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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1653 [new locus tag: NWMN_RS09275 ]
  • pan locus tag?: SAUPAN004446000
  • symbol: NWMN_1653
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1653 [new locus tag: NWMN_RS09275 ]
  • symbol: NWMN_1653
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1849653..1850606
  • length: 954
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGGCAATCATTTCCAATACGCTTTTGAAAACAAACGTTATCACACATGGAATTACCAT
    TTAAAAAATAAATTTGGACAAAAAATATTTAAAGTTGCATTGGATGGCGGGTTTGACTGT
    CCTAACCGCGATGGCACTGTAGCACATGGTGGATGTACATTTTGTTCTGCTGCAGGTAGC
    GGAGACTTTGCAGGTAATCGTGCAGATTCAATCGCAGTACAATTTAAAGAAATTAAAGAA
    AAGATGCATGAGAAATGGCACGAAGGAAAATATATTGCTTATTTTCAGGCATTTACAAAT
    ACACATGCACCGGTTGAAGTATTAAAAGAAAAATTCGAACCTGTACTTAAAGAACCGGGT
    GTTGTGGGATTATCTATTGGTACGCGTCCTGACTGTCTACCAGACGATGTTGTCGAATAT
    TTAGCAGATTTGAATCAACGAACATACTTATGGGTTGAATTAGGACTACAAACAATCCAT
    CAGTCAACATCTGATTTAATCAATCGTGCCCATGATATGAAAACTTATTATGATGGTGTG
    GCAAAATTACGTAAGCATAATATCAATGTATGTACACACATCATTAATGGCTTACCTGGC
    GAAGACTATGACATGATGATGGCTACTGCCAAAGAAGTTGCACAAATGGATGTACAAGGT
    ATTAAAATTCATTTACTTCATTTGTTAAAAGGTACACCGATGGTAAAACAATACGATAAA
    GGTTTATTAACTTTTATGACTCAAGAAGAGTACACAAACCTAGTTGTGGACCAATTAGAA
    GTGATTCCCCCTGAAATGATCGTTCACCGAATTACCGGTGATGGTCCAATAGATATCATG
    GTAGGTCCAATGTGGAGTGTTAATAAATGGGAAGTATTAAATGGCATCGATGCTGAATTA
    GCACGTAGAAATTCTTATCAAGGCTTGCGTTACAAGTCTAAGGTGAAGCAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    954

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1653 [new locus tag: NWMN_RS09275 ]
  • symbol: NWMN_1653
  • description: hypothetical protein
  • length: 317
  • theoretical pI: 7.03345
  • theoretical MW: 36053.1
  • GRAVY: -0.393691

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Unknown functionEnzymes of unknown specificityradical SAM protein, TIGR01212 family (TIGR01212; HMM-score: 433.5)
    Genetic information processingTranscriptionDNA-dependent RNA polymeraseradical SAM enzyme/protein acetyltransferase, ELP3 family (TIGR01211; EC 2.3.1.48; HMM-score: 63)
    Unknown functionEnzymes of unknown specificityB12-binding domain/radical SAM domain protein, MJ_1487 family (TIGR04013; HMM-score: 39.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalamincoproporphyrinogen dehydrogenase HemZ (TIGR03994; EC 1.3.99.22; HMM-score: 38.8)
    putative heme utilization radical SAM enzyme HutW (TIGR04107; HMM-score: 32)
    radical SAM methylthiotransferase, MiaB/RimO family (TIGR00089; EC 2.1.1.-,2.8.1.-; HMM-score: 28.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersBiotinbiotin synthase (TIGR00433; EC 2.8.1.6; HMM-score: 27.3)
    Hypothetical proteinsConservedradical SAM enzyme, TIGR01210 family (TIGR01210; HMM-score: 24.4)
    Unknown functionEnzymes of unknown specificityB12-binding domain/radical SAM domain protein, MJ_0865 family (TIGR04014; HMM-score: 22.9)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminoxygen-independent coproporphyrinogen III oxidase (TIGR00538; EC 1.3.99.22; HMM-score: 22.8)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA-i(6)A37 thiotransferase enzyme MiaB (TIGR01574; EC 2.-.-.-; HMM-score: 19.6)
    Cellular processesCellular processesToxin production and resistanceradical SAM P-methyltransferase, PhpK family (TIGR04479; EC 2.1.-.-; HMM-score: 18.3)
    Genetic information processingProtein synthesistRNA and rRNA base modificationMiaB-like tRNA modifying enzyme (TIGR01579; HMM-score: 17.3)
    Genetic information processingProtein synthesistRNA and rRNA base modificationMiaB-like tRNA modifying enzyme, archaeal-type (TIGR01578; HMM-score: 15.1)
    hopanoid biosynthesis associated radical SAM protein HpnJ (TIGR03471; HMM-score: 14.7)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminputative oxygen-independent coproporphyrinogen III oxidase (TIGR00539; EC 1.3.99.22; HMM-score: 12.3)
  • TheSEED:  
    Uncharacterized radical SAM protein YtqA 
  • PFAM:
    no clan definedRadical_SAM_C; Radical_SAM C-terminal domain (PF16199; HMM-score: 82.9)
    TIM_barrel (CL0036) Radical_SAM; Radical SAM superfamily (PF04055; HMM-score: 52.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.126
    • Ymax_pos: 38
    • Cmax: 0.104
    • Cmax_pos: 65
    • Smax: 0.21
    • Smax_pos: 25
    • Smean: 0.141
    • D: 0.132
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRDGTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTHAPVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIHQSTSDLINRAHDMKTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDVQGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGPIDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]