NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1456 [new locus tag: NWMN_RS08215 ]
• pan locus tag^?: SAUPAN004134000
• symbol: sodA
• pan gene symbol^?: sodA
• synonym:
• product: superoxide dismutase Mn/Fe family protein

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1456 [new locus tag: NWMN_RS08215 ]
• symbol: sodA
• product: superoxide dismutase Mn/Fe family protein
• replicon: chromosome
• strand: -
• coordinates: 1623938..1624537
• length: 600
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332245 NCBI
• RefSeq: YP_001332490 NCBI
• BioCyc:
• MicrobesOnline: 3707008 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGGCTTTTGAATTACCAAAATTACCATACGCATTTGATGCATTAGAACCACATTTTGAC
  AAAGAAACTATGGAAATTCACCATGACAGACATCATAACACGTATGTTACGAAATTAAAT
  GCTGCAGTAGAAGGTACAGATTTAGAATCTAAATCTATTGAAGAAATTGTTGCTAATTTA
  GACAGTGTACCAGCTAACATCCAAACTGCTGTACGTAATAATGGCGGTGGACATTTAAAC
  CATTCATTATTCTGGGAGTTACTTTCACCAAACTCAGAAGAAAAAGGTACTGTAGTAGAA
  AAAATTAAAGAACAATGGGGTTCTTTAGAAGAATTTAAAAAAGAATTTGCTGACAAAGCA
  GCTGCACGCTTTGGTTCAGGTTGGGCTTGGTTAGTCGTAAACAATGGCCAGTTAGAAATT
  GTGACTACACCAAACCAAGATAATCCATTAACTGAGGGTAAAACACCTATTTTAGGTTTA
  GACGTATGGGAACACGCTTATTACCTAAAATATCAAAACAAACGCCCTGACTACATTGGC
  GCATTTTGGAATGTAGTTAACTGGGAAAAAGTTGACGAATTATATAATGCAACAAAATAA
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⊟Protein

⊟General

• locus tag: NWMN_1456 [new locus tag: NWMN_RS08215 ]
• symbol: SodA
• description: superoxide dismutase Mn/Fe family protein
• length: 199
• theoretical pI: 4.8565
• theoretical MW: 22711.2
• GRAVY: -0.575879

⊟Function

• reaction:
  EC 1.15.1.1?  ExPASy
  Superoxide dismutase2 superoxide + 2 H⁺ = O₂ + H₂O₂
• TIGRFAM:
• TheSEED:  
  Stress ResponseOxidative stressOxidative stress Superoxide dismutase [Fe] (EC 1.15.1.1) 
  and 3 more
  Oxidative stressOxidative stress Superoxide dismutase [Mn] (EC 1.15.1.1) 
  Oxidative stressProtection from Reactive Oxygen Species Superoxide dismutase [Fe] (EC 1.15.1.1) 
  Nitric oxide synthase Superoxide dismutase [Fe] (EC 1.15.1.1) 
• PFAM:
  no clan definedSod_Fe_C; Iron/manganese superoxide dismutases, C-terminal domain (PF02777; HMM-score: 144.2)
  Sod_Fe_N; Iron/manganese superoxide dismutases, alpha-hairpin domain (PF00081; HMM-score: 119.3)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0.01
  • Cytoplasmic Membrane Score: 0.09
  • Cellwall Score: 0.18
  • Extracellular Score: 9.72
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular Possibility: 1
  • Signal Peptide Possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • Ymax: 0.106
  • Ymax_pos: 31
  • Cmax: 0.105
  • Cmax_pos: 53
  • Smax: 0.158
  • Smax_pos: 20
  • Smean: 0.094
  • D: 0.101
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221668 NCBI
• RefSeq: YP_001332490 NCBI
• UniProt: A0A0H3K8D3 UniProt

⊟Protein sequence

• MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVEGTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKIKEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGLDVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: sodA no polycistronic organisation predicted

⊟Regulation

• sigma factor:
• regulator: CodY (repression) regulon

  CodY

  (TF)

  important in Amino acid metabolism;

  RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Other Information

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