NCBI: 06-JUL-2013
Contents
⊟Summary[edit source | edit]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_1470 [new locus tag: NWMN_RS08285 ]
- pan locus tag?: SAUPAN004148000
- symbol: era
- pan gene symbol?: era
- synonym:
- product: GTP-binding protein Era
⊟Genome View[edit source | edit]
⊟Gene[edit source | edit]
⊟General[edit source | edit]
- type: CDS
- locus tag: NWMN_1470 [new locus tag: NWMN_RS08285 ]
- symbol: era
- product: GTP-binding protein Era
- replicon: chromosome
- strand: -
- coordinates: 1638589..1639488
- length: 900
- essential: unknown other strains
⊟Accession numbers[edit source | edit]
- Gene ID: 5332343 NCBI
- RefSeq: YP_001332504 NCBI
- MicrobesOnline: 3707022 MicrobesOnline
⊟Phenotype[edit source | edit]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit source | edit]
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841ATGACAGAACATAAATCAGGATTTGTTTCAATTATAGGTAGACCAAATGTAGGAAAGTCA
ACATTTGTTAATAGAGTGATCGGCCATAAAATAGCAATCATGTCCGATAAAGCTCAAACA
ACTAGAAATAAAATTCAAGGTGTTATGACAAGAGATGACGCGCAAATTATATTCATTGAT
ACGCCAGGTATTCATAAACCTAAACACAAATTAGGTGACTATATGATGAAAGTCGCTAAA
AATACATTATCTGAGATAGATGCAATCATGTTTATGGTTAATGCCAATGAGGAAATTGGA
CGAGGCGATGAATATATTATAGAAATGTTGAAAAATGTTAAGACACCAGTATTTTTAGTA
TTAAATAAAATAGATTTAGTGCATCCAGATGAATTAATGCCAAAGATTGAAGAATATCAA
AGTTATATGGACTTTACAGAGATTGTACCTATTTCAGCATTAGAAGGGCTAAATGTCGAT
CATTTTATTGATGTTTTAAAGACGTATTTACCCGAAGGACCTAAATATTATCCAGATGAT
CAAATTTCAGACCATCCTGAACAATTTGTAGTGGGTGAAATCATTCGTGAAAAAATCCTT
CATCTTACAAGTGAAGAAATCCCTCATGCGATTGGTGTTAATGTGGACCGTATGGTTAAA
GAAAGCGAAGATCGTGTTCATATCGAAGCAACTATATATGTTGAAAGAGATTCGCAAAAA
GGAATTGTCATTGGAAAAGGCGGTAAAAAGTTAAAAGAAGTAGGAAAACGTGCGAGACGT
GATATAGAAATGCTTCTAGGCTCTAAAGTTTACTTAGAATTATGGGTCAAAGTTCAAAGA
GACTGGCGAAACAAAGTTAACTTTATTCGCCAAATTGGTTATGTTGAAGACCAAGATTAA60
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⊟Protein[edit source | edit]
⊟General[edit source | edit]
- locus tag: NWMN_1470 [new locus tag: NWMN_RS08285 ]
- symbol: Era
- description: GTP-binding protein Era
- length: 299
- theoretical pI: 6.5234
- theoretical MW: 34328.5
- GRAVY: -0.353846
⊟Function[edit source | edit]
- reaction:
- TIGRFAM: Protein synthesisOtherGTP-binding protein Era (TIGR00436; HMM-score: 351)Protein synthesisOtherribosome-associated GTPase EngA (TIGR03594; HMM-score: 133.5)Unknown functionGeneralsmall GTP-binding protein domain (TIGR00231; HMM-score: 96.4)Protein fateProtein modification and repair[FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 79.4)Protein synthesistRNA and rRNA base modificationtRNA modification GTPase TrmE (TIGR00450; EC 3.6.-.-; HMM-score: 71.4)Protein synthesisOtherribosome biogenesis GTP-binding protein YlqF (TIGR03596; HMM-score: 57.9)Protein synthesisOtherribosome biogenesis GTP-binding protein YsxC (TIGR03598; HMM-score: 54.3)Transport and binding proteinsCations and iron carrying compoundsferrous iron transport protein B (TIGR00437; HMM-score: 41.5)Protein synthesisOtherObg family GTPase CgtA (TIGR02729; HMM-score: 41.2)Unknown functionGeneralGTP-binding protein HflX (TIGR03156; HMM-score: 40.1)Protein synthesisOtherribosome biogenesis GTPase YqeH (TIGR03597; HMM-score: 37.5)Protein synthesisTranslation factorstranslation initiation factor IF-2 (TIGR00487; HMM-score: 35.1)translation initiation factor 2, gamma subunit (TIGR03680; HMM-score: 34.2)Transport and binding proteinsNucleosides, purines and pyrimidinesGTP-binding protein (TIGR00991; HMM-score: 20.7)Protein synthesisTranslation factorstranslation initiation factor aIF-2 (TIGR00491; HMM-score: 19.8)Protein synthesisTranslation factorsribosome small subunit-dependent GTPase A (TIGR00157; EC 3.6.-.-; HMM-score: 15.3)Transport and binding proteinsAmino acids, peptides and amineschloroplast protein import component Toc86/159, G and M domains (TIGR00993; HMM-score: 15.1)nicotinamide-nucleotide adenylyltransferase (TIGR01526; EC 2.7.7.1; HMM-score: 13.5)Protein synthesisTranslation factorspeptide chain release factor 3 (TIGR00503; HMM-score: 13.4)
- TheSEED:
- PFAM: P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 86.7)KH (CL0007) KH_2; KH domain (PF07650; HMM-score: 81.9)P-loop_NTPase (CL0023) FeoB_N; Ferrous iron transport protein B (PF02421; HMM-score: 50.1)GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 48)Dynamin_N; Dynamin family (PF00350; HMM-score: 45.2)AIG1; AIG1 family (PF04548; HMM-score: 32.3)RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 29.1)PduV-EutP; Ethanolamine utilisation - propanediol utilisation (PF10662; HMM-score: 23.4)cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 21.9)no clan definedMMR_HSR1_Xtn; C-terminal region of MMR_HSR1 domain (PF16897; HMM-score: 19.9)P-loop_NTPase (CL0023) Ras; Ras family (PF00071; HMM-score: 19.3)Roc; Ras of Complex, Roc, domain of DAPkinase (PF08477; HMM-score: 16.7)Gtr1_RagA; Gtr1/RagA G protein conserved region (PF04670; HMM-score: 14.5)ATP_bind_1; Conserved hypothetical ATP binding protein (PF03029; HMM-score: 14.1)MMR_HSR1_C; GTPase of unknown function C-terminal (PF08438; HMM-score: 13.2)KH (CL0007) KH_4; KH domain (PF13083; HMM-score: 12.8)P-loop_NTPase (CL0023) AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 12.6)KH (CL0007) KH_1; KH domain (PF00013; HMM-score: 12.5)
⊟Structure, modifications & interactions[edit source | edit]
- domains:
- modifications:
- cofactors:
- effectors:
- protein partners:
NWMN_1625 (fhs) formate--tetrahydrofolate ligase [1] (data from MRSA252) NWMN_0741 (gapA) glyceraldehyde 3-phosphate dehydrogenase 1 [1] (data from MRSA252) NWMN_1103 (ileS) isoleucyl-tRNA synthetase [1] (data from MRSA252) NWMN_1176 (nusA) transcription elongation factor NusA [1] (data from MRSA252) NWMN_0501 (rplJ) 50S ribosomal protein L10 [1] (data from MRSA252) NWMN_2146 (rpsC) 30S ribosomal protein S3 [1] (data from MRSA252) NWMN_0641 hypothetical protein [1] (data from MRSA252) NWMN_0735 hypothetical protein [1] (data from MRSA252) NWMN_0949 phosphocarrier protein HPr [1] (data from MRSA252)
⊟Localization[edit source | edit]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 1.05
- Cytoplasmic Membrane Score: 8.78
- Cellwall Score: 0.08
- Extracellular Score: 0.09
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular Possibility: 1
- Signal Peptide Possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- Ymax: 0.145
- Ymax_pos: 35
- Cmax: 0.112
- Cmax_pos: 39
- Smax: 0.294
- Smax_pos: 30
- Smean: 0.163
- D: 0.152
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit source | edit]
⊟Protein sequence[edit source | edit]
- MTEHKSGFVSIIGRPNVGKSTFVNRVIGHKIAIMSDKAQTTRNKIQGVMTRDDAQIIFIDTPGIHKPKHKLGDYMMKVAKNTLSEIDAIMFMVNANEEIGRGDEYIIEMLKNVKTPVFLVLNKIDLVHPDELMPKIEEYQSYMDFTEIVPISALEGLNVDHFIDVLKTYLPEGPKYYPDDQISDHPEQFVVGEIIREKILHLTSEEIPHAIGVNVDRMVKESEDRVHIEATIYVERDSQKGIVIGKGGKKLKEVGKRARRDIEMLLGSKVYLELWVKVQRDWRNKVNFIRQIGYVEDQD
⊟Experimental data[edit source | edit]
- experimentally validated: no data available
⊟Expression & Regulation[edit source | edit]
⊟Operon[edit source | edit]
⊟Regulation[edit source | edit]
- sigma factor:
- regulator:
⊟Transcription pattern[edit source | edit]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit source | edit]
⊟Protein stability[edit source | edit]
- half-life: no data available
⊟Biological Material[edit source | edit]
⊟Mutants[edit source | edit]
⊟Expression vector[edit source | edit]
⊟lacZ fusion[edit source | edit]
⊟GFP fusion[edit source | edit]
⊟two-hybrid system[edit source | edit]
⊟FLAG-tag construct[edit source | edit]
⊟Antibody[edit source | edit]
⊟Other Information[edit source | edit]
You are kindly invited to share additional interesting facts.
⊟Literature[edit source | edit]
⊟References[edit source | edit]
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8
Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J. Proteome Res.: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)
⊟Relevant publications[edit source | edit]