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NCBI: 06-JUL-2013

⊟Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2504 [new locus tag: NWMN_RS14370 ]
  • pan locus tag?: SAUPAN006306000
  • symbol: NWMN_2504
  • pan gene symbol?: lqo
  • synonym:
  • product: malate:quinone oxidoreductase

⊟Genome View[edit source | edit]

⊟Gene[edit source | edit]

⊟General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2504 [new locus tag: NWMN_RS14370 ]
  • symbol: NWMN_2504
  • product: malate:quinone oxidoreductase
  • replicon: chromosome
  • strand: -
  • coordinates: 2750223..2751719
  • length: 1497
  • essential: unknown other strains

⊟Accession numbers[edit source | edit]

⊟Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCTAAGTCTAATAGTAAAGACATCGTTTTAATTGGAGCCGGTGTACTTAGCACAACA
    TTTGGTTCAATGTTAAAAGAAATTGAGCCAGACTGGAATATCCACGTTTACGAACGCTTG
    GATCGTCCTGCAATCGAAAGTTCAAACGAAAGAAATAATGCTGGTACGGGTCATGCAGCA
    TTATGTGAGTTGAACTACACAGTTTTACAACCTGATGGTTCTATCGACATCGAAAAAGCG
    AAAGTGATTAACGAAGAGTTTGAGATTTCAAAACAATTCTGGGGTCACTTAGTGAAAAGC
    GGTAGCATCGAGAACCCAAGAGAATTTATCAATCCATTACCACACATCAGTTATGTTAGA
    GGTAAAAACAATGTTAAATTCTTAAAAGATCGTTACGAAGCGATGAAAGCTTTCCCTATG
    TTCGATAATATCGAATATACTGAAGACATCGAAGTAATGAAAAAATGGATTCCATTGATG
    ATGAAAGGCCGTGAAGATAACCCTGGTATCATGGCGGCAAGTAAAATTGACGAAGGTACA
    GATGTAAACTTCGGTGAATTAACACGTAAAATGGCTAAAAGCATTGAAGCACATCCAAAT
    GCTACAGTGCAATTTAACCATGAAGTTGTTGATTTTGAACAATTATCAAATGGTCAATGG
    GAAGTTACTGTTAAAAATCGCCTAACTGGTGAGAAATTCAAACAAGTAACTGACTACGTA
    TTCATCGGTGCTGGCGGTGGAGCAATTCCATTATTACAAAAAACAGGTATCCCTGAAAGT
    AAACATTTGGGTGGATTCCCTATCAGTGGTCAATTCTTAGCTTGTACAAACCCACAAGTT
    ATTGAACAACACGATGCCAAAGTTTATGGTAAAGAGCCACCTGGTACACCACCAATGACT
    GTACCTCATTTAGATACGCGTTACATTGATGGTCAAAGAACATTATTATTTGGACCATTT
    GCTAATGTTGGACCTAAATTCTTGAAAAATGGTTCTAACTTAGATTTATTCAAGTCTGTT
    AAAACATACAACATTACAACTTTATTAGCAGCAGCAGTTAAAAACTTACCTTTAATTAAA
    TACTCATTTGACCAAGTAATTATGACAAAAGAAGGTTGTATGAACCACTTACGTACTTTC
    TATCCAGAAGCACGTAATGAAGATTGGCAATTATACACTGCTGGTAAACGTGTACAAGTT
    ATCAAAGATACACCTGAACACGGTAAAGGATTCATCCAATTCGGTACAGAAGTGGTTAAC
    TCACAAGACCACACTGTAATTGCATTATTAGGTGAATCACCAGGGGCTTCAACTTCAGTT
    TCAGTTGCGTTAGAAGTATTAGAACGTAACTTCCCTGAATACAAAACTGAATGGGCACCT
    AAAATTAAGAAAATGATTCCATCATACGGTGAATCATTAATTGAAGACGAAAAATTAATG
    AGAAAAATCCGTAAACAAACTTCAAAAGACTTAGAATTAGGTTACTACGAAAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1497

⊟Protein[edit source | edit]

⊟General[edit source | edit]

  • locus tag: NWMN_2504 [new locus tag: NWMN_RS14370 ]
  • symbol: NWMN_2504
  • description: malate:quinone oxidoreductase
  • length: 498
  • theoretical pI: 6.52415
  • theoretical MW: 55998.5
  • GRAVY: -0.430924

⊟Function[edit source | edit]

  • reaction:
    EC 1.1.5.4?  ExPASy
    Malate dehydrogenase (quinone)(S)-malate + a quinone = oxaloacetate + reduced quinone
  • TIGRFAM:
    MetabolismEnergy metabolismTCA cyclemalate dehydrogenase (acceptor) (TIGR01320; EC 1.1.5.4; HMM-score: 633.5)
    and 2 more
    Cellular processesCellular processesDetoxificationCoA-disulfide reductase (TIGR03385; EC 1.8.1.14; HMM-score: 16.2)
    conserved hypothetical protein (TIGR04540; HMM-score: 12.8)
  • TheSEED:  
    CarbohydratesCentral carbohydrate metabolismTCA Cycle Malate:quinone oxidoreductase (EC 1.1.5.4) 
  • PFAM:
    NADP_Rossmann (CL0063) Mqo; Malate:quinone oxidoreductase (Mqo) (PF06039; HMM-score: 719.5)
    and 2 more
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 22.9)
    K_oxygenase; L-lysine 6-monooxygenase (NADPH-requiring) (PF13434; HMM-score: 13)

⊟Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: FAD
  • effectors:
  • protein partners:
    NWMN_1483(dnaK)molecular chaperone DnaK  [1] (data from MRSA252)
    NWMN_0509(fus)elongation factor G  [1] (data from MRSA252)
    NWMN_2446(mvaS)3-hydroxy-3-methylglutaryl coenzyme A synthase  [1] (data from MRSA252)
    NWMN_1592(pykA)pyruvate kinase  [1] (data from MRSA252)
    NWMN_0500(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    NWMN_2140(rplE)50S ribosomal protein L5  [1] (data from MRSA252)
    NWMN_0501(rplJ)50S ribosomal protein L10  [1] (data from MRSA252)
    NWMN_0499(rplK)50S ribosomal protein L11  [1] (data from MRSA252)
    NWMN_2150(rplW)50S ribosomal protein L23  [1] (data from MRSA252)
    NWMN_2135(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    NWMN_0508(rpsG)30S ribosomal protein S7  [1] (data from MRSA252)

⊟Localization[edit source | edit]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.53
    • Cellwall Score: 8.75
    • Extracellular Score: 0.7
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 0.83
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.195
    • Ymax_pos: 26
    • Cmax: 0.139
    • Cmax_pos: 60
    • Smax: 0.372
    • Smax_pos: 25
    • Smean: 0.304
    • D: 0.237
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit source | edit]

⊟Protein sequence[edit source | edit]

  • MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPAIESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGSIENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLMMKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNGQWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACTNPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNLDLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLYTAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERNFPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN

⊟Experimental data[edit source | edit]

  • experimentally validated: no data available

⊟Expression & Regulation[edit source | edit]

⊟Operon[edit source | edit]

  • NWMN_2504 no polycistronic organisation predicted

⊟Regulation[edit source | edit]

  • sigma factor:
  • regulator:

⊟Transcription pattern[edit source | edit]

⊟Protein synthesis (provided by Aureolib)[edit source | edit]

⊟Protein stability[edit source | edit]

  • half-life: no data available

⊟Biological Material[edit source | edit]

⊟Mutants[edit source | edit]

⊟Expression vector[edit source | edit]

⊟lacZ fusion[edit source | edit]

⊟GFP fusion[edit source | edit]

⊟two-hybrid system[edit source | edit]

⊟FLAG-tag construct[edit source | edit]

⊟Antibody[edit source | edit]

⊟Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

⊟Literature[edit source | edit]

⊟References[edit source | edit]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit source | edit]

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