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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0624 [new locus tag: NWMN_RS03545 ]
  • pan locus tag?: SAUPAN002533000
  • symbol: lipA
  • pan gene symbol?:
  • synonym:
  • product: lipase/esterase LipA

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0624 [new locus tag: NWMN_RS03545 ]
  • symbol: lipA
  • product: lipase/esterase LipA
  • replicon: chromosome
  • strand: +
  • coordinates: 705809..706852
  • length: 1044
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAATAAAGATAATAAATGGACGATGATAACTGCGCTTTTTATAACTGTAATCAGTGTA
    TTGTTAGCATTTCATCTGAAACAACATTATGACCAAATTACAAATGAGAACCATGCTAAT
    AAAGACAAAATTAATATTAAAAATAAAAATGTGCGCATTTATCAAAACCTTACATACAAT
    AGAGTTTTCCCTAACAGTAAATTAGATATTATTACACCTGTTGATATGTCTTCTAATGCC
    AAACTGCCAGTTATTTTTTGGATGCACGGTGGTGGTTATATTGCGGGTGATAAGCAGTAT
    AAAAACCCATTATTAGCGAAAATTGCTGAACAAGGGTACATTGTTGTGAATGTAAATTAT
    GCATTGGCGCCACAATATAAATATCCCACACCATTAATTCAAATGAATCAAGCAACTCAA
    TTCATTAAAGAAAATAAAATGAATTTACCTATTGATTTTAATCAAGTAATTATTGGCGGT
    GATTCTGCAGGTGCTCAATTAGCTAGCCAATTTACGGCAATACAGACGAATGATCGCTTA
    AGAGAAGCCATGAAATTTGATCAGTCATTCAAACCATCGCAAATTAAAGGTGCTATACTA
    TTTGGTGGTTTTTATAATATGCAAACAGTTAGAGAAACTGAGTTTCCAAGAATACAGTTA
    TTTATGAAAAGTTATACTGGCGAAGAAGATTGGGAAAAGAGTTTTAAAAACATTTCACAA
    ATGTCGACAGTAAAACAATCGACAAAAAATTATCCACCAACATTTTTATCTGTTGGAGAT
    AGCGATCCATTCGAAAGTCAAAATATAGAATTCAGTAAGAAATTACAAGAATTGAATGTA
    CCAGTAGATACTTTGTTTTATGATGGTACGCATCATTTACATCATCAGTATCAATTTCAC
    CTTAATAAACCTGAATCGATAGATAATATCAAAAAAGTGTTACTTTTCTTAAGTCGTAAT
    ACATCCTCTAGTGGTATTCAAACTGAAGAGAAACCACAAATAGAAAATCCGAGTAATGAA
    TTACCGTTAAATCCTTTAAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1044

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0624 [new locus tag: NWMN_RS03545 ]
  • symbol: LipA
  • description: lipase/esterase LipA
  • length: 347
  • theoretical pI: 9.18823
  • theoretical MW: 39838.1
  • GRAVY: -0.503458

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismFatty acid and phospholipid metabolismDegradationesterase, PHB depolymerase family (TIGR01840; EC 3.1.1.-; HMM-score: 11.5)
  • TheSEED:  
    Lipase LipA 
  • PFAM:
    AB_hydrolase (CL0028) Abhydrolase_3; alpha/beta hydrolase fold (PF07859; HMM-score: 110.8)
    COesterase; Carboxylesterase family (PF00135; HMM-score: 37.8)
    Peptidase_S9; Prolyl oligopeptidase family (PF00326; HMM-score: 25.7)
    Chlorophyllase2; Chlorophyllase enzyme (PF12740; HMM-score: 22.5)
    Say1_Mug180; Steryl acetyl hydrolase (PF10340; HMM-score: 17.2)
    Cutinase; Cutinase (PF01083; HMM-score: 15.3)
    Abhydrolase_2; Phospholipase/Carboxylesterase (PF02230; HMM-score: 13.6)
    PAF-AH_p_II; Platelet-activating factor acetylhydrolase, isoform II (PF03403; HMM-score: 13.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.3
    • Cellwall Score: 4.55
    • Extracellular Score: 5.15
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: 7
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.306
    • Ymax_pos: 31
    • Cmax: 0.148
    • Cmax_pos: 31
    • Smax: 0.837
    • Smax_pos: 25
    • Smean: 0.479
    • D: 0.373
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNKDNKWTMITALFITVISVLLAFHLKQHYDQITNENHANKDKINIKNKNVRIYQNLTYNRVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKNPLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGGDSAGAQLASQFTAIQTNDRLREAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRIQLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQELNVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQIENPSNELPLNPLN

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]