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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0918 [new locus tag: NWMN_RS05140 ]
  • pan locus tag?: SAUPAN003251000
  • symbol: sspA
  • pan gene symbol?: sspA
  • synonym:
  • product: V8 protease, glutamyl endopeptidase precursor

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0918 [new locus tag: NWMN_RS05140 ]
  • symbol: sspA
  • product: V8 protease, glutamyl endopeptidase precursor
  • replicon: chromosome
  • strand: -
  • coordinates: 1016189..1017199
  • length: 1011
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAGGTAAATTTTTAAAAGTTAGTTCTTTATTCGTTGCAACTTTGACAACAGCGACA
    CTTGTGAGTTCTCCAGCAGCAAACGCGTTATCTTCAAAGGCTATGGACAATCATCCACAA
    CAAACGCAGTCAAGCAAACAGCAAACACCTAAGATTCAAAAAGGCGGTAACCTTAAACCA
    TTAGAACAACGTGAACACGCAAATGTTATATTACCAAATAACGATCGTCACCAAATCACA
    GATACAACGAATGGTCATTATGCACCCGTAACTTATATTCAAGTTGAAGCACCTACTGGT
    ACATTTATTGCTTCCGGTGTAGTTGTAGGTAAAGATACTCTTTTAACAAATAAACACGTC
    GTAGATGCTACGCACGGTGATCCTCATGCTTTAAAAGCATTCCCTTCTGCAATTAACCAA
    GACAATTATCCAAATGGTGGTTTCACTGCTGAACAAATCACTAAATATTCAGGCGAAGGT
    GATTTAGCAATAGTTAAATTCTCCCCTAATGAGCAAAACAAACATATTGGTGAAGTAGTT
    AAACCAGCAACAATGAGTAATAATGCTGAAACACAAGTTAACCAAAATATTACTGTAACA
    GGATATCCTGGTGATAAACCTGTAGCAACAATGTGGGAAAGTAAAGGAAAAATCACTTAC
    CTCAAAGGCGAAGCTATGCAATATGATTTAAGTACAACTGGTGGTAATTCAGGTTCACCT
    GTATTTAATGAAAAAAATGAAGTGATCGGAATTCATTGGGGCGGTGTACCAAATGAATTT
    AATGGTGCGGTATTTATTAATGAAAATGTACGCAACTTCTTAAAACAAAATATTGAAGAT
    ATCCATTTTGCCAACGATGACCAACCTAATAACCCAGATAATCCTGATAACCCTAACAAT
    CCTGATAACCCTAACAACCCAGATGAACCAAATAACCCTGACAACCCTAACAACCCTGAT
    AATCCAGACAATGGCGATAACAATAATTCAGACAATCCAGATGCAGCTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1011

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0918 [new locus tag: NWMN_RS05140 ]
  • symbol: SspA
  • description: V8 protease, glutamyl endopeptidase precursor
  • length: 336
  • theoretical pI: 4.80889
  • theoretical MW: 36325.4
  • GRAVY: -0.839881

Function[edit source | edit]

  • reaction:
    EC 3.4.21.-?  ExPASy
  • TIGRFAM:
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidespeptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 18.1)
    Genetic information processingProtein fateProtein folding and stabilizationpeptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 18.1)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesperiplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 13.3)
    Signal transductionRegulatory functionsProtein interactionsperiplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 13.3)
  • TheSEED:  
    Protein MetabolismProtein degradationEC 3.4.21.- Serine endopeptidase Glutamyl endopeptidase precursor (EC 3.4.21.19), serine proteinase SspA 
  • PFAM:
    Peptidase_PA (CL0124) Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 75.1)
    Trypsin; Trypsin (PF00089; HMM-score: 50.2)
    Peptidase_S46; Peptidase S46 (PF10459; HMM-score: 26.9)
    Peptidase_S7; Peptidase S7, Flavivirus NS3 serine protease (PF00949; HMM-score: 22.2)
    Peptidase_C3; 3C cysteine protease (picornain 3C) (PF00548; HMM-score: 18.4)
    Peptidase_S32; Equine arteritis virus serine endopeptidase S32 (PF05579; HMM-score: 17.2)
    Peptidase_S39; Peptidase S39 (PF02122; HMM-score: 16.1)
    Peptidase_CA (CL0125) Phytochelatin; Phytochelatin synthase (PF05023; HMM-score: 11.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 10
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: -0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: VSSPAANA
  • SignalP: Signal peptide length 30 aa
    • Ymax: 0.552
    • Ymax_pos: 30
    • Cmax: 0.329
    • Cmax_pos: 30
    • Smax: 0.982
    • Smax_pos: 13
    • Smean: 0.944
    • D: 0.705
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQSSKQQTPKIQKGGNLKPLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTFIASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEGDLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKITYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQNIEDIHFANDDQPNNPDNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDNNNSDNPDAA

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]