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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1217 [new locus tag: NWMN_RS06840 ]
  • pan locus tag?: SAUPAN003626000
  • symbol: glnA
  • pan gene symbol?: glnA
  • synonym:
  • product: glutamine synthetase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1217 [new locus tag: NWMN_RS06840 ]
  • symbol: glnA
  • product: glutamine synthetase
  • replicon: chromosome
  • strand: +
  • coordinates: 1342749..1344104
  • length: 1356
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    TTGGAGGATTTTAAAATGCCAAAACGTACTTTCACTAAAGACGACATTCGTAAATTTGCA
    GAAGAGGAAAATGTAAGATATTTAAGATTACAATTCACTGATATTTTAGGAACAATTAAA
    AATGTTGAAGTGCCTGTAAGCCAATTAGAAAAAGTACTTGATAACGAAATGATGTTTGAC
    GGTTCTTCTATCGAAGGTTTCGTACGTATCGAAGAATCAGATATGTACTTACATCCAGAT
    TTAGATACTTGGGTAATCTTCCCATGGACTGCTGGACAAGGTAAAGTTGCACGTTTAATT
    TGTGATGTATATAAAACAGATGGAACACCATTTGAAGGGGATCCTCGTGCAAACTTAAAA
    CGTGTATTAAAAGAAATGGAAGATTTAGGCTTCACAGACTTTAACCTAGGGCCTGAACCA
    GAATTCTTCTTGTTTAAGTTGGATGAAAAAGGGGAACCAACTTTAGAACTTAATGATGAT
    GGTGGATATTTCGATTTAGCACCTACAGATTTAGGTGAAAACTGTCGTCGTGATATTGTT
    TTAGAATTAGAGGATATGGGCTTCGATATTGAAGCTAGTCACCATGAAGTTGCCCCTGGT
    CAACATGAAATTGACTTTAAATATGCAGATGCTGTTACAGCATGTGATAATATCCAAACA
    TTTAAATTGGTTGTTAAAACAATCGCACGTAAACATAATTTACACGCAACATTTATGCCT
    AAACCATTATTCGGTGTGAATGGTAGCGGTATGCACTTTAACGTTTCATTATTCAAAGGT
    AAAGAAAATGCATTCTTTGATCCAAATACTGAAATGGGCTTAACGGAAACTGCATATCAA
    TTTACAGCAGGTGTGCTTAAAAATGCACGCGGATTTACTGCTGTATGTAACCCGTTAGTA
    AACTCATATAAACGTTTAGTACCTGGTTATGAAGCACCATGTTATATTGCATGGAGTGGT
    AAAAACCGTTCACCATTAATCCGTGTACCATCTTCAAGAGGATTATCTACTCGTATCGAA
    GTACGTTCAGTAGATCCAGCTGCAAACCCATACATGGCGTTAGCTGCAATCTTAGAAGCT
    GGACTAGATGGTATTAAAAATAAATTAAAAGTTCCAGAACCAGTTAACCAAAATATTTAC
    GAAATGAACCGTGAAGAACGTGAAGCAGTAGGCATTCAAGACTTACCTTCAACACTTTAT
    ACTGCATTAAAAGCAATGCGTGAAAATGAAGTTATTAAAAAAGCTTTAGGAAATCATATC
    TATAATCAATTTATTAATTCAAAATCAATTGAATGGGATTACTACAGAACTCAAGTCTCT
    GAATGGGAAAGAGATCAGTACATGAAGCAATATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1356

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1217 [new locus tag: NWMN_RS06840 ]
  • symbol: GlnA
  • description: glutamine synthetase
  • length: 451
  • theoretical pI: 4.80101
  • theoretical MW: 51491.1
  • GRAVY: -0.447007

Function[edit source | edit]

  • reaction:
    EC 6.3.1.2?  ExPASy
    Glutamate--ammonia ligaseATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine
  • TIGRFAM:
    MetabolismAmino acid biosynthesisGlutamate familyglutamine synthetase, type I (TIGR00653; EC 6.3.1.2; HMM-score: 581.7)
    glutamine synthetase, type III (TIGR03105; EC 6.3.1.2; HMM-score: 367.9)
  • TheSEED:  
    Amino Acids and DerivativesGlutamine, glutamate, aspartate, asparagine; ammonia assimilationGlutamine synthetases Glutamine synthetase type I (EC 6.3.1.2) 
    Glutamine, glutamate, aspartate, asparagine; ammonia assimilationGlutamine, Glutamate, Aspartate and Asparagine Biosynthesis Glutamine synthetase type I (EC 6.3.1.2) 
    Cell Wall and CapsuleCell Wall and Capsule - no subcategoryPeptidoglycan Biosynthesis Glutamine synthetase type I (EC 6.3.1.2) 
    Nitrogen MetabolismNitrogen Metabolism - no subcategoryAmmonia assimilation Glutamine synthetase type I (EC 6.3.1.2) 
  • PFAM:
    GCS (CL0286) Gln-synt_C; Glutamine synthetase, catalytic domain (PF00120; HMM-score: 453.9)
    no clan definedGln-synt_N; Glutamine synthetase, beta-Grasp domain (PF03951; HMM-score: 110.4)
    Gln-synt_N_2; Glutamine synthetase N-terminal domain (PF16952; HMM-score: 12.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.102
    • Ymax_pos: 49
    • Cmax: 0.113
    • Cmax_pos: 49
    • Smax: 0.145
    • Smax_pos: 2
    • Smean: 0.079
    • D: 0.093
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MEDFKMPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQLEKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTDGTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFDLAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKLVVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQFTAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRIEVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPSTLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulators: CodY, GlnR
    CodY (TF) important in Amino acid metabolism  RegPrecise    transcription unit transferred from N315 data RegPrecise
    GlnR (TF) important in Nitrogen assimilation  RegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]