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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1440 [new locus tag: NWMN_RS08125 ]
  • pan locus tag?: SAUPAN004113000
  • symbol: gcvPA
  • pan gene symbol?: gcvPA
  • synonym:
  • product: glycine dehydrogenase subunit 1

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1440 [new locus tag: NWMN_RS08125 ]
  • symbol: gcvPA
  • product: glycine dehydrogenase subunit 1
  • replicon: chromosome
  • strand: -
  • coordinates: 1610250..1611605
  • length: 1356
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    GTGTGCATTGTGAGTCATCGTTATATACCTTTAACTGAAAAAGACAAGCAAGAAATGTTA
    CAAACAATTGGTGCAAAATCTATAGGAGAATTATTCGGTGATGTACCAAGTGACATTTTA
    TTAAATAGAGATTTAAATATTGCTGAAGGCGAAGCGGAAACAACGTTACTTAGAAGATTA
    AATCGCATTGCAAGCAAGAATATCACTAAAGAAACGCATACATCATTTTTGGGTGCTGGT
    GTTTATGATCATTACGCGCCATCAGTTGTGGATGCTATGATTTCAAGATCAGAATTTTAC
    ACAGCATATACGCCATATCAACCGGAAATTTCTCAAGGTGAATTACAAGCCATTTTTGAG
    TTCCAAACTTTAATTTGTGAATTAACAGATATGGATGTAGCAAATTCTTCTATGTATGAT
    GGGATGACTAGCTTTGCTGAAGCATGTATCTTAGCATTTAGTCAAACAAAGAAAAATAAA
    ATTGTAGTCTCAAAAGGTTTACATTATCAAGCATTACAAGTACTTCATACTTATGCTAAG
    ACGCGTAAGGAATTCGAAGTAGTGGAAATCGATTTAGATGGCACTGTTACAGATTTGAAA
    AAATTAGAAGCAGCAGTGGATGATGAAACAGCAGCTGTTGCAGTACAATATCCAAACTTT
    TACGGTTCAATTGAAGATCTTGAAAAGATTCATAGCTTTATTGAAGATAAAAAAGCATTA
    TTTATCGTATATGCAAATCCATTAGCATTGGGATTACTTACACCTCCAGGTTCATTTGGT
    GCAGATATTGTTGTAGGTGATACTCAGCCATTCGGAATTCCTGCTCAATTCGGTGGGCCA
    CATTGTGGATATTTTGCAACGACGAAAAAGTTAATGCGTAAAGTGCCAGGTAGATTAGTC
    GGTCAAACACAAGATGATGAAGGTAATCGTGGCTTTGTATTAACATTACAAGCACGTGAA
    CAACATATTCGACGCGATAAAGCGACATCTAATATTTGTTCTAACCAAGCATTAAATGCT
    TTAGCGTCATCAATTGCAATGTCAGCACTTGGTAAACAAGGTATCTATGATATTGCAGTT
    CAAAATATAGAACATGCTAACTATGCTAAACAACAATTTATTAAAAAAGGTTTTGAAGTA
    TTAGATGGTACATCTTTCAATGAGTTTGTAGTCAAATTTGACAAACCAATTCAACAAGTT
    AACGAGGAATTAGTGAAGTACAATATTATCGGTGGTTTTGATTTAGGTGTTGTTTCAGAC
    GACTTTAAAAATCACATGTTAATAGCTGTAACTGAATTAAGAACAAAAGATGAAATCGAT
    ACATTTGTAGAAAAGGCTGGTGAGTTAAATGACTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1356

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1440 [new locus tag: NWMN_RS08125 ]
  • symbol: GcvPA
  • description: glycine dehydrogenase subunit 1
  • length: 451
  • theoretical pI: 4.83131
  • theoretical MW: 50031.5
  • GRAVY: -0.175166

Function[edit source | edit]

  • reaction:
    EC 1.4.4.2?  ExPASy
    Glycine dehydrogenase (aminomethyl-transferring)Glycine + [glycine-cleavage complex H protein]-N6-lipoyl-L-lysine = [glycine-cleavage complex H protein]-S-aminomethyl-N6-dihydrolipoyl-L-lysine + CO2
  • TIGRFAM:
    MetabolismEnergy metabolismAmino acids and aminesglycine dehydrogenase (TIGR00461; EC 1.4.4.2; HMM-score: 316.7)
    cysteine desulfurase NifS (TIGR03402; EC 2.8.1.7; HMM-score: 27.2)
    Genetic information processingProtein synthesistRNA and rRNA base modificationcysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 25.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOthercysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 25.2)
    Unknown functionEnzymes of unknown specificitycysteine desulfurase family protein (TIGR01977; HMM-score: 25)
    Genetic information processingDNA metabolismRestriction/modificationcysteine desulfurase DndA (TIGR03235; EC 2.8.1.7; HMM-score: 19.9)
    MetabolismEnergy metabolismAmino acids and aminestyrosine aminotransferase (TIGR01264; EC 2.6.1.5; HMM-score: 14.6)
    tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 12)
    cysteine desulfurase, NifS family (TIGR03403; EC 2.8.1.7; HMM-score: 11.9)
  • TheSEED:  
    Amino Acids and DerivativesAlanine, serine, and glycineGlycine and Serine Utilization Glycine dehydrogenase [decarboxylating] (glycine cleavage system P1 protein) (EC 1.4.4.2) 
    Alanine, serine, and glycineGlycine cleavage system Glycine dehydrogenase [decarboxylating] (glycine cleavage system P1 protein) (EC 1.4.4.2) 
  • PFAM:
    PLP_aminotran (CL0061) GDC-P; Glycine cleavage system P-protein (PF02347; HMM-score: 506.5)
    Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 22.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.131
    • Ymax_pos: 26
    • Cmax: 0.126
    • Cmax_pos: 26
    • Smax: 0.165
    • Smax_pos: 16
    • Smean: 0.13
    • D: 0.131
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MCIVSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNIAEGEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTPYQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVVSKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDETAAVAVQYPNFYGSIEDLEKIHSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGGPHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQALNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDKPIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]