From AureoWiki
Jump to navigation Jump to search
PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1028 [new locus tag: NWMN_RS05820 ]
  • pan locus tag?: SAUPAN001695000
  • symbol: NWMN_1028
  • pan gene symbol?:
  • synonym:
  • product: phage tape measure protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1028 [new locus tag: NWMN_RS05820 ]
  • symbol: NWMN_1028
  • product: phage tape measure protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1124831..1128295
  • length: 3465
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    GTGACGGAATATAAAATTAAAGCGACTATTGAAGCTAGTGTAGCCAAATTCAAAAGGCAA
    ATCGATAGTGCGGTTAAGTCTGTGCAAAGATTTAAACGAGTAGCAGATCAAACTAAAGAT
    GTCGAATTAAATGCTGATGATAAAAAATTACAAAAAACTATCAAAGTTGCTAAAAAGTCT
    TTAGATGCCTTTAGTAACAAAAAAGTAAAAGCTAAATTAGATGCTAGTATACAAGACTTG
    CAACAAAAGGTACTAGAATCGAATTTTGAACTAGACAAACTAAACTCTAAAGAAGTTACT
    CCAGAGATTAAATTACAAAAACAAAAATTGACTAAAGATATCGCTGAAGCAGAAGCTAAG
    TTATCCGAACTAGAAAAGAAGCGTGTCAATATTGACATCAATGCAGATAACAGTAAATTC
    AATCGAGTGTTAAAAGTATCTAAAGCTAGTCTTGAAGCATTAAATAGGTCTAAAGCCAAA
    GCTATTATAGACGTGGACAATGGTGTTGCTAACTCTAAAATAAAACGCACTAAAGAAGAA
    CTTAAAAGTATTCCAAACAAAACTAGATCTCGACTAGATGTAGATACAGGACTTTCTATA
    CCAACTATTTATGCGTTTAAAAAATCATTAGACGCATTGCCAAACAAAAAAACAACGAAG
    GTAGATGTCGATACTAACGGTTTAAAGAAAGCTTATGCCTACATAATAAAAGTAAACGAC
    AATTTCCAAAGACAAATGGGGAATTTAGCTAATATGTTTCGCGTGTTCGGCACTGTAGGT
    TCTAATATGGTTGGTGGATTACTTACATCATCTTTTAGTATCTTAATACCTGTAATAGCG
    AGCGTAGTACCTGTAGTATTTGCGCTATTAAACGCTATCAAAGTGTTAACTGGCGGTGTA
    CTTGCTTTAGGTGGTGCTGTAGCAATAGCTGGTGCTGGCTTTGTAGCATTTGGCGCAATG
    GCTATCAGCGCTATAAAGATGCTTAGTGACGGCACTTTACAAGCTAGCTCAGCAACAAAC
    GAATACAAAAAAGCTTTAGATGGCGTAAAGTCAGCATGGACTGATATTATAAAGCAAAAT
    CAATCCGCTATCTTCACAACTCTTGCAAATGGTTTAAATACTGTTAAAACAGCAATGCAG
    AGCTTACAACCGTTTTTTAGTGGTATTTCAAGAGGAATGGAAGAGGCGTCTCAAAGTGTA
    CTTAAATGGGCTCAAAATAGCGGTGTAGCATCAAGGTTCTTCAACATGATGAATACAACT
    GGTGTTTCGGTATTTAACAAGCTATTAAGTGCTGCAGGAGGTTTCGGTGACGGATTAGTC
    AATGTGTTCACACAATTAGCACCACTGTTTCAATGGTCGGCTGATTGGTTGGATAGATTA
    GGTCAATCATTCTCTAATTGGGCTAATAGTGCAGCTGGAGAAAATTCGATAACTCGTTTT
    ATTGAATACACAAAAACAAACTTACCTATCATTGGTAATATTTTTAAAAATGTTTTCGTT
    GGAATTAACAATTTGATGAATGCATTCAGTGGATCATCAACTGGCATATTCCAATCTCTT
    GAACAAATGACAGCTAAGTTTAGGGAATGGTCTGAACAAGTAGGACAATCTCAAGGGTTT
    AAAGATTTTGTCAGCTATATACAAACTAATGGACCACTAATAATGCAATTAATTGGGAAC
    ATTGCAAGAGGATTAGTTGCATTCGCAACAGCGATGGCTCCTATAGCTAGTGCAGTATTA
    CGCGTTGCAGTAGCAATAACTGGTTGGATAGCTAACTTGTTTGAGGCGCATCCAGCTACA
    GCACAATTAGTTGGCGTCATTATAACTTTAGTTGGTGCATTTAGATTTTTAATTGCTCCA
    ATATTAGCGGTAATGGATTTTCTGGGGCCATTAGCAGCAAGATTAGTCGCATTAGTAACT
    AAGTTTGGTTGGGCTAAAACAGGAACTTTAGTATTAAGTAAGGCAATGACATCATTAAAA
    GGTCCAATAAAATTAGTTACAGCTATATTCCAATTGTTATTCGGTAAGATTGGATTAATT
    AGAAATGCTATCACAGGACTAGTAACTGTGTTTGGTATTTTAGGTGGTCCAATAACAATA
    GTAATTGGTGTAATCGCTGCATTAATAGCTATATTTGTTTTATTGTGGAATAAAAATGAA
    GGATTCAGAAACTTTATTATAAATGTTTGGAATGCGATAAAAACATTTATGGTTACAGTT
    TGGAATGTGTTGAAAACTGTAGCTTCGGTTGTATGGAATGCTATTTTAAAAGCTATCACT
    ACAGCAGTATCAAATGTATACAATTTTATAATGATTATTTGGAATCAAATAGTTGCTTAT
    TTGCAAGGGCTATGGAATGGAATTATCGCTATTGCAACAACAGTATGGAACCTTTTAGTT
    ACAATCATCACAACTGTTTTCACGACGATAATGACAATAGTTATGACGATATGGACAGCT
    ATTTGGACATTCTTAAGTACAATCTGGAACACGATAATTACAATCGCTACTACGATTTGG
    AATTTGTTAGTCACTGTAATAACTACAGTATTTACCACAATTATGACTATCGCAATGACA
    ATTTGGAACGCTATTTGGACGTTCTTACAAACGTTGTGGAACACTATAGTTACTGTGGCA
    ACTAAGGTTTGGAACGCTATCACTACAACTATATCTACTGCGTTACAAGCGGCATGGAGT
    TTTATTTCTAATATATGGAATACGATTTGGAGTTTCTTATCTGGTATATTAACGACAATT
    TGGAATAAAGTTGTAAGCATATTCACACAAGTTGTATCAACTATATCAGACAAAATGTCT
    CAAGCTTGGAACTTCATCGTGACTAAAGGTATGCAATGGGTATCTACTATAACAAGTACG
    CTAATTAACTTTGTTAATAGAGTTATTCAAGGATTCGTTAATGTTGTAAACAAAGTTAGT
    CAAGGTATGACAAATGCAGTAAATAAAATAAAAAGTTTTATAGGAGATTTTGTGTCTGCA
    GGTGCTGATATGATCCGTGGTTTAATTAGAGGTATTGGACAAATGGCTGGTCAATTAGTA
    GATGCAGCTAAAAATGTTGCTAAGAAAGCTTTAGATGCAGCTAAAAGTGCTTTGGGTATT
    CACTCACCTTCACGTGAATTCATGGATGTTGGTATGTATTCAATGCTAGGTTTCGTTAAA
    GGTATAGATAATCATTCAAGTAAAGTTATCCGTAATGTTTCTAATGTTGCAGATAAAGTA
    GTTGATGCATTTCAACCTACATTAAACGCACCTGACATTTCTAGTATTACAGGAAACTTA
    AGTAATTTAGGTGGAAATATAAATGCGCAAGTACAACACACACATTCTATTGAAACATCA
    CCGAACATGAAAACTGTTAAAGTTGAATTCGATGTTAATAACGATGCGCTTACTAGTATT
    GTTAACGGCAGAAATGCTAAACGCAATTCTGAGTATTACTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3465

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_1028 [new locus tag: NWMN_RS05820 ]
  • symbol: NWMN_1028
  • description: phage tape measure protein
  • length: 1154
  • theoretical pI: 10.6502
  • theoretical MW: 125759
  • GRAVY: 0.314905

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: data available for NCTC8325
  • PFAM:
    HTH (CL0123) DUF2316; Uncharacterized protein conserved in bacteria (DUF2316) (PF10078; HMM-score: 10.2)
    and 2 more
    Traffic (CL0147) Sec20; Sec20 (PF03908; HMM-score: 8)
    no clan defined Phasin_2; Phasin protein (PF09361; HMM-score: 7.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006002
    • TAT(Tat/SPI): 0.000595
    • LIPO(Sec/SPII): 0.001263
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTEYKIKATIEASVAKFKRQIDSAVKSVQRFKRVADQTKDVELNADDKKLQKTIKVAKKSLDAFSNKKVKAKLDASIQDLQQKVLESNFELDKLNSKEVTPEIKLQKQKLTKDIAEAEAKLSELEKKRVNIDINADNSKFNRVLKVSKASLEALNRSKAKAIIDVDNGVANSKIKRTKEELKSIPNKTRSRLDVDTGLSIPTIYAFKKSLDALPNKKTTKVDVDTNGLKKAYAYIIKVNDNFQRQMGNLANMFRVFGTVGSNMVGGLLTSSFSILIPVIASVVPVVFALLNAIKVLTGGVLALGGAVAIAGAGFVAFGAMAISAIKMLSDGTLQASSATNEYKKALDGVKSAWTDIIKQNQSAIFTTLANGLNTVKTAMQSLQPFFSGISRGMEEASQSVLKWAQNSGVASRFFNMMNTTGVSVFNKLLSAAGGFGDGLVNVFTQLAPLFQWSADWLDRLGQSFSNWANSAAGENSITRFIEYTKTNLPIIGNIFKNVFVGINNLMNAFSGSSTGIFQSLEQMTAKFREWSEQVGQSQGFKDFVSYIQTNGPLIMQLIGNIARGLVAFATAMAPIASAVLRVAVAITGWIANLFEAHPATAQLVGVIITLVGAFRFLIAPILAVMDFLGPLAARLVALVTKFGWAKTGTLVLSKAMTSLKGPIKLVTAIFQLLFGKIGLIRNAITGLVTVFGILGGPITIVIGVIAALIAIFVLLWNKNEGFRNFIINVWNAIKTFMVTVWNVLKTVASVVWNAILKAITTAVSNVYNFIMIIWNQIVAYLQGLWNGIIAIATTVWNLLVTIITTVFTTIMTIVMTIWTAIWTFLSTIWNTIITIATTIWNLLVTVITTVFTTIMTIAMTIWNAIWTFLQTLWNTIVTVATKVWNAITTTISTALQAAWSFISNIWNTIWSFLSGILTTIWNKVVSIFTQVVSTISDKMSQAWNFIVTKGMQWVSTITSTLINFVNRVIQGFVNVVNKVSQGMTNAVNKIKSFIGDFVSAGADMIRGLIRGIGQMAGQLVDAAKNVAKKALDAAKSALGIHSPSREFMDVGMYSMLGFVKGIDNHSSKVIRNVSNVADKVVDAFQPTLNAPDISSITGNLSNLGGNINAQVQHTHSIETSPNMKTVKVEFDVNNDALTSIVNGRNAKRNSEYYL

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]