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04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0263 [new locus tag: NWMN_RS01450 ]
  • pan locus tag?:
  • symbol: int
  • pan gene symbol?:
  • synonym:
  • product: integrase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0263 [new locus tag: NWMN_RS01450 ]
  • symbol: int
  • product: integrase
  • replicon: chromosome
  • strand: -
  • coordinates: 320846..322051
  • length: 1206
  • essential: unknown

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGTGGTTTGAAAAATTTAAAAATAAGAACAATGAAACGAAGTATAGATACTACGAGAAA
    TACAAAGATCCGTATACAGATAAATGGAAACGTGTAAGTGTTGTCTTGAATAAGAATACA
    AAGCAATCGCAAAAAGAGGCAATGTTTCGTTTAGAAGAAAAAATAAAAGAAAAACTAAAC
    AACAAGTCGTCAAGCGAATTAAAAACTTTGACTTTTCACGCGTTATTAGATGAATGGCTT
    GAATATCATATAAAAACATCTGGCTTTAAAGTAACGACGCTTGATAATTTGAAAACAAGA
    ATCAAAAACATCAAAAAGAACAGTTCTCAAAATTTACTTTTAAACAAAATTGATACAAAG
    TACATGCAAACATTTATTAACGAATTATCAAACGTATATTCTGCAAATCAGGTAAAGCGT
    CAACTTGGACATATGAAAGAAGCTATTAAATACGCCGTTAAATTTTACAATTATCCAAAC
    GAACACATATTAAATAGCGTCACACTACCAAAGAAGAGTAAGACGATAGAAGATATAGAA
    AAAGAAGAAGCGAAAATGTACAACTATTTAGAGATGGAACAGGTAATACAGATACGCGAT
    TTTATACTGAACGATAATAACATGCAGTATAGAGCTCGTATTTTAGTTGCTGGGGCTGTA
    GAAGTTCAAGCTTTAACAGGTATGCGCATAGGTGAGTTATTAGCTCTCCAAGTTAAAGAT
    GTTGACCTCAAAAATAAAACGATCGCTATTAATGGCACTATTCACAGAATCAAATGTAAT
    GCTGGATTTGGTCACAAAGATACTACGAAGACCGCAGGTTCAAAAAGAAAAATCGCCATC
    AATTCAAGGATAGCAAATGTATTGAAAAAAATAATGTTAGAAAATAAAAAGATGCAACAA
    TGGGAACCAAGCTATGTTGATAGAGGGTTTATATTCACAACTTGCCAAGGAAATCCTATG
    CAAGGCAGTAGGATAAACAAACGATTGTCCTCAGCTGCAGAATCATTAAATATAAATAAA
    AAAGTTACTACTCACACACTAAGGCATACACACATAAGTTTATTGGCGGAAATGAATATA
    TCGTTAAAAGCAATTATGAAAAGAGTAGGACATAGAGATGAAAAAACGACTATAAAGGTG
    TATACACATGTAACAGAGAAAATGGACAGAGAGTTAGAGCAAAAATTAGAAAAACTTGTG
    TACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1206

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0263 [new locus tag: NWMN_RS01450 ]
  • symbol: Int
  • description: integrase
  • length: 401
  • theoretical pI: 10.3973
  • theoretical MW: 46984.2
  • GRAVY: -0.694015

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairtyrosine recombinase XerC (TIGR02224; HMM-score: 74)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairtyrosine recombinase XerD (TIGR02225; HMM-score: 70.6)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairintegron integrase (TIGR02249; HMM-score: 54.6)
    Genetic information processingMobile and extrachromosomal element functionsOtherintegron integrase (TIGR02249; HMM-score: 54.6)
  • TheSEED:  
    Phage integrase 
  • PFAM:
    DNA-mend (CL0382) Phage_integrase; Phage integrase family (PF00589; HMM-score: 100.1)
    l-integrase_N (CL0469) Phage_int_SAM_3; Phage integrase, N-terminal SAM-like domain (PF14659; HMM-score: 23.5)
    HIT (CL0265) DcpS_C; Scavenger mRNA decapping enzyme C-term binding (PF11969; HMM-score: 16.2)
    l-integrase_N (CL0469) Phage_int_SAM_1; Phage integrase, N-terminal SAM-like domain (PF02899; HMM-score: 14.5)
    GHD (CL0369) DUF3372; Domain of unknown function (DUF3372) (PF11852; HMM-score: 7.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.133
    • Ymax_pos: 54
    • Cmax: 0.105
    • Cmax_pos: 48
    • Smax: 0.251
    • Smax_pos: 46
    • Smean: 0.124
    • D: 0.129
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MWFEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQKEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGFKVTTLDNLKTRIKNIKKNSSQNLLLNKIDTKYMQTFINELSNVYSANQVKRQLGHMKEAIKYAVKFYNYPNEHILNSVTLPKKSKTIEDIEKEEAKMYNYLEMEQVIQIRDFILNDNNMQYRARILVAGAVEVQALTGMRIGELLALQVKDVDLKNKTIAINGTIHRIKCNAGFGHKDTTKTAGSKRKIAINSRIANVLKKIMLENKKMQQWEPSYVDRGFIFTTCQGNPMQGSRINKRLSSAAESLNINKKVTTHTLRHTHISLLAEMNISLKAIMKRVGHRDEKTTIKVYTHVTEKMDRELEQKLEKLVY

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]