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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1967 [new locus tag: NWMN_RS11350 ]
  • pan locus tag?: SAUPAN005311000
  • symbol: ilvA
  • pan gene symbol?: ilvA2
  • synonym:
  • product: threonine dehydratase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1967 [new locus tag: NWMN_RS11350 ]
  • symbol: ilvA
  • product: threonine dehydratase
  • replicon: chromosome
  • strand: +
  • coordinates: 2178912..2180180
  • length: 1269
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACAGTCAAAACAACAGTTTCTACGAAAGATATCGATGAGGCATTTTTAAGACTTAAA
    GATATTGTCAAAGAAACACCTTTACAATTAGACCATTACTTATCTCAAAAGTATGATTGT
    AAAGTCTATTTAAAACGAGAAGATTTACAATGGGTACGTTCTTTTAAATTAAGAGGTGCT
    TACAACGCTATTTCTGTTTTATCAGATGAAGCTAAAAGTAAAGGTATTACATGTGCAAGT
    GCAGGTAATCATGCTCAAGGTGTTGCCTATACAGCTAAAAAACTTAATTTAAACGCTGTT
    ATCTTTATGCCAGTCACTACACCTTTACAAAAGGTAAATCAAGTAAAGTTCTTTGGAAAT
    AGTAACGTTGAAGTTGTACTCACTGGTGATACATTTGATCACTGTTTAGCTGAAGCTTTA
    ACTTATACAAGTGAACATCAAATGAACTTTATAGATCCATTCAATAATGTTCATACAATT
    TCTGGACAAGGTACGCTTGCTAAAGAAATGCTAGAACAAGCAAAGTCTGACAATGTTAAC
    TTTGATTATCTATTTGCCGCAATTGGTGGTGGCGGTTTAATTTCAGGTATTAGTACTTAC
    TTTAAAACCTATTCACCTACCACGAAAATTATAGGTGTTGAACCTTCAGGTGCAAGTAGT
    ATGTATGAATCTGTTGTGGTAAATAATCAGGTAGTCACATTGCCTAATATCGATAAATTT
    GTGGACGGTGCATCTGTAGCTAGAGTTGGCGATATTACATTTGAAATTGCAAAAGAAAAT
    GTAGATGATTACGTTCAAGTAGATGAAGGTGCAGTTTGTTCTACGATTTTAGATATGTAT
    TCAAAACAAGCAATTGTAGCAGAACCTGCTGGCGCATTAAGTGTAAGTGCGCTTGAAAAC
    TATAAAGATCATATTAAAGGTAAAACAGTGGTTTGTGTCATTAGTGGTGGTAATAATGAT
    ATTAATCGAATGAAAGAAATTGAAGAACGTTCATTACTATACGAAGAAATGAAGCATTAC
    TTTATCTTAAATTTCCCTCAACGTCCAGGTGCATTGAGAGAATTTGTAAATGACGTATTA
    GGACCTCAAGACGATATTACTAAATTTGAATACTTAAAAAAATCTTCTCAAAATACAGGT
    ACTGTCATTATTGGTATTCAACTTAAAGATCATGATGATTTAATACAACTCAAACAACGT
    GTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTG
    TTAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1269

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1967 [new locus tag: NWMN_RS11350 ]
  • symbol: IlvA
  • description: threonine dehydratase
  • length: 422
  • theoretical pI: 5.46176
  • theoretical MW: 46937.1
  • GRAVY: -0.182938

Function[edit source | edit]

  • reaction:
    EC 4.3.1.19?  ExPASy
    Threonine ammonia-lyaseL-threonine = 2-oxobutanoate + NH3
  • TIGRFAM:
    MetabolismAmino acid biosynthesisPyruvate familythreonine dehydratase (TIGR02079; EC 4.3.1.19; HMM-score: 615.2)
    MetabolismAmino acid biosynthesisPyruvate familythreonine ammonia-lyase, biosynthetic (TIGR01124; EC 4.3.1.19; HMM-score: 361.8)
    MetabolismAmino acid biosynthesisPyruvate familythreonine ammonia-lyase (TIGR01127; EC 4.3.1.19; HMM-score: 309.1)
    ectoine utilization protein EutB (TIGR02991; HMM-score: 194.2)
    MetabolismAmino acid biosynthesisSerine familycysteine synthase (TIGR01136; EC 2.5.1.47; HMM-score: 65.3)
    MetabolismAmino acid biosynthesisSerine familycysteine synthase A (TIGR01139; EC 2.5.1.47; HMM-score: 63.4)
    Cellular processesCellular processesBiosynthesis of natural products2,3-diaminopropionate biosynthesis protein SbnA (TIGR03945; HMM-score: 63.1)
    MetabolismAmino acid biosynthesisAspartate familythreonine synthase (TIGR00260; EC 4.2.3.1; HMM-score: 60.5)
    diaminopropionate ammonia-lyase (TIGR03528; EC 4.3.1.15; HMM-score: 59.4)
    MetabolismEnergy metabolismOtherdiaminopropionate ammonia-lyase family (TIGR01747; EC 4.3.1.15; HMM-score: 57.3)
    MetabolismAmino acid biosynthesisAromatic amino acid familytryptophan synthase, beta subunit (TIGR00263; EC 4.2.1.20; HMM-score: 51.3)
    pyridoxal phosphate-dependent enzymes, D-cysteine desulfhydrase family (TIGR01275; HMM-score: 48.1)
    MetabolismAmino acid biosynthesisSerine familycystathionine beta-synthase (TIGR01137; EC 4.2.1.22; HMM-score: 43.1)
    MetabolismAmino acid biosynthesisSerine familycysteine synthase B (TIGR01138; EC 2.5.1.47; HMM-score: 38.8)
    Unknown functionEnzymes of unknown specificitypyridoxal-phosphate dependent TrpB-like enzyme (TIGR01415; HMM-score: 17.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOthercysteate synthase (TIGR03844; EC 2.5.1.76; HMM-score: 16.1)
    MetabolismEnergy metabolismMethanogenesiscysteate synthase (TIGR03844; EC 2.5.1.76; HMM-score: 16.1)
  • TheSEED:  
    Amino Acids and DerivativesBranched-chain amino acidsBranched-Chain Amino Acid Biosynthesis Threonine dehydratase biosynthetic (EC 4.3.1.19) 
  • PFAM:
    no clan definedPALP; Pyridoxal-phosphate dependent enzyme (PF00291; HMM-score: 246.1)
    ACT (CL0070) Thr_dehydrat_C; C-terminal regulatory domain of Threonine dehydratase (PF00585; HMM-score: 75.1)
    TIM_barrel (CL0036) SOR_SNZ; SOR/SNZ family (PF01680; HMM-score: 14.2)
    PP-binding (CL0314) Ribosomal_L50; Ribosomal subunit 39S (PF10501; HMM-score: 12.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.102
    • Ymax_pos: 13
    • Cmax: 0.105
    • Cmax_pos: 26
    • Smax: 0.12
    • Smax_pos: 9
    • Smean: 0.104
    • D: 0.103
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYLKREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIFMPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTISGQGTLAKEMLEQAKSDNVNFDYLFAAIGGGGLISGISTYFKTYSPTTKIIGVEPSGASSMYESVVVNNQVVTLPNIDKFVDGASVARVGDITFEIAKENVDDYVQVDEGAVCSTILDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNNDINRMKEIEERSLLYEEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHDDLIQLKQRVNHFDPSNIYINENKMLYSLLI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CodY
    CodY (TF) important in Amino acid metabolism

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]