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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1045 [new locus tag: NWMN_RS05910 ]
  • pan locus tag?: SAUPAN003364000
  • symbol: isdF
  • pan gene symbol?: isdF
  • synonym:
  • product: iron/heme permease

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1045 [new locus tag: NWMN_RS05910 ]
  • symbol: isdF
  • product: iron/heme permease
  • replicon: chromosome
  • strand: +
  • coordinates: 1148125..1149093
  • length: 969
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGATGATAAAAAATAAAAAGAAACTACTATTTTTATGTTTGTTAGTCATTTTAATCGCA
    ACTGCTTATATTTCGTTTGTAACCGGTACAATTAAATTGTCATTTAATGACCTATTTACA
    AAATTTACAACTGGTAGCAATGAAGCAGTGGATTCAATCATTGATTTGCGATTGCCACGT
    ATATTAATTGCATTGATGGTTGGCGCAATGTTAGCAGTTTCTGGAGCATTATTACAAGCA
    GCACTACAAAATCCTTTGGCAGAGGCGAATATCATTGGCGTTTCCTCAGGTGCACTTATA
    ATGAGAGCACTTTGTATGTTGTTTATTCCACAATTGTACTTTTACTTACCATTATTAAGT
    TTTATTGGAGGTTTAATACCATTTTTAATAATTATATTGTTGCATTCTAAATTTAGATTC
    AATGCTGTAAGTATGATATTAGTAGGTGTTGCGTTATTCGTATTATTAAATGGTGTTTTA
    GAAATTTTAACTCAAAACCCTTTAATGAAAATTCCTCAAGGCTTAACAATGAAAATATGG
    AGCGACGTATACATATTAGCAGTATCAGCATTATTGGGATTAATATTAACATTACTATTG
    TCCCCTAAATTGAATTTACTAAATTTAGACGACATACAAGCGCGAAGTATCGGTTTTAAT
    ATTGATCGTTACAGATGGTTAACAGGTTTATTAGCAGTATTTTTAGCAAGTGCAACTGTT
    GCGATTGTTGGACAACTAGCCTTTTTAGGTATTATTGTGCCACATGTGGTTAGAAAGCTA
    GTTGGGGGCAATTACAGAGTACTTATTCCGTTTTCTACAGTTATTGGTGCATGGCTATTG
    TTAGTGGCTGATTTATTAGGACGAGTGATACAGCCTCCTTTAGAAATTCCAGCCAATGCT
    ATTTTAATGATTGTCGGTGGTCCAATGCTAATTTACTTAATTTGTCAAAGTCAACGAAAT
    CGAATCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    969

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1045 [new locus tag: NWMN_RS05910 ]
  • symbol: IsdF
  • description: iron/heme permease
  • length: 322
  • theoretical pI: 10.3065
  • theoretical MW: 35174.8
  • GRAVY: 1.10248

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    proposed F420-0 ABC transporter, permease protein (TIGR03869; HMM-score: 124.9)
  • TheSEED:  
    Iron acquisition and metabolismIron acquisition and metabolism - no subcategoryHeme, hemin uptake and utilization systems in GramPositives Heme transporter IsdDEF, permease component IsdF 
  • PFAM:
    Membrane_trans (CL0142) FecCD; FecCD transport family (PF01032; HMM-score: 248.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 9
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: 0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide length 12 aa
    • Ymax: 0.272
    • Ymax_pos: 12
    • Cmax: 0.296
    • Cmax_pos: 34
    • Smax: 0.809
    • Smax_pos: 5
    • Smean: 0.743
    • D: 0.456
  • predicted transmembrane helices (TMHMM): 8

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MMIKNKKKLLFLCLLVILIATAYISFVTGTIKLSFNDLFTKFTTGSNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRALCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLEILTQNPLMKIPQGLTMKIWSDVYILAVSALLGLILTLLLSPKLNLLNLDDIQARSIGFNIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGAWLLLVADLLGRVIQPPLEIPANAILMIVGGPMLIYLICQSQRNRI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: NWMN_1406 (Fur*)
    Fur* (TF) important in Iron homeostasis  RegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]