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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2318 [new locus tag: NWMN_RS13345 ]
  • pan locus tag?: SAUPAN005958000
  • symbol: hlgA
  • pan gene symbol?: hlgA
  • synonym:
  • product: gamma-hemolysin component A

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2318 [new locus tag: NWMN_RS13345 ]
  • symbol: hlgA
  • product: gamma-hemolysin component A
  • replicon: chromosome
  • strand: +
  • coordinates: 2547061..2547990
  • length: 930
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGATTAAAAATAAAATATTAACAGCAACTTTAGCAGTTGGTTTAATAGCCCCTTTAGCC
    AATCCATTTATAGAAATTTCTAAAGCAGAAAATAAGATAGAAGATATCGGCCAAGGTGCA
    GAAATCATCAAAAGAACACAAGACATTACTAGCAAACGATTAGCTATAACTCAAAACATT
    CAATTTGATTTTGTAAAAGATAAAAAATATAACAAAGATGCCCTAGTTGTTAAGATGCAA
    GGCTTCATTAGCTCTAGAACAACATATTCAGACTTAAAAAAATATCCATATATTAAAAGA
    ATGATATGGCCATTTCAATATAATATCAGTTTGAAAACGAAAGACTCTAATGTTGATTTA
    ATTAATTATCTTCCTAAAAATAAAATTGATTCAGCAGATGTTAGTCAGAAATTAGGCTAT
    AATATCGGCGGAAACTTCCAATCAGCGCCATCAATCGGAGGCAGTGGCTCATTCAACTAC
    TCTAAAACAATTAGTTATAATCAAAAAAACTATGTTACTGAAGTAGAAAGTCAGAACTCT
    AAAGGTGTTAAATGGGGAGTGAAAGCAAATTCATTCGTTACACCGAATGGTCAAGTATCT
    GCATATGATCAATACTTATTTGCACAAGACCCAACTGGTCCAGCAGCACGAGACTATTTC
    GTCCCAGATAATCAACTACCTCCTTTAATTCAAAGTGGCTTTAATCCATCATTTATTACA
    ACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGA
    AACATGGATGCTACATATGCTTACGTGACAAGACATCGTTTAGCCGTTGATAGAAAACAT
    GATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAA
    GTAAAAATTAAAAGCATCACACCTAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    930

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_2318 [new locus tag: NWMN_RS13345 ]
  • symbol: HlgA
  • description: gamma-hemolysin component A
  • length: 309
  • theoretical pI: 10.0999
  • theoretical MW: 34955.4
  • GRAVY: -0.564725

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cellular processesCellular processesToxin production and resistancebeta-channel forming cytolysin (TIGR01002; HMM-score: 291.6)
  • TheSEED:  
    Virulence, Disease and DefenseToxins and superantigensStaphylococcus Two-component and Pore-forming Cytolysins Gamma-hemolysin component A 
  • PFAM:
    Leukocidin (CL0636) Leukocidin; Leukocidin/Hemolysin toxin family (PF07968; HMM-score: 286.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 10
    • Internal Helices: 0
  • LocateP: N-terminally anchored (with CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: -0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: EISKAENK
  • SignalP: Signal peptide length 30 aa
    • Ymax: 0.555
    • Ymax_pos: 30
    • Cmax: 0.591
    • Cmax_pos: 30
    • Smax: 0.737
    • Smax_pos: 6
    • Smean: 0.597
    • D: 0.572
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MIKNKILTATLAVGLIAPLANPFIEISKAENKIEDIGQGAEIIKRTQDITSKRLAITQNIQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMIWPFQYNISLKTKDSNVDLINYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNYSKTISYNQKNYVTEVESQNSKGVKWGVKANSFVTPNGQVSAYDQYLFAQDPTGPAARDYFVPDNQLPPLIQSGFNPSFITTLSHERGKGDKSEFEITYGRNMDATYAYVTRHRLAVDRKHDAFKNRNVTVKYEVNWKTHEVKIKSITPK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]