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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2533 [new locus tag: NWMN_RS14555 ]
  • pan locus tag?: SAUPAN006363000
  • symbol: arcB
  • pan gene symbol?: arcB
  • synonym:
  • product: ornithine carbamoyltransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2533 [new locus tag: NWMN_RS14555 ]
  • symbol: arcB
  • product: ornithine carbamoyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 2786826..2787836
  • length: 1011
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGACAGAAATTCAAAAACCGTATGATTTAAAAGGCAGATCATTATTAAAAGAAAGTGAT
    TTTACCAAAGCAGAATTCGAAGGACTTATTGATTTTGCAATTACATTAAAAGAGTATAAG
    AAAAACGGTATTAAGCATCACTACTTATCTGGAAAAAATATTGCACTACTATTCGAAAAG
    AATTCGACGAGAACGCGTGCTGCGTTTACAGTTGCGTCTATTGATTTAGGTGCGCATCCA
    GAATTTTTAGGAAAAAATGATATTCAATTAGGCAAAAAAGAATCTGTAGAGGATACTGCG
    AAAGTATTAGGTAGAATGTTCGATGGTATTGAATTCCGTGGTTTTTCACAACAAGCTGTT
    GAAGATTTAGCGAAGTTCTCTGGTGTACCGGTGTGGAATGGATTAACAGACGATTGGCAT
    CCTACACAAATGTTAGCTGATTTTATGACAATAAAAGAGAATTTTGGATATCTAGAAGGA
    ATAAACTTAACTTACGTTGGAGATGGACGTAATAATATTGCGCATTCATTAATGGTAGCA
    GGTGCTATGTTAGGTGTTAATGTAAGAATTTGTACACCTAAATCATTAAATCCAAAAGAG
    GCATATGTTGATATTGCAAAAGAAAAAGCGAGTCAATATGGTGGTTCAGTCATGATTACG
    GATAATATTGCAGAAGCAGTTGAAAATACAGATGCTATATATACAGATGTTTGGGTATCG
    ATGGGTGAAGAAAGTGAATTTGAACAACGTATTAATTTATTAAAAGACTATCAAGTGAAT
    CAACAGATGTTTGATTTAACAGGTAAAGATTCAACGATATTCTTACATTGTTTACCAGCA
    TTCCATGATACAAATACACTTTATGGACAAGAAATTTATGAAAAATATGGATTAGCTGAA
    ATGGAAGTTACAGACCAAATCTTTAGAAGTGAACATTCAAAAGTGTTTGATCAAGCTGAA
    AATAGAATGCATACAATTAAGGCAGTAATGGCAGCAACATTGGGGAGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1011

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_2533 [new locus tag: NWMN_RS14555 ]
  • symbol: ArcB
  • description: ornithine carbamoyltransferase
  • length: 336
  • theoretical pI: 4.94437
  • theoretical MW: 37762.5
  • GRAVY: -0.331548

Function[edit source | edit]

  • reaction:
    EC 2.1.3.3?  ExPASy
    Ornithine carbamoyltransferaseCarbamoyl phosphate + L-ornithine = phosphate + L-citrulline
  • TIGRFAM:
    MetabolismAmino acid biosynthesisGlutamate familyornithine carbamoyltransferase (TIGR00658; EC 2.1.3.3; HMM-score: 414)
    MetabolismEnergy metabolismAmino acids and aminesputrescine carbamoyltransferase (TIGR04384; EC 2.1.3.6; HMM-score: 261.7)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPyrimidine ribonucleotide biosynthesisaspartate carbamoyltransferase (TIGR00670; EC 2.1.3.2; HMM-score: 125.5)
    knotted carbamoyltransferase YgeW (TIGR03316; EC 2.1.3.-; HMM-score: 70.9)
  • TheSEED:  
    Amino Acids and DerivativesArginine; urea cycle, polyaminesArginine and Ornithine Degradation Ornithine carbamoyltransferase (EC 2.1.3.3) 
    Arginine; urea cycle, polyaminesArginine Biosynthesis extended Ornithine carbamoyltransferase (EC 2.1.3.3) 
    Arginine; urea cycle, polyaminesArginine Deiminase Pathway Ornithine carbamoyltransferase (EC 2.1.3.3) 
  • PFAM:
    Asp-glut_race (CL0399) OTCace; Aspartate/ornithine carbamoyltransferase, Asp/Orn binding domain (PF00185; HMM-score: 196.1)
    no clan definedOTCace_N; Aspartate/ornithine carbamoyltransferase, carbamoyl-P binding domain (PF02729; HMM-score: 153.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    NWMN_0167(fadA)acetyl-CoA acetyltransferase-like protein  [1] (data from MRSA252)
    NWMN_0168(fadB)3-hydroxyacyl-CoA dehydrogenase FadB-like protein  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.126
    • Ymax_pos: 11
    • Cmax: 0.136
    • Cmax_pos: 68
    • Smax: 0.222
    • Smax_pos: 2
    • Smean: 0.147
    • D: 0.134
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGIKHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKVLGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEGINLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKEAYVDIAKEKASQYGGSVMITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQMFDLTGKDSTIFLHCLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATLGS

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulators: NWMN_1953 (Rex*), NWMN_2530 (ArcR*), AhrC (ArgR*), CcpA
    Rex* (TF) important in Energy metabolism  RegPrecise    transcription unit transferred from N315 data RegPrecise
    ArcR* (TF) important in Arginine degradation  RegPrecise    transcription unit transferred from N315 data RegPrecise
    AhrC  (ArgR*) (TF) important in Arginine biosynthesis, Arginine degradation  RegPrecise    transcription unit transferred from N315 data RegPrecise
    CcpA (TF) important in Carbon catabolism  RegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1

Relevant publications[edit source | edit]