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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2516 [new locus tag: NWMN_RS14445 ]
  • pan locus tag?: SAUPAN006323000
  • symbol: NWMN_2516
  • pan gene symbol?: citM
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2516 [new locus tag: NWMN_RS14445 ]
  • symbol: NWMN_2516
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2765595..2766965
  • length: 1371
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAATAGTGATAATATGTGGTTAACAGTAATGGGGCTCATTATTATTATTTCAATTGTA
    GGTTTACTCATTGCCAAAAAGATAAATCCAGTTGTAGGTATGACAATCATACCTTGCTTA
    GGGGCAATGATTTTAGGATATAGTGTGACAGATTTGGTTGGATTTTTTGCTAAAGGGTTA
    GATCAAGTCATCAACGTTGTTATTATGTTTATCTTTGCCATTATTTTCTTTGGCATCATG
    AACGATAGTGGTTTATTCAAGCCGCTTGTCAAACGCTTAATATTAATGACACGAGGCAAT
    GTCGTCATTGTCTGTGCAATGACAGCTTTAATTGGCACAATAGCCCAATTAGATGGGGCC
    GGTGCGGTAACATTTTTGCTTTCTATTCCTGCATTATTACCTTTATATAAAGCGTTAAAT
    ATGAATAAATATTTATTGATTTTACTATTAGCATTAAGCGCGGCGATTATGAACATGGTA
    CCTTGGGGAGGTCCAATGGCTCGTGTAGCTGCAGTGTTAAAAGCCAAAAGTGTCAATGAA
    TTATGGTATGGATTAATACCTATTCAAATAATAGGTTTCATTCTTGTTATGTTGTTTGCG
    GTATATCTTGGATTTAAAGAACAGAAACGTATCAAAAAAGCAATAGAGAGAAATGAATTA
    CCGCAAACACAAGATATAGATGTACATAAATTAGTTGAAGTATATGAACGAGATCAAGAT
    GTAAGGTTTCCTGTAAAAGGACGTGCAAGAACAAAATCATGGATAAAATGGGTGAATACA
    GCTTTAACTTTAGCTGTTATTCTATCGATGTTAATAAATATTGCGCCACCTGAATTTGCA
    TTCATGATAGGTGTTTCGTTGGCACTTGTTATTAATTTTAAATCAGTGGATGAACAAATG
    GAACGATTAAGAGCGCATGCGCCGAATGCATTAATGATGGCTGCAGTGATTATTGCAGCA
    GGTATGTTTTTAGGTGTACTAAATGAAACCGGTATGCTTAAAGCGATTGCGACCAATTTA
    ATCAAAGTGATTCCTGCAGAAGTAGGACCATACTTGCATATTATTGTAGGTTTACTTGGC
    GTACCATTAGATTTACTAACTAGCACAGACGCTTATTATTTTGCTGTGTTACCGATTGTC
    GAACAAACAGCAGGGCAATTTGGTGTACCGTCTGTATCAACAGCTTATTCAATGGTCATA
    GGGAATATTATAGGTACATTTGTCAGCCCATTTTCACCAGCCTTATGGTTGGCAATTGGT
    TTAGCAGAGGCAAACATGGGCACGTATATTAAGTATGCATTCTTTTGGATTTGGGGATTC
    GCTATCGTTATGTTAGTAATTGCAATGTTGATGGGCATTGTGACGATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1371

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_2516 [new locus tag: NWMN_RS14445 ]
  • symbol: NWMN_2516
  • description: hypothetical protein
  • length: 456
  • theoretical pI: 9.61164
  • theoretical MW: 49771.1
  • GRAVY: 0.952412

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidscitrate transporter (TIGR00784; HMM-score: 279.8)
    Cell structureCell envelopeOtherLPXTG cell wall anchor domain (TIGR01167; HMM-score: 13.3)
  • TheSEED:  
    Citrate/H+ symporter of CitMHS family 
  • PFAM:
    IT (CL0182) CitMHS; Citrate transporter (PF03600; HMM-score: 131.5)
    DcuC; C4-dicarboxylate anaerobic carrier (PF03606; HMM-score: 26.9)
    DctM; Tripartite ATP-independent periplasmic transporter, DctM component (PF06808; HMM-score: 23.4)
    Na_H_antiporter; Na+/H+ antiporter family (PF03553; HMM-score: 21.6)
    Na_H_antiport_2; Na+-H+ antiporter family (PF13726; HMM-score: 18.3)
    no clan definedGram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 11.9)
    Caudo_bapla_RBP; Receptor-binding protein of phage tail base-plate Siphoviridae, head (PF08931; HMM-score: 11)
    LapA_dom; Lipopolysaccharide assembly protein A domain (PF06305; HMM-score: 9.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.163
    • Ymax_pos: 28
    • Cmax: 0.213
    • Cmax_pos: 28
    • Smax: 0.186
    • Smax_pos: 25
    • Smean: 0.117
    • D: 0.145
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMILGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVVIVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMVPWGGPMARVAAVLKAKSVNELWYGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAIERNELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKSWIKWVNTALTLAVILSMLINIAPPEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLKAIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSVSTAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAMLMGIVTI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]