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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0813 [new locus tag: NWMN_RS04600 ]
  • pan locus tag?: SAUPAN003043000
  • symbol: NWMN_0813
  • pan gene symbol?:
  • synonym:
  • product: Na+/H+ antiporter family protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0813 [new locus tag: NWMN_RS04600 ]
  • symbol: NWMN_0813
  • product: Na+/H+ antiporter family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 901809..903125
  • length: 1317
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGATAAATGCAGTAGTAATAGCAGTAATTTTAATGATTGTGCTATGTTTATGTCGATTA
    AACGTAGTTATAAGCTTATTTATCAGTGCGCTAGTTGGTGGCTTAATTTCAGGCATGAGC
    ATTGAAAAAGTTATAAATGTATTTGGGAAAAATATAGTCGATGGTGCTGAGGTAGCATTA
    AGCTATGCTTTATTAGGTGGATTTGCAGCATTAATTTCATACAGTGGTATCACAGACTAT
    TTAGTAGGAAAAATTATAAATGCAATTCACGCTGAAAATAGTCGATGGTCAAGAGTTAAA
    GTCAAAGTGACAATAATCATTGCATTATTAGCTATGAGTATCATGAGTCAAAACTTAATT
    CCTGTACATATTGCATTCATTCCAATTGTCATCCCACCATTGTTAAGTCTGTTTAATGAC
    TTAAAAATAGATAGACGTTTAATCGGTTTGATTATCGGTTTTGGTTTATGTTTCCCGTAT
    GTGTTATTACCATATGGATTCGGTCAAATTTTCCAGCAAATTATTCAAAGTGGCTTTGCA
    AAGGCAAATCACCCAATTGAGTTTAATATGATTTGGAAAGCAATGCTTATTCCTTCAATG
    GGGTATATTGTTGGCTTACTTATCGGTTTATATGTATATCGTAAACCACGTGAATATGAA
    ACACGTAAAATTTCAGATAGTGACAATGTTACAGAGTTAAAACCATATATCTTAATAGTA
    ACAATTGTAGCAATACTAGCTACATTTTTAGTACAAACATTTACAGATTCAATGATTTTT
    GGTGCACTGGCAGGGGTACTCGTATTCTTTATTTCACGTGCATATAATTGGTATGAATTA
    GATGCTAAGTTTGTTGAAGGTATTAAAATTATGGCTTATATTGGTGTAGTTATTTTAACA
    GCAAATGGATTTGCTGGTGTAATGAATGCTACTGGTGATATAGATGAATTAGTTAAAACT
    TTAACAAGTATTACTGGTGATAATAAATTATTTAGCATTATCATGATGTATGTGATAGGT
    TTAATTGTCACTTTAGGTATTGGATCATCATTTGCAACAATTCCTATTATCGCATCATTA
    TTCATTCCTTTTGGAGCGTCAATTGGACTAGATACAATGGCATTAATCGCATTGATTGGA
    ACAGCGAGTGCATTAGGTGACTCAGGTTCGCCTGCAAGTGATTCAACATTAGGACCAACT
    GCGGGATTAAATGTTGATGGCCAACATGATCATATACGTGATACATGTGTACCAAACTTC
    TTGTTTTATAATATTCCTTTAATGATTTTCGGTACTATTGCTGCTATGGTACTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1317

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0813 [new locus tag: NWMN_RS04600 ]
  • symbol: NWMN_0813
  • description: Na+/H+ antiporter family protein
  • length: 438
  • theoretical pI: 6.6689
  • theoretical MW: 47279.2
  • GRAVY: 0.940868

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsTRAP transporter, DctM subunit (TIGR00786; HMM-score: 23.7)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidstransporter, gluconate:H+ symporter (GntP) family (TIGR00791; HMM-score: 11.4)
  • TheSEED:  
    Histidine permease YuiF 
  • PFAM:
    IT (CL0182) Na_H_antiporter; Na+/H+ antiporter family (PF03553; HMM-score: 223.9)
    Na_H_antiport_2; Na+-H+ antiporter family (PF13726; HMM-score: 110.5)
    DctM; Tripartite ATP-independent periplasmic transporter, DctM component (PF06808; HMM-score: 39.8)
    DcuC; C4-dicarboxylate anaerobic carrier (PF03606; HMM-score: 22.5)
    no clan definedDUF969; Protein of unknown function (DUF969) (PF06149; HMM-score: 16.1)
    AzlD; Branched-chain amino acid transport protein (AzlD) (PF05437; HMM-score: 13)
    IT (CL0182) GntP_permease; GntP family permease (PF02447; HMM-score: 12.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 11
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.17
    • Ymax_pos: 11
    • Cmax: 0.146
    • Cmax_pos: 50
    • Smax: 0.36
    • Smax_pos: 7
    • Smean: 0.301
    • D: 0.221
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MINAVVIAVILMIVLCLCRLNVVISLFISALVGGLISGMSIEKVINVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVKVTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPYVLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPREYETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYNWYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSIIMMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPASDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: NWMN_1845 (HisR*)
    HisR* (TF) important in Histidine biosynthesisRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]