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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1039 [new locus tag: NWMN_RS05880 ]
  • pan locus tag?: SAUPAN001706000
  • symbol: NWMN_1039
  • pan gene symbol?:
  • synonym:
  • product: phage amidase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1039 [new locus tag: NWMN_RS05880 ]
  • symbol: NWMN_1039
  • product: phage amidase
  • replicon: chromosome
  • strand: +
  • coordinates: 1139902..1141347
  • length: 1446
  • essential: unknown

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGCAAGCAAAATTGACTAAAAAAGAGTTTATAGAGTGGTTGAAAACTTCTGAGGGAAAA
    CAATTTAATATCGACCTTTGGTATGCATTTCAATGCTTTGATTATGCTAATGCTGGTTGG
    AAAGCTTTGTTTGGATTACTCCTAAAAGGTGTAGGCGCAAAAGATATTCCGTTCGCTAAC
    AACTTCGACGGATTAGCTACTGTATACCAAAATACACCGGACTTCTTAGCACAACCTGGC
    GACATGGTGGTATTCGGTAGCAACTACGGTGCTGGATATGGTCACGTTGCATGGGTAATT
    GAAGCAACTTTAGATTACATCATTGTATATGAGCAGAATTGGCTAGGCGGTGGCTGGACT
    GACGGAATCGAACAACCCGGCTGGGGTTGGGAAAAAGTTACAAGACGACAACATGCTTAT
    GATTTCCCTATGTGGTTTATCCGTCCGAATTTTAAAAGTGAGACAGCGCCACGATCAGTT
    CAATCTCCTACACAAGCACCTAAAAAAGAAACAGCTAAGCCACAACCTAAAGCAGTAGAA
    CTTAAAATCATCAAAGATGTGGTTAAAGGTTATGACCTACCTAAGCGTGGTAGTAACCCT
    AAAGGTATAGTTATACACAACGACGCAGGGAGCAAAGGGGCGACTGCTGAAGCATATCGT
    AACGGATTAGTAAATGCACCTTTATCAAGATTAGAAGCGGGCATTGCGCATAGTTACGTA
    TCAGGCAACACAGTTTGGCAAGCCTTAGATGAATCACAAGTAGGTTGGCATACCGCTAAT
    CAAATAGGTAATAAATATTATTACGGTATTGAAGTATGTCAATCAATGGGCGCAGATAAC
    GCGACATTCTTAAAAAATGAACAGGCAACTTTCCAAGAATGTGCTAGGTTATTAAAAAAG
    TGGGGATTACCAGCAAACAGAAATACAATCAGATTGCACAATGAATTTACTTCAACATCA
    TGCCCTCATAGAAGTTCGGTTTTACACACTGGTTTTGACCCAGTAACTCGCGGTCTATTG
    CCAGAAGACAAGCGGTTGCAACTTAAAGACTACTTTATCAAGCAGATTAGGGCGTACATG
    GATGGTAAAATACCGGTTGCCACTGTCTCTAATGAGTCAAGCGCTTCAAGTAATACAGTT
    AAACCAGTTGCGAGTGCATGGAAACGTAATAAATATGGTACTTACTACATGGAAGAAAGT
    GCTAGATTCACAAACGGCAATCAACCAATCACAGTAAGAAAAGTGGGGCCATTCTTATCT
    TGTCCAGTGGGTTATCAGTTCCAACCTGGTGGATATTGTGATTATACAGAAGTGATGTTA
    CAAGATGGTCATGTTTGGGTAGGATATACATGGGAGGGGCAACGTTATTACTTGCCTATT
    AGAACATGGAATGGTTCTTCCCCACCTAATCAGATATTAGGTGACTTATGGGGAGAAATC
    AGTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1446

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1039 [new locus tag: NWMN_RS05880 ]
  • symbol: NWMN_1039
  • description: phage amidase
  • length: 481
  • theoretical pI: 8.78887
  • theoretical MW: 54045.7
  • GRAVY: -0.471518

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    Phage lysin, N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) 
  • PFAM:
    SH3 (CL0010) SH3_5; Bacterial SH3 domain (PF08460; HMM-score: 85.8)
    no clan definedAmidase_2; N-acetylmuramoyl-L-alanine amidase (PF01510; HMM-score: 57.8)
    Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 43.4)
    HTH (CL0123) Myb_DNA-bind_2; Rap1 Myb domain (PF08914; HMM-score: 12.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0.1
    • Cytoplasmic Membrane Score: 0.14
    • Cellwall Score: 0.15
    • Extracellular Score: 9.6
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.149
    • Ymax_pos: 54
    • Cmax: 0.145
    • Cmax_pos: 54
    • Smax: 0.26
    • Smax_pos: 52
    • Smean: 0.096
    • D: 0.128
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MQAKLTKKEFIEWLKTSEGKQFNIDLWYAFQCFDYANAGWKALFGLLLKGVGAKDIPFANNFDGLATVYQNTPDFLAQPGDMVVFGSNYGAGYGHVAWVIEATLDYIIVYEQNWLGGGWTDGIEQPGWGWEKVTRRQHAYDFPMWFIRPNFKSETAPRSVQSPTQAPKKETAKPQPKAVELKIIKDVVKGYDLPKRGSNPKGIVIHNDAGSKGATAEAYRNGLVNAPLSRLEAGIAHSYVSGNTVWQALDESQVGWHTANQIGNKYYYGIEVCQSMGADNATFLKNEQATFQECARLLKKWGLPANRNTIRLHNEFTSTSCPHRSSVLHTGFDPVTRGLLPEDKRLQLKDYFIKQIRAYMDGKIPVATVSNESSASSNTVKPVASAWKRNKYGTYYMEESARFTNGNQPITVRKVGPFLSCPVGYQFQPGGYCDYTEVMLQDGHVWVGYTWEGQRYYLPIRTWNGSSPPNQILGDLWGEIS

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]