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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0979 [new locus tag: NWMN_RS05485 ]
  • pan locus tag?: SAUPAN003341000
  • symbol: pycA
  • pan gene symbol?: pycA
  • synonym:
  • product: pyruvate carboxylase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0979 [new locus tag: NWMN_RS05485 ]
  • symbol: pycA
  • product: pyruvate carboxylase
  • replicon: chromosome
  • strand: +
  • coordinates: 1085501..1088971
  • length: 3471
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    TTGAGGAGTGTATCGCTATTGAAACAAATAAAAAAGTTACTTGTTGCTAACCGTGGAGAA
    ATTGCAATTCGTATATTCAGAGCGGCGGCAGAATTAGACATCAGCACAGTTGCAATTTAT
    TCGAATGAAGACAAAAGTTCATTACATAGATATAAAGCAGATGAATCCTATTTAGTTGGA
    AGTGATTTAGGTCCTGCTGAAAGTTATTTAAATATTGAGCGTATCATTGATGTAGCAAAA
    CAAGCGAATGTGGATGCGATTCATCCTGGCTATGGATTTTTAAGTGAAAATGAACAATTT
    GCGCGTCGTTGTGCAGAAGAAGGAATTAAATTTATTGGTCCTCATTTAGAACATTTAGAT
    ATGTTTGGAGATAAAGTTAAAGCTCGTACAACGGCTATCAAGGCAGATTTACCAGTTATT
    CCTGGTACAGACGGTCCAATTAAATCATATGAATTAGCAAAAGAATTTGCAGAAGAAGCT
    GGTTTCCCGCTAATGATTAAAGCCACAAGTGGTGGCGGCGGTAAAGGTATGAGAATCGTT
    CGTGAAGAAAGTGAATTAGAAGATGCTTTCCATAGAGCAAAATCAGAAGCTGAAAAATCA
    TTTGGTAATAGTGAAGTTTACATAGAAAGATACATTGATAATCCAAAGCATATTGAAGTA
    CAAGTCATAGGTGACGAACATGGAAATATCGTACACTTATTTGAACGTGATTGTTCAGTA
    CAACGTCGTCATCAAAAAGTTGTAGAAGTTGCACCATCAGTTGGATTATCATCAACATTA
    CGTCAACGTATTTGTGATGCTGCAATTCAATTGATGGAAAATATTAAATATGTCAATGCA
    GGTACTGTTGAATTTCTAGTATCTGGTGACGAATTCTTCTTTATAGAAGTTAACCCTCGT
    GTACAAGTAGAGCATACAATTACAGAGATGGTAACAGGAATTGATATTGTTAAGACACAA
    ATTTTAGTTGCAGCAGGTGCCGATTTATTTGGTGAAGAGATTAATATGCCGCAACAAAAA
    GATATTACAACATTAGGCTATGCCATCCAATGTCGTATTACAACAGAAGATCCGTTAAAT
    GATTTCATGCCGGATACTGGAACAATCATTGCTTATCGTTCAAGCGGGGGCTTTGGTGTA
    CGTCTTGATGCTGGAGATGGTTTCCAAGGTGCTGAGATATCACCTTATTATGATTCATTA
    CTCGTAAAATTATCTACACACGCGATATCATTTAAACAAGCAGAAGAAAAAATGGTACGC
    TCATTACGAGAAATGCGTATTCGTGGTGTTAAAACTAATATTCCATTCTTAATTAATGTA
    ATGAAGAATAAAAAGTTCACAAGTGGTGATTACACAACTAAATTTATTGAAGAAACACCA
    GAACTTTTCGACATTCAGCCGTCTCTAGATAGAGGTACTAAAACATTAGAATATATAGGT
    AATGTAACAATTAATGGTTTCCCAAATGTTGAGAAACGTCCGAAACCAGACTATGAATTA
    GCATCAATTCCAACTGTATCTTCAAGTAAAATCGCTTCATTTAGTGGTACGAAACAATTG
    CTTGATGAAGTAGGTCCAAAAGGTGTAGCTGAATGGGTTAAAAAGCAGGATGATGTCTTA
    CTAACAGATACAACCTTTAGAGATGCACACCAATCATTATTAGCTACACGAGTTAGAACT
    AAGGATATGATTAATATCGCATCCAAAACAGCGGACGTATTTAAAGATGGTTTCTCACTA
    GAAATGTGGGGCGGTGCTACATTTGATGTGGCATATAATTTCTTGAAGGAAAACCCATGG
    GAACGACTTGAACGTCTACGTAAAGCTATTCCAAATGTATTATTCCAAATGTTGTTACGT
    GCTTCAAACGCAGTTGGTTATAAAAACTATCCTGATAATGTTATTCATAAATTCGTACAA
    GAAAGTGCTAAAGCAGGCATAGATGTCTTTAGAATTTTCGATTCATTAAACTGGGTAGAT
    CAAATGAAAGTTGCCAATGAAGCAGTACAAGAAGCGGGCAAAATCTCAGAAGGTACTATT
    TGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTAT
    GTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATG
    GCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTGAGTTAAAATCAGCTGTA
    GATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATACAAA
    CAAGCAATAGATGCTGGTGTCGATATCATTGATACTGCTGTTGCTTCAATGAGTGGTTTA
    ACAAGTCAGCCAAGCGCCAATTCGTTATATTATGCATTAAATGGCTTCCCACGCCACCTT
    AGAACTGATATTGAAGGTATGGAGTCACTTAGTCATTATTGGTCAACTGTACGTACTTAT
    TATTCAGACTTTGAAAGTGATATCAAATCACCGAATACTGAAATTTATCAACATGAAATG
    CCTGGTGGACAGTATTCGAATTTAAGTCAACAAGCTAAAAGTTTAGGTTTAGGCGAAAGA
    TTTGATGAAGTCAAAGATATGTATCGCAGAGTGAATTTCTTATTTGGTGATATCGTAAAA
    GTAACACCATCGTCTAAAGTAGTTGGTGATATGGCACTTTATATGGTACAAAATGATCTT
    GATGAACAATCCGTGATTACAGATGGCTATAAATTAGATTTCCCAGAATCAGTAGTGTCG
    TTCTTCAAAGGTGAAATAGGACAACCTGTAAATGGTTTTAATAAAGATTTACAAGCGGTT
    ATTTTAAAAGGCCAAGAAGCACTAACAGCTCGTCCAGGTGAATATCTAGAGCCAGTTGAT
    TTTGAAAAAGTCCGTGAGTTGCTTGAAGAAGAGCAACAAGGTCCTGTTACGGAGCAAGAT
    ATTATTAGTTATGTACTATATCCAAAAGTATATGAACAATATATTCAAACTAGAAATCAA
    TACGGAAACTTATCGTTACTTGATACGCCTACATTCTTCTTTGGAATGCGTAATGGTGAA
    ACAGTAGAAATCGAAATCGATAAAGGTAAACGATTAATTATTAAACTAGAAACGATTAGT
    GAACCAGATGAAAATGGTAATAGAACGATTTACTATGCGATGAATGGTCAAGCGAGACGT
    ATTTACATTAAAGATGAAAATGTGCATACAAATGCGAACGTTAAGCCAAAAGCAGATAAG
    AGTAATCCAAGTCATATCGGTGCTCAAATGCCAGGTTCAGTAACTGAAGTCAAGGTTAGT
    GTAGGTGAAACTGTGAAAGCTAATCAGCCGTTGCTAATTACTGAAGCTATGAAAATGGAA
    ACAACAATTCAAGCACCATTTGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGAC
    ACAATAGCGACAGGCGATTTATTAATCGAAATTGAAAAAGCAACTGACTAA
    60
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    2160
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    2700
    2760
    2820
    2880
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    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3471

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0979 [new locus tag: NWMN_RS05485 ]
  • symbol: PycA
  • description: pyruvate carboxylase
  • length: 1156
  • theoretical pI: 4.95558
  • theoretical MW: 129203
  • GRAVY: -0.319291

Function[edit source | edit]

  • reaction:
    EC 6.4.1.1?  ExPASy
    Pyruvate carboxylaseATP + pyruvate + HCO3(-) = ADP + phosphate + oxaloacetate
  • TIGRFAM:
    MetabolismEnergy metabolismGlycolysis/gluconeogenesispyruvate carboxylase (TIGR01235; EC 6.4.1.1; HMM-score: 1797.3)
    MetabolismFatty acid and phospholipid metabolismBiosynthesisacetyl-CoA carboxylase, biotin carboxylase subunit (TIGR00514; EC 6.3.4.14; HMM-score: 547.7)
    MetabolismCentral intermediary metabolismNitrogen metabolismurea carboxylase (TIGR02712; EC 6.3.4.6; HMM-score: 532.2)
    MetabolismTransport and binding proteinsCations and iron carrying compoundsoxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 487.6)
    MetabolismEnergy metabolismOtheroxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 487.6)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPyrimidine ribonucleotide biosynthesiscarbamoyl-phosphate synthase, large subunit (TIGR01369; EC 6.3.5.5; HMM-score: 68.6)
    MetabolismEnergy metabolismPyruvate dehydrogenasedihydrolipoyllysine-residue acetyltransferase (TIGR01348; EC 2.3.1.12; HMM-score: 43.4)
    alpha-L-glutamate ligase, RimK family (TIGR00768; EC 6.3.2.-; HMM-score: 42.8)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanD-alanine--D-alanine ligase (TIGR01205; EC 6.3.2.4; HMM-score: 41.5)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylglycinamide formyltransferase 2 (TIGR01142; EC 2.1.2.-; HMM-score: 39.3)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylaminoimidazole carboxylase, ATPase subunit (TIGR01161; EC 4.1.1.21; HMM-score: 39.2)
    MetabolismTransport and binding proteinsUnknown substrateefflux transporter, RND family, MFP subunit (TIGR01730; HMM-score: 34.3)
    MetabolismFatty acid and phospholipid metabolismBiosynthesisacetyl-CoA carboxylase, biotin carboxyl carrier protein (TIGR00531; HMM-score: 34)
    homocitrate synthase (TIGR02146; EC 2.3.3.14; HMM-score: 32.3)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOtherhomocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 32)
    MetabolismCentral intermediary metabolismNitrogen fixationhomocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 32)
    lysine biosynthesis enzyme LysX (TIGR02144; HMM-score: 28.9)
    isopropylmalate/citramalate/homocitrate synthases (TIGR02090; HMM-score: 26.5)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylamine--glycine ligase (TIGR00877; EC 6.3.4.13; HMM-score: 25.6)
    MetabolismEnergy metabolismTCA cycledihydrolipoyllysine-residue succinyltransferase, E2 component of oxoglutarate dehydrogenase (succinyl-transferring) complex (TIGR01347; EC 2.3.1.61; HMM-score: 23.7)
    MetabolismEnergy metabolismOther4-hydroxy-2-oxovalerate aldolase (TIGR03217; EC 4.1.3.39; HMM-score: 23.7)
    Cellular processesCellular processesBiosynthesis of natural productscyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 23.2)
    2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase (TIGR02927; EC 2.3.1.61; HMM-score: 22)
    MetabolismTransport and binding proteinsOtherefflux pump membrane protein (TIGR00998; HMM-score: 18.9)
    Cellular processesCellular processesBiosynthesis of natural productsNHLM bacteriocin system secretion protein (TIGR03794; HMM-score: 15.9)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesNHLM bacteriocin system secretion protein (TIGR03794; HMM-score: 15.9)
    MetabolismAmino acid biosynthesisOtherpyrrolysine biosynthesis protein PylC (TIGR03909; HMM-score: 15.8)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtype I secretion membrane fusion protein, HlyD family (TIGR01843; HMM-score: 13.9)
    MetabolismTransport and binding proteinsOthermembrane fusion protein cluster 2 protein (TIGR00999; HMM-score: 12.7)
  • TheSEED:  
    CarbohydratesCentral carbohydrate metabolismPyruvate metabolism I: anaplerotic reactions, PEP Pyruvate carboxylase (EC 6.4.1.1) 
  • PFAM:
    ATP-grasp (CL0179) CPSase_L_D2; Carbamoyl-phosphate synthase L chain, ATP binding domain (PF02786; HMM-score: 259.1)
    Post-HMGL (CL0597) PYC_OADA; Conserved carboxylase domain (PF02436; HMM-score: 247)
    PreATP-grasp (CL0483) Biotin_carb_N; Biotin carboxylase, N-terminal domain (PF00289; HMM-score: 145.6)
    Hybrid (CL0105) Biotin_carb_C; Biotin carboxylase C-terminal domain (PF02785; HMM-score: 111.7)
    TIM_barrel (CL0036) HMGL-like; HMGL-like (PF00682; HMM-score: 94.3)
    Hybrid (CL0105) Biotin_lipoyl; Biotin-requiring enzyme (PF00364; HMM-score: 68.7)
    ATP-grasp (CL0179) ATPgrasp_Ter; ATP-grasp in the biosynthetic pathway with Ter operon (PF15632; HMM-score: 48.7)
    ATP-grasp; ATP-grasp domain (PF02222; HMM-score: 43.4)
    Dala_Dala_lig_C; D-ala D-ala ligase C-terminus (PF07478; HMM-score: 37.7)
    Hybrid (CL0105) Biotin_lipoyl_2; Biotin-lipoyl like (PF13533; HMM-score: 37.3)
    ATP-grasp (CL0179) ATP-grasp_3; ATP-grasp domain (PF02655; HMM-score: 33.2)
    Hybrid (CL0105) HlyD_D23; Barrel-sandwich domain of CusB or HlyD membrane-fusion (PF16576; HMM-score: 28)
    HlyD_3; HlyD family secretion protein (PF13437; HMM-score: 26.1)
    ATP-grasp (CL0179) RimK; RimK-like ATP-grasp domain (PF08443; HMM-score: 24)
    ATP-grasp_4; ATP-grasp domain (PF13535; HMM-score: 16.2)
    GARS_A; Phosphoribosylglycinamide synthetase, ATP-grasp (A) domain (PF01071; HMM-score: 14.1)
    Hybrid (CL0105) RnfC_N; RnfC Barrel sandwich hybrid domain (PF13375; HMM-score: 14)
    GCV_H; Glycine cleavage H-protein (PF01597; HMM-score: 12.8)
    POTRA (CL0191) YqfD; Putative stage IV sporulation protein YqfD (PF06898; HMM-score: 10.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: biotin
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.209
    • Ymax_pos: 11
    • Cmax: 0.115
    • Cmax_pos: 22
    • Smax: 0.609
    • Smax_pos: 1
    • Smean: 0.397
    • D: 0.282
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MRSVSLLKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHRYKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEGIKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMIKATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIGDEHGNIVHLFERDCSVQRRHQKVVEVAPSVGLSSTLRQRICDAAIQLMENIKYVNAGTVEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQKDITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYDSLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFIEETPELFDIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASFSGTKQLLDEVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTADVFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNYPDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILNPERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKSAVDLPIHLHTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDIEGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFDEVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVSFFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTEQDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKLETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSVTEVKVSVGETVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIEKATD

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CodY
    CodY (TF) important in Amino acid metabolism  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]