCATCTTTGCAATTGGTGTC
    GCAATCGGATTATCTAGAAGCGATAAAGGTACTGCAGGTTTAGCTGCGCTGCTCGGTTTC
    TTAATTATGAACGCAACTATGAATGGCTTATTAACTATCACGGGCACATTGGCAAAAGAT
    CAGCTTGCACAAAATGGACAAGGCATGGTGCTCGGTATACAAACGGTTGAAACCGGTGTT
    TTTGGCGGGATTATCACAGGTATTATGACCGCAATACTTCACAACAAATATCACAAAGTG
    GTATTACCACCGTATTTAGGTTTCTTTGGTGGCTCTAGATTTGTCCCTATTGTCACAGCA
    TTTGCCGCAATCTTTTTAGGTGTATTGATGTTTTTCATTTGGCCAAGCATACAAGCCGGC
    ATTTATCATGTTGGTGGATTTGTAACGAAAACAGGTGCCATCGGTACTTTTGTTTATGGC
    TTCATCTTAAGATTGTTAGGTCCACTCGGTTTACACCATATTTTTTACTTACCGTTTTGG
    CAGACGGCACTTGGTGGTACTTTAGAAGTCAAAGGGCACTTAGTTCAAGGTACGCAGAAC
    ATCTTCTTTGCTCAACTTGGTGATCCAGATGTGACGAAGTATTATTCAGGTGTGTCACGC
    TTTATGTCAGGCCGTTTTATTACGATGATGTTCGGCTTATGTGGTGCCGCACTTGCAATT
    TATCACACAGCTAAACCTGAACATAAAAAAGTTGTCGGCGGTTTAATGTTATCCGCTGCA
    CTCACTTCATTTTTAACAGGTATTACCGAACCTTTAGAGTTTAGTTTCTTGTTTGTCGCA
    CCTATTCTTTATGTAATCCATGCCTTCTTTGATGGATTAGCATTTATGATGGCAGACATT
    TTCAACATTACAATTGGTCAAACCTTCAGTGGAGGCTTTATCGATTTCTTACTCTTTGGT
    GTGCTACAAGGTAATAGTAAAACAAACTACCTATACGTCATACCTATTGGAATTGTGTGG
    TTCTGTTTGTATTACATCGTTTTCAGATTCTTAATTACGAAATTTAATTTCAAAACACCT
    GGTCGAGAAGATAAAGCTGCAGCACAACAAGTTGAGGCTACTGAAAGAGCACAAACTATT
    GTTGCTGGTTTGGGAGGCAAAGATAACATTGAAATCGTTGACTGTTGTGCAACGAGACTA
    CGCGTCACACTTCATCAAAATGACAAAGTCGATAAAGTATTACTCGAAAGTACTGGTGCC
    AAAGGTGTAATCCAGCAAGGCACTGGTGTGCAAGTAATTTATGGGCCTCACGTTACAGTT
    ATCAAAAATGAAATTGAAGAATTGCTCGGGGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1536

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0177 [new locus tag: NWMN_RS00990 ]
  • symbol: NWMN_0177
  • description: hypothetical protein
  • length: 511
  • theoretical pI: 8.96532
  • theoretical MW: 55177.7
  • GRAVY: 0.622309

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, glucose-specific IIBC component (TIGR02002; EC 2.7.1.69; HMM-score: 591.6)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, maltose and glucose-specific IIBC component (TIGR02004; EC 2.7.1.69; HMM-score: 463.4)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, N-acetylglucosamine-specific IIBC component (TIGR01998; EC 2.7.1.69; HMM-score: 450.2)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, alpha-glucoside-specific IIBC component (TIGR02005; EC 2.7.1.69; HMM-score: 314.1)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 285.1)
    Signal transductionSignal transductionPTSPTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 285.1)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, IIBC component (TIGR02003; EC 2.7.1.69; HMM-score: 268.6)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 73.4)
    Signal transductionSignal transductionPTSPTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 73.4)
    PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 70)
    PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 44.1)
    PTS system, trehalose-specific IIBC component (TIGR01992; EC 2.7.1.69; HMM-score: 43.5)
  • TheSEED:  
    Maltose and Maltodextrin Utilization PTS system, N-acetylglucosamine-specific IIA component / PTS system, N-acetylglucosamine-specific IIB component (EC 2.7.1.69) / PTS system, N-acetylglucosamine-specific IIC component 
  • PFAM:
    PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 311.9)
    no clan definedPTS_EIIB; phosphotransferase system, EIIB (PF00367; HMM-score: 49.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.324
    • Ymax_pos: 46
    • Cmax: 0.656
    • Cmax_pos: 46
    • Smax: 0.447
    • Smax_pos: 10
    • Smean: 0.257
    • D: 0.298
  • predicted transmembrane helices (TMHMM): 11

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLMKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAYPILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMNATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVLPPYLGFFGGSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYGFILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGVSRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSFLFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVIPIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGKDNIEIVDCCATRLRVTLHQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLGD

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_0177 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator: NWMN_0259 (NanR*)
    NanR* (TF) important in N-acetylglucosamine utilization, Sialic acid utilizationRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]