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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0205 [new locus tag: NWMN_RS01150 ]
  • pan locus tag?: SAUPAN001157000
  • symbol: rbsR
  • pan gene symbol?: rbsR
  • synonym:
  • product: ribose transcriptional repressor RbsR

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0205 [new locus tag: NWMN_RS01150 ]
  • symbol: rbsR
  • product: ribose transcriptional repressor RbsR
  • replicon: chromosome
  • strand: -
  • coordinates: 262359..263357
  • length: 999
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAAAAGTGTCAATTAAAGATGTTGCTAGAGAAGCTGGTGTATCAGTTACAACTGTG
    TCACATATTTTAAATCATAATGATAGTCGTTTTTCCGCAACAACGATAAAAAACGTACAT
    GCTGTTTCAGAACGTTTAGGCTATGCCCCTAATAAACATGCAAAACAATTGCGCGGCAGT
    AAAATTCAAACTATTGGCGTCATTTTGCCTAGCTTAACAAATCCGTTTTTCTCAGCACTG
    ATGCAAAGTATTCATGACCATAAACCATCTGATGTTGATTTATGCTTTTTAACATCTACA
    GCAACTGATTTGTATGACAATATTAAACATTTAATTGATCGAGGTATTGACGGATTAATT
    ATCGCACAATACATATCATCCCCGGACGCCCTAAATAACTATCTAAAGAAACATCATGTA
    CCTTATGTCGTACTGGATCAAAATGACCATCAAGGCTATACAGATTTTGTTCGGACAAAT
    GAATATCAAGGTGGACAACTTGCAGCACAACATTTAATAGAACTCGGTCACAACCATATG
    ATAATTGTTGCACCATATGACATGATGGCGAATATGTCGACTCGTGTCGCTGGATTTGTC
    GATACTTTGCGCGCGAATCAATTGCCAGAACCACAAATCGTCCATACTGAATTATCTAAG
    CGCGGTGGGCTAACCATTGTTGATGACATCATGGTTCAATCTGCCACTGCAATCTTCGCT
    ATTAACGATGAACTCGCTATTGGCATTTTACGAGGACTAATTGAACATGGCATCAGTATC
    CCGAAAGATATCTCATTAATAGGTTATGACGACATTGATTATGCAGCGTACGTCTCGCCA
    CCTTTAACTACTGTGGCACAACCTATAACTGATATTGGCAAAACATCTTTAACCTTATTA
    CTTCAACGATTACAGCACTTAGATAAATCCATTGATATGATTGAATTACCAACGACTTTA
    AAAATTCGTGCAACAACTGGCTATCATCTTTCAAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    999

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0205 [new locus tag: NWMN_RS01150 ]
  • symbol: RbsR
  • description: ribose transcriptional repressor RbsR
  • length: 332
  • theoretical pI: 6.76486
  • theoretical MW: 36657.7
  • GRAVY: -0.0650602

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Signal transductionRegulatory functionsDNA interactionscatabolite control protein A (TIGR01481; HMM-score: 172.7)
    Signal transductionRegulatory functionsDNA interactionsD-fructose-responsive transcription factor (TIGR02417; HMM-score: 117.9)
    Signal transductionRegulatory functionsDNA interactionstrehalose operon repressor (TIGR02405; HMM-score: 74.3)
    Signal transductionRegulatory functionsDNA interactionstranscriptional repressor BetI (TIGR03384; HMM-score: 13.9)
    Genetic information processingProtein synthesistRNA aminoacylationaspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit (TIGR00132; EC 6.3.5.-; HMM-score: 11)
  • TheSEED:  
    CarbohydratesMonosaccharidesD-ribose utilization Ribose operon repressor 
  • PFAM:
    Periplas_BP (CL0144) Peripla_BP_3; Periplasmic binding protein-like domain (PF13377; HMM-score: 122.2)
    Peripla_BP_1; Periplasmic binding proteins and sugar binding domain of LacI family (PF00532; HMM-score: 82.2)
    Peripla_BP_4; Periplasmic binding protein domain (PF13407; HMM-score: 61.8)
    HTH (CL0123) LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 57.3)
    HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 20)
    HTH_3; Helix-turn-helix (PF01381; HMM-score: 18.8)
    HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 15.3)
    HTH_23; Homeodomain-like domain (PF13384; HMM-score: 14.6)
    HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 14)
    TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 13.5)
    HTH_19; Helix-turn-helix domain (PF12844; HMM-score: 13.4)
    HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 13.4)
    CENP-B_N; CENP-B N-terminal DNA-binding domain (PF04218; HMM-score: 13.1)
    IF2_N; Translation initiation factor IF-2, N-terminal region (PF04760; HMM-score: 13)
    HTH_10; HTH DNA binding domain (PF04967; HMM-score: 12.7)
    no clan definedPucR; Purine catabolism regulatory protein-like family (PF07905; HMM-score: 12.6)
    TnsD; Tn7-like transposition protein D (PF15978; HMM-score: 10.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.224
    • Ymax_pos: 33
    • Cmax: 0.222
    • Cmax_pos: 33
    • Smax: 0.439
    • Smax_pos: 3
    • Smean: 0.274
    • D: 0.243
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSERLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTATDLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTNEYQGGQLAAQHLIELGHNHMIIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTELSKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAAYVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: SigB [1] other strains
    SigB (sigma factor) controls a large regulon involved in stress/starvation response and adaptation
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J. Bacteriol.: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

Relevant publications[edit source | edit]