NCBI: 06-JUL-2013
Contents
⊟Summary[edit source | edit]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_1456 [new locus tag: NWMN_RS08215 ]
- pan locus tag?: SAUPAN004134000
- symbol: sodA
- pan gene symbol?: sodA
- synonym:
- product: superoxide dismutase Mn/Fe family protein
⊟Genome View[edit source | edit]
⊟Gene[edit source | edit]
⊟General[edit source | edit]
- type: CDS
- locus tag: NWMN_1456 [new locus tag: NWMN_RS08215 ]
- symbol: sodA
- product: superoxide dismutase Mn/Fe family protein
- replicon: chromosome
- strand: -
- coordinates: 1623938..1624537
- length: 600
- essential: unknown other strains
⊟Accession numbers[edit source | edit]
- Gene ID: 5332245 NCBI
- RefSeq: YP_001332490 NCBI
- BioCyc:
- MicrobesOnline: 3707008 MicrobesOnline
⊟Phenotype[edit source | edit]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit source | edit]
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541ATGGCTTTTGAATTACCAAAATTACCATACGCATTTGATGCATTAGAACCACATTTTGAC
AAAGAAACTATGGAAATTCACCATGACAGACATCATAACACGTATGTTACGAAATTAAAT
GCTGCAGTAGAAGGTACAGATTTAGAATCTAAATCTATTGAAGAAATTGTTGCTAATTTA
GACAGTGTACCAGCTAACATCCAAACTGCTGTACGTAATAATGGCGGTGGACATTTAAAC
CATTCATTATTCTGGGAGTTACTTTCACCAAACTCAGAAGAAAAAGGTACTGTAGTAGAA
AAAATTAAAGAACAATGGGGTTCTTTAGAAGAATTTAAAAAAGAATTTGCTGACAAAGCA
GCTGCACGCTTTGGTTCAGGTTGGGCTTGGTTAGTCGTAAACAATGGCCAGTTAGAAATT
GTGACTACACCAAACCAAGATAATCCATTAACTGAGGGTAAAACACCTATTTTAGGTTTA
GACGTATGGGAACACGCTTATTACCTAAAATATCAAAACAAACGCCCTGACTACATTGGC
GCATTTTGGAATGTAGTTAACTGGGAAAAAGTTGACGAATTATATAATGCAACAAAATAA60
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⊟Protein[edit source | edit]
⊟General[edit source | edit]
- locus tag: NWMN_1456 [new locus tag: NWMN_RS08215 ]
- symbol: SodA
- description: superoxide dismutase Mn/Fe family protein
- length: 199
- theoretical pI: 4.8565
- theoretical MW: 22711.2
- GRAVY: -0.575879
⊟Function[edit source | edit]
- reaction: EC 1.15.1.1? ExPASySuperoxide dismutase2 superoxide + 2 H+ = O2 + H2O2
- TIGRFAM:
- TheSEED: Nitrogen MetabolismNitrogen Metabolism - no subcategoryNitric oxide synthase Manganese superoxide dismutase (EC 1.15.1.1)and 6 moreProtein MetabolismProtein biosynthesisProlyl-tRNA synthetase associated editing enzymes Superoxide dismutase [Fe] (EC 1.15.1.1)Superoxide dismutase [Fe] (EC 1.15.1.1)Superoxide dismutase [Mn] (EC 1.15.1.1)Superoxide dismutase [Fe] (EC 1.15.1.1)
- PFAM: no clan definedSod_Fe_C; Iron/manganese superoxide dismutases, C-terminal domain (PF02777; HMM-score: 144.2)Sod_Fe_N; Iron/manganese superoxide dismutases, alpha-hairpin domain (PF00081; HMM-score: 119.3)
⊟Structure, modifications & interactions[edit source | edit]
- domains:
- modifications:
- cofactors:
- effectors:
- protein partners:
⊟Localization[edit source | edit]
- PSORTb: Extracellular
- Cytoplasmic Score: 0.01
- Cytoplasmic Membrane Score: 0.09
- Cellwall Score: 0.18
- Extracellular Score: 9.72
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular Possibility: 1
- Signal Peptide Possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- Ymax: 0.106
- Ymax_pos: 31
- Cmax: 0.105
- Cmax_pos: 53
- Smax: 0.158
- Smax_pos: 20
- Smean: 0.094
- D: 0.101
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit source | edit]
⊟Protein sequence[edit source | edit]
- MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVEGTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKIKEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGLDVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK
⊟Experimental data[edit source | edit]
- experimentally validated: no data available
⊟Expression & Regulation[edit source | edit]
⊟Operon[edit source | edit]
- MicrobesOnline: sodA no polycistronic organisation predicted
⊟Regulation[edit source | edit]
- regulator: CodY (repression) regulon
CodY (TF) important in Amino acid metabolism; RegPrecise
⊟Transcription pattern[edit source | edit]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit source | edit]
⊟Protein stability[edit source | edit]
- half-life: no data available
⊟Biological Material[edit source | edit]
⊟Mutants[edit source | edit]
⊟Expression vector[edit source | edit]
⊟lacZ fusion[edit source | edit]
⊟GFP fusion[edit source | edit]
⊟two-hybrid system[edit source | edit]
⊟FLAG-tag construct[edit source | edit]
⊟Antibody[edit source | edit]
⊟Other Information[edit source | edit]
You are kindly invited to share additional interesting facts.
⊟Literature[edit source | edit]
⊟References[edit source | edit]
⊟Relevant publications[edit source | edit]