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NCBI: 06-JUL-2013

⊟Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1140 [new locus tag: NWMN_RS06430 ]
  • pan locus tag?: SAUPAN003514000
  • symbol: fabD
  • pan gene symbol?: fabD
  • synonym:
  • product: malonyl CoA-acyl carrier protein transacylase

⊟Genome View[edit source | edit]

⊟Gene[edit source | edit]

⊟General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1140 [new locus tag: NWMN_RS06430 ]
  • symbol: fabD
  • product: malonyl CoA-acyl carrier protein transacylase
  • replicon: chromosome
  • strand: +
  • coordinates: 1249606..1250532
  • length: 927
  • essential: unknown other strains

⊟Accession numbers[edit source | edit]

⊟Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGTAAAACAGCAATTATTTTTCCGGGACAAGGTGCCCAAAAAGTTGGTATGGCGCAA
    GATTTGTTTAACAACAATGATCAAGCAACTGAAATTTTAACTTCAGCAGCGAACACATTA
    GACTTTGATATTTTAGAGACAATGTTTACTGATGAAGAAGGTAAATTGGGTGAAACTGAA
    AACACACAACCAGCTTTATTGACGCATAGTTCGGCATTATTAGCAGCGCTAAAAAATTTG
    AATCCTGATTTTACTATGGGGCATAGTTTAGGTGAATATTCAAGTTTAGTTGCAGCTGAC
    GTATTATCATTTGAAGATGCAGTTAAAATTGTTAGAAAACGTGGTCAATTAATGGCGCAA
    GCATTTCCTACTGGTGTAGGAAGCATGGCTGCAGTATTGGGATTAGATTTTGATAAAGTC
    GATGAAATTTGTAAGTCATTATCATCTGATGACAAAATAATTGAACCAGCAAACATTAAT
    TGCCCAGGTCAAATTGTTGTTTCAGGTCACAAAGCTTTAATTGATGAGCTAGTAGAAAAA
    GGTAAATCATTAGGTGCAAAACGTGTCATGCCTTTAGCAGTATCTGGACCATTCCATTCA
    TCGCTAATGAAAGTGATTGAAGAAGATTTTTCAAGTTACATTAATCAATTTGAATGGCGT
    GATGCTAAGTTTCCTGTAGTTCAAAATGTAAATGCGCAAGGTGAAACTGACAAAGAAGTA
    ATTAAATCTAATATGGTCAAGCAATTATATTCACCAGTACAATTCATTAACTCAACAGAA
    TGGCTAATAGACCAAGGTGTTGATCATTTTATTGAAATTGGTCCTGGAAAAGTTTTATCT
    GGCTTAATTAAAAAAATAAATAGAGATGTTAAGTTAACATCAATTCAAACTTTAGAAGAT
    GTGAAAGGATGGAATGAAAATGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    927

⊟Protein[edit source | edit]

⊟General[edit source | edit]

  • locus tag: NWMN_1140 [new locus tag: NWMN_RS06430 ]
  • symbol: FabD
  • description: malonyl CoA-acyl carrier protein transacylase
  • length: 308
  • theoretical pI: 4.52538
  • theoretical MW: 33635
  • GRAVY: -0.146753

⊟Function[edit source | edit]

  • reaction:
    EC 2.3.1.39?  ExPASy
    [Acyl-carrier-protein] S-malonyltransferaseMalonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein]
  • TIGRFAM:
    MetabolismFatty acid and phospholipid metabolismBiosynthesismalonyl CoA-acyl carrier protein transacylase (TIGR00128; EC 2.3.1.39; HMM-score: 328.4)
    and 3 more
    malonate decarboxylase, epsilon subunit (TIGR03131; HMM-score: 201.2)
    polyketide-type polyunsaturated fatty acid synthase PfaA (TIGR02813; HMM-score: 116.5)
    PfaB family protein (TIGR02816; HMM-score: 16.5)
  • TheSEED:  
    Clustering-based subsystemsFatty acid metabolic clusterCOG1399 Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39)
    and 1 more
    Fatty Acids, Lipids, and IsoprenoidsFatty acidsFatty Acid Biosynthesis FASII Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39)
  • PFAM:
    Patatin (CL0323) Acyl_transf_1; Acyl transferase domain (PF00698; HMM-score: 123.7)
    and 1 more
    no clan definedV-ATPase_C; V-ATPase subunit C (PF03223; HMM-score: 11.8)

⊟Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

⊟Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.176
    • Ymax_pos: 20
    • Cmax: 0.144
    • Cmax_pos: 30
    • Smax: 0.303
    • Smax_pos: 18
    • Smean: 0.197
    • D: 0.184
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit source | edit]

⊟Protein sequence[edit source | edit]

  • MSKTAIIFPGQGAQKVGMAQDLFNNNDQATEILTSAANTLDFDILETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVLSFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANINCPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFEWRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPGKVLSGLIKKINRDVKLTSIQTLEDVKGWNEND

⊟Experimental data[edit source | edit]

  • experimentally validated: no data available

⊟Expression & Regulation[edit source | edit]

⊟Operon[edit source | edit]

⊟Regulation[edit source | edit]

  • regulator: FapR* (repression) regulon
    FapR(TF)important in Fatty acid biosynthesis; RegPrecise    transcription unit transferred from N315 data RegPrecise  

⊟Transcription pattern[edit source | edit]

⊟Protein synthesis (provided by Aureolib)[edit source | edit]

⊟Protein stability[edit source | edit]

  • half-life: no data available

⊟Biological Material[edit source | edit]

⊟Mutants[edit source | edit]

⊟Expression vector[edit source | edit]

⊟lacZ fusion[edit source | edit]

⊟GFP fusion[edit source | edit]

⊟two-hybrid system[edit source | edit]

⊟FLAG-tag construct[edit source | edit]

⊟Antibody[edit source | edit]

⊟Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

⊟Literature[edit source | edit]

⊟References[edit source | edit]

⊟Relevant publications[edit source | edit]

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