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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0883 [new locus tag: NWMN_RS04945 ]
  • pan locus tag?: SAUPAN003187000
  • symbol: NWMN_0883
  • pan gene symbol?:
  • synonym:
  • product: Na+/alanine symporter family protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0883 [new locus tag: NWMN_RS04945 ]
  • symbol: NWMN_0883
  • product: Na+/alanine symporter family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 979147..980775
  • length: 1629
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    TTGAAGTGTCTCGTGCTTAATCGTATAATATTTTTATGTTTAAACAATAGGGGGAATCTT
    ATGATTGAAAAATTAGTAACCTTTTTAAATGAGGTTGTTTGGAGTAAGCCATTAGTTTAT
    GGTTTGCTAATTACTGGTGTGCTATTTACATTGCGTATGCGATTTTTTCAAGTTAGACAT
    TTTAAAGAAATGATTCGATTAATGTTTCAAGGAGAGAAGTCTCCTAATGGTATTTCAAGT
    TTTCAAGCGATAGCCATGTCTTTAGCAGGCAGGGTTGGTACAGGTAATATTGTCGGTGTA
    TCTACTGCAATATTTATAGGAGGACCTGGTGCAGTATTTTGGATGTGGATTACTGCGTTT
    TTAGGTGCAAGTAGTGCTTTTATTGAATCTACACTTGGTCAAATATTCAAGAGAGTTGAA
    AATAATGAATACCGTGGTGGACCAGCGTATTATATTGAATATGGTATTGGTGGTAAATTT
    GGTAAAATTTACGGAATTATCTTTGCTATTGTTACGATTATCTCAGTAGGTCTATTGCTT
    CCTGGTGTGCAATCTAACGCTATAGCAAGTTCTATGCATAATGCGATTCATGTTCCACAA
    TGGTTAATGGGTGGTATTGTTGTAGTTATTTTGGGATTAATTATTTTTGGTGGTGTACGT
    AGTATTGCCAATGTTGCAACAGCCGTTGTACCATTTATGGCAATTATTTACATACTGATG
    GCTGTCATTATCATTTGTATCAATATACAAGAAGTGCCAGCGTTATTTGCATTAATTTTC
    AAATCAGCATTTGGATTACAATCTGCTTTTGGTGGTATCGTTGGCGCAATGATAGAGATT
    GGTGTTAAACGTGGATTATATTCAAATGAGGCTGGTCAAGGTACAGGTCCACACGCAGCA
    GCGGCAGCAGAAGTATCACATCCAAGTAAACAAGGTCTAGTACAAGCATTTTCAGTTTAT
    ATTGATACATTATTTGTATGTACTGCAACTGCTCTGATTATACTTATTTCTGGTACATAT
    AATGTGACTGATGGTACGGTTAATGCGAATGGCACACCGCATTTAATTAAAGATGGCGGT
    ATTTATGTTGAAAATGCAACAGGTAAAGATTATTCAGGTACTGCGATGTATGCACAAGCC
    GGCATTGATAAAGCGTTCCATGGCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTA
    GGTTCGTACTTTATTGCATTTGCTTTATTCTTCTTTGCATTTACTACAATTTTGTCGTAC
    TACTACATTACAGAAACAAATGTTGCTTATTTAACGCGTAATCAAAATAATCAAGTTTCA
    TCGATATTTATTAATATTGCTCGTGTGATTATTTTGTTCGCTACATTTTACGGTGCAGTT
    AAAACAGCTGATGTAGCATGGGCATTCGGTGATTTAGGTGTAGGTCTAATGGCTTGGTTA
    AATATCATTGCGATTTGGATTTTACATAAGCCTGCCGTAAATGCTTTAAAAGATTATGAA
    ATTCAAAAGAAACGTTTAGGCAACGGTTATAATGCAGTTTATCAACCTGATCCGAATAAA
    TTACCTAATGCTGTCTTTTGGTTGAAGACGTATCCAGAACGTTTAAAACAAGCACGTGCC
    AAAAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1629

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0883 [new locus tag: NWMN_RS04945 ]
  • symbol: NWMN_0883
  • description: Na+/alanine symporter family protein
  • length: 542
  • theoretical pI: 9.74281
  • theoretical MW: 58961.7
  • GRAVY: 0.53524

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    amino acid carrier protein (TIGR00835; HMM-score: 465.2)
  • TheSEED:  
    sodium/alanine symporter family protein 
  • PFAM:
    APC (CL0062) Na_Ala_symp; Sodium:alanine symporter family (PF01235; HMM-score: 501.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.166
    • Ymax_pos: 11
    • Cmax: 0.117
    • Cmax_pos: 23
    • Smax: 0.315
    • Smax_pos: 5
    • Smean: 0.277
    • D: 0.209
  • predicted transmembrane helices (TMHMM): 10

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKCLVLNRIIFLCLNNRGNLMIEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEMIRLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTAIFIGGPGAVFWMWITAFLGASSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLLPGVQSNAIASSMHNAIHVPQWLMGGIVVVILGLIIFGGVRSIANVATAVVPFMAIIYILMAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTGPHAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPHLIKDGGIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVGSYFIAFALFFFAFTTILSYYYITETNVAYLTRNQNNQVSSIFINIARVIILFATFYGAVKTADVAWAFGDLGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGNGYNAVYQPDPNKLPNAVFWLKTYPERLKQARAKK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CodY
    CodY (TF) important in Amino acid metabolism  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]