From AureoWiki
Jump to: navigation, search
NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1230 [new locus tag: NWMN_RS06950 ]
  • pan locus tag?: SAUPAN003683000
  • symbol: NWMN_1230
  • pan gene symbol?: cls1
  • synonym:
  • product: phospholipase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1230 [new locus tag: NWMN_RS06950 ]
  • symbol: NWMN_1230
  • product: phospholipase
  • replicon: chromosome
  • strand: +
  • coordinates: 1353887..1355395
  • length: 1509
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    GTGCCTTTCTTTATAGGAGAAAAGACTATGCGATTTACATTTTCAAACGATTTAGGAACG
    TTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATT
    ATCTTTTTAGAAAGAAATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTT
    TTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCGGCA
    CGCAAATTGAATAAAAACAATGGTAACGTGTTAACGGATTTCGATGGACTTTTAAAACAA
    CAAATAGAAAGCTTTGATAAAGGTAATTATGGTACTGATAACAAACAAGTTCAAAAACAT
    CATGATTTAGTACGTATGCTTTTGATGGATCAAGATGGTTTTTTAACTGAAAATAATAAA
    GTTGATCATTTCATTGATGGAAATGATTTATATGATCAAGTTTTAAAAGATATTAAAAAT
    GCAAAAGAATATATCCATTTAGAGTACTATACTTTCGCTTTAGATGGTTTAGGTAAAAGA
    ATTTTACATGCTTTAGAAGAAAAATTGAAACAAGGTCTAGAAGTAAAAATATTATATGAT
    GATGTTGGATCTAAAAATGTTAAGATGGCAAATTTTGATCATTTTAAATCGTTAGGTGGA
    GAAGTTGAAGCATTTTTTGCTTCAAAATTACCGTTATTGAATTTCAGAATGAATAATAGA
    AATCATAGAAAAATCATCGTAATCGATGGTCAACTAGGTTATGTCGGAGGATTTAACATT
    GGTGATGAATATCTAGGATTAGGAAAATTAGGATATTGGAGAGATACGCATTTACGTATA
    CAAGGGGATGCGGTTGATGCACTGCAGTTGCGATTTATTTTAGACTGGAATTCGCAAGCG
    CACCGTCCACAATTTGAATATGATGTTAAGTATTTCCCTAAAAAGAACGGACCATTGGGC
    AATTCACCAATTCAAATAGCTGCAAGTGGCCCGGCTAGTGACTGGCATCAAATTGAATAC
    GGTTATACAAAAATGATTATGAGTGCAAAGAAATCTGTATATTTACAATCACCATATTTC
    ATTCCGGATAATTCATATATAAATGCCATTAAAATTGCTGCTAAATCAGGTGTAGATGTA
    CATTTAATGATTCCATGTAAGCCAGATCATCCATTAGTATATTGGGCGACATTTTCAAAT
    GCCTCTGACTTATTATCAAGTGGTGTTAAAATTTATACGTATGAAAATGGATTTATACAT
    TCTAAAATGTGCTTAATTGATGATGAAATCGTATCAGTGGGCACAGCAAATATGGACTTT
    AGAAGTTTTGAATTAAATTTTGAAGTAAATGCCTTTGTATATGATGAAAATCTTGCTAAA
    GATTTAAGGGTGGCTTATGAACATGATATTACAAAATCAAAACAACTAACCAAAGAATCA
    TATGCCAATAGACCGCTGTCTGTTAAATTCAAAGAATCGTTAGCAAAATTAGTTTCGCCA
    ATTTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1509

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1230 [new locus tag: NWMN_RS06950 ]
  • symbol: NWMN_1230
  • description: phospholipase
  • length: 502
  • theoretical pI: 7.81952
  • theoretical MW: 57416.6
  • GRAVY: -0.136653

Function[edit source | edit]

  • reaction:
    EC 2.7.8.-?  ExPASy
    2 Phosphatidylglycerol = diphosphatidylglycerol + glycerol?
  • TIGRFAM:
    cardiolipin synthase (TIGR04265; EC 2.7.8.-; HMM-score: 822.2)
  • TheSEED:  
    Fatty Acids, Lipids, and IsoprenoidsPhospholipidsGlycerolipid and Glycerophospholipid Metabolism in Bacteria Cardiolipin synthetase (EC 2.7.8.-) 
  • PFAM:
    PLD (CL0479) PLDc_2; PLD-like domain (PF13091; HMM-score: 145.6)
    PLDc; Phospholipase D Active site motif (PF00614; HMM-score: 51)
    no clan definedPLDc_N; Phospholipase_D-nuclease N-terminal (PF13396; HMM-score: 45)
    CopD_like (CL0430) UPF0093; Uncharacterised protein family (UPF0093) (PF03653; HMM-score: 15.5)
    no clan definedPox_A14; Poxvirus virion envelope protein A14 (PF05767; HMM-score: 14.7)
    DUF3611; Protein of unknown function (DUF3611) (PF12263; HMM-score: 13.6)
    MFS (CL0015) MFS_Mycoplasma; Mycoplasma MFS transporter (PF07672; HMM-score: 13.2)
    no clan definedDUF2207; Predicted membrane protein (DUF2207) (PF09972; HMM-score: 12.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.1
    • Ymax_pos: 12
    • Cmax: 0.146
    • Cmax_pos: 51
    • Smax: 0.139
    • Smax_pos: 2
    • Smean: 0.097
    • D: 0.099
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MPFFIGEKTMRFTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASSTWAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGNVLTDFDGLLKQQIESFDKGNYGTDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEYYTFALDGLGKRILHALEEKLKQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFASKLPLLNFRMNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAVDALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYTKMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNASDLLSSGVKIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVYDENLAKDLRVAYEHDITKSKQLTKESYANRPLSVKFKESLAKLVSPIL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]