NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1136 [new locus tag: NWMN_RS06410 ]
• pan locus tag^?: SAUPAN003509000
• symbol: NWMN_1136
• pan gene symbol^?: fakA
• synonym:
• product: hypothetical protein

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1136 [new locus tag: NWMN_RS06410 ]
• symbol: NWMN_1136
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 1243951..1245597
• length: 1647
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330694 NCBI
• RefSeq: YP_001332170 NCBI
• BioCyc:
• MicrobesOnline: 3706688 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGATTAGCAAAATTAATGGTAAATTATTTGCCGATATGATTATACAAGGGGCACAAAAT
  TTATCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGAT
  ACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTG
  TCGAAAAATATCGGCGAATTAGGTAAAACATTCTCGAAAGGTTTACTAATGGGTGCAAGA
  GGTAACTCTGGTGTCATCTTGTCACAATTATTCAGAGGATTTTGTAAAAATATTGAAAGT
  GAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCA
  TATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCG
  CAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATT
  ATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAA
  GTTGGTGTTGTTGATAGTGGCGGTAAAGGTTTGTTATGCGTTTACGAAGGATTCTTAAAA
  GCGCTTAAAGGTGAAAAAGTTGAAGCCAAAGTTGCAAAGATAGATAAAGATGAATTTGTA
  CATGATGAACATGATTTCCATGGTGTAATTAATACTGAAGATATTATTTATGGCTATTGT
  ACTGAAATGATGGTTCGTTTTGGAAAGAATAAAAAAGCCTTTGATGAACAAGAATTCAGG
  CAAGATATGAGTCAATTTGGTGATTCTTTATTAGTCATTAATGATGAAGAAATTGTGAAA
  GTTCACGTGCATACCGAATACCCAGGTAAAGTGTTTAATTATGGTCAACAATATGGTGAA
  TTAATTAAACTTAAGGTTGAAAATATGAGAGAACAGCACCGTGAAGTGATTCGAAAAGAA
  CAGCACACAGCTAAACCGAAAATGGAAACGGTTGAAACAGCAATTATTACTATTTCTATG
  GGTGAAGGTATTTCAGAGATATTTAAATCAATGGGTGCCACACATATCATTAGTGGTGGA
  CAAACGATGAATCCTTCTACAGAAGATATCGTTAAAGTCATTGAACAATCAAAATGTAAA
  CGTGCAATTATTTTACCGAATAATAAAAATATCTTAATGGCAAGTGAACAAGCAGCGAGT
  ATTGTTGATGCAGAAGCTGTTGTTATTCCAACGAAATCTATTCCTCAAGGTATAAGCGCA
  CTATTCCAATATGATGTGGACGCAACACTTGAAGAAAATAAAGCGCAAATGGCTGATTCA
  GTAAATAACGTTAAATCTGGTTCATTAACGTACGCTGTTCGTGATACGAAAATTGATGGC
  GTTGAGATTAAAAAAGACGCGTTTATGGGCTTGATTGAAGATAAGATTGTAAGCAGCCAA
  AGTGATCAATTAACAACGGTTACTGAGTTGTTAAATGAGATGTTAGCAGAAGATAGTGAA
  ATATTGACTGTGATTATTGGTCAAGATGCAGAGCAAGCAGTTACAGATAACATGATAAAC
  TGGATCGAAGAGCAATATCCAGATGTAGAAGTGGAAGTTCATGAAGGTGGACAACCAATT
  TATCAATATTTCTTTTCAGTAGAATAA
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  1647
  

⊟Protein

⊟General

• locus tag: NWMN_1136 [new locus tag: NWMN_RS06410 ]
• symbol: NWMN_1136
• description: hypothetical protein
• length: 548
• theoretical pI: 4.3893
• theoretical MW: 60515.3
• GRAVY: -0.240146

⊟Function

• TIGRFAM:
  DAK2 domain fusion protein YloV (TIGR03599; HMM-score: 699)
  and 2 more
  dihydroxyacetone kinase, L subunit (TIGR02365; EC 2.7.-.-; HMM-score: 38.1)
  dihydroxyacetone kinase (TIGR02361; EC 2.7.1.29; HMM-score: 21)
• TheSEED:  
  Clustering-based subsystemsRibosomal Protein L28P relates to a set of uncharacterized proteinsA Gram-positive cluster that relates ribosomal protein L28P to a set of uncharacterized proteins Dihydroxyacetone kinase family protein
  and 1 more
  Fatty Acids, Lipids, and IsoprenoidsPhospholipidsGlycerolipid and Glycerophospholipid Metabolism in Bacteria Dihydroxyacetone kinase family protein
• PFAM:
  EDD (CL0245) Dak1_2; Dihydroxyacetone kinase family (PF13684; HMM-score: 399.4)
  and 3 more
  no clan definedDak2; DAK2 domain (PF02734; HMM-score: 138.7)
  DUF2574; Protein of unknown function (DUF2574) (PF10836; HMM-score: 12.2)
  Hybrid (CL0105) GCV_H; Glycine cleavage H-protein (PF01597; HMM-score: 11.2)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular Possibility: 1
  • Signal Peptide Possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • Ymax: 0.196
  • Ymax_pos: 28
  • Cmax: 0.134
  • Cmax_pos: 28
  • Smax: 0.527
  • Smax_pos: 17
  • Smean: 0.257
  • D: 0.22
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221348 NCBI
• RefSeq: YP_001332170 NCBI
• UniProt: A0A0H3K889 UniProt

⊟Protein sequence

• MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTNMNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESESEINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAIEKANNTEDCIELMEYIIVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKIDKDEFVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDEEIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETAIITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNILMASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLTYAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLAEDSEILTVIIGQDAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1135 > NWMN_1136

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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