From AureoWiki
Jump to: navigation, search
NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2401 [new locus tag: NWMN_RS13810 ]
  • pan locus tag?: SAUPAN006130000
  • symbol: gntP
  • pan gene symbol?: gntP
  • synonym:
  • product: gluconate permease

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2401 [new locus tag: NWMN_RS13810 ]
  • symbol: gntP
  • product: gluconate permease
  • replicon: chromosome
  • strand: -
  • coordinates: 2642311..2643669
  • length: 1359
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTTTAACGAAATATGGCCGTTAATCAGTGTTGTTTTAGGTATCGTTATTTTATTAGTA
    TTAATTATTGGGTTTAAATTAAATACATTTATTTCATTAATTATCACATCGATGATTACA
    GCGTTAATGCTAGGTATACCATTGACTAAAATTATGGAGACGATTGAGAAAGGAATGGGC
    AGTACGCTCGGCCACATTGCACTGATATTCGGTTTAGGTGCCATACTTGGGAAATTGCTT
    GCTGATGGTGGTGGTGCAACTCGAATCGCAGACACGTTGATTCAAAAATTTGGTCAAAAA
    CATGTACAATGGGCAATGCTTGTTGCAGCATTTATCGTCGGTATTGCATTGTTCTTTGAA
    GTAGGTTTAGTCTTACTGATTCCATTAGTATTTACAGTAGCAAAACGCGCAAATGTTTCA
    GTGTTAAAACTAGGACTACCTATGGTAACAGCTTTATCAGTGACACATGGCTTTTTACCA
    CCACATCCAGGACCGGTAGTCATCGCAAAAGAATTAAAGGCAAATGTTGGAGATGTATTA
    CTGTATGGTATGATTATTGCCATTCCAGTTACACTCATTGCAGGGCCTATATTTAACAAA
    GTTGCACAAAAAATGATTCCGTCTGCGTATACACGAGAAGGCGATATTTCAGCGTTAGGT
    GCACAAAAAGAATTTACGGATCAAGAGATGCCAGGATTTGGTATGAGTTTATTAACAGCA
    ACATTACCAGTCATATTAATGTTAGTGTCTACGATAACGCAACTTGTAACTGGGCATGAC
    AAACCTACAAATCTATTTGAATCTATCATTTATATGATAGGAACAGCAGGGACAGCCATG
    CTAATAGCAGTATTATTTGCAATCGTTACGATGGGATTAATGAGAAAGCGTAAGATGAAT
    CATATTATGGAATCAGTGACGAATGCGATTTATCCAATCGGAATGATGTTATTGATTATT
    GGCGGTGGCGGTACATTTAAACAAGTATTAATTGACGGCGGTGTCGGAAATACAATTGCT
    AAAATGTTTGAAGGTACAGAGATGTCTCCGATTTTACTAGCATGGATTGTTGCAGCTGTG
    CTACGTATCGCATTAGGTTCGGCTACAGTAGCTGCGATTTCAACTACAGGTATTGTCTTA
    CCATTATTACAATCATCAGATGTAAATGTTGCATTAGTTGTACTTGCGATAGGTGCAGGT
    AGTGTGATTTTGTCTCATGTAAATGACGCAGGATTTTGGATGTTTAAAGAATATTTTGGT
    TTAACTGTAAAAGAAACATTCTTAACATGGTCATTATTAGAAACGATTATTTCCGTTTCA
    GGTATCATCTTCATTTTATTCATTAGCTTATTTGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1359

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_2401 [new locus tag: NWMN_RS13810 ]
  • symbol: GntP
  • description: gluconate permease
  • length: 452
  • theoretical pI: 9.2587
  • theoretical MW: 48102.9
  • GRAVY: 1.02942

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidstransporter, gluconate:H+ symporter (GntP) family (TIGR00791; HMM-score: 600.4)
  • TheSEED:  
    CarbohydratesMonosaccharidesD-gluconate and ketogluconates metabolism Gluconate permease, Bsu4004 homolog 
  • PFAM:
    IT (CL0182) GntP_permease; GntP family permease (PF02447; HMM-score: 570.6)
    CitMHS; Citrate transporter (PF03600; HMM-score: 72.3)
    Na_H_antiport_2; Na+-H+ antiporter family (PF13726; HMM-score: 16.9)
    no clan definedTim17; Tim17/Tim22/Tim23/Pmp24 family (PF02466; HMM-score: 10.6)
    IT (CL0182) DctM; Tripartite ATP-independent periplasmic transporter, DctM component (PF06808; HMM-score: 7.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.101
    • Ymax_pos: 11
    • Cmax: 0.122
    • Cmax_pos: 29
    • Smax: 0.162
    • Smax_pos: 3
    • Smean: 0.099
    • D: 0.1
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MFNEIWPLISVVLGIVILLVLIIGFKLNTFISLIITSMITALMLGIPLTKIMETIEKGMGSTLGHIALIFGLGAILGKLLADGGGATRIADTLIQKFGQKHVQWAMLVAAFIVGIALFFEVGLVLLIPLVFTVAKRANVSVLKLGLPMVTALSVTHGFLPPHPGPVVIAKELKANVGDVLLYGMIIAIPVTLIAGPIFNKVAQKMIPSAYTREGDISALGAQKEFTDQEMPGFGMSLLTATLPVILMLVSTITQLVTGHDKPTNLFESIIYMIGTAGTAMLIAVLFAIVTMGLMRKRKMNHIMESVTNAIYPIGMMLLIIGGGGTFKQVLIDGGVGNTIAKMFEGTEMSPILLAWIVAAVLRIALGSATVAAISTTGIVLPLLQSSDVNVALVVLAIGAGSVILSHVNDAGFWMFKEYFGLTVKETFLTWSLLETIISVSGIIFILFISLFV

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulators: CcpA, GntR
    CcpA (TF) important in Carbon catabolism  RegPrecise    transcription unit transferred from N315 data RegPrecise
    GntR (TF) important in Gluconate utilization  RegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]