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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2536 [new locus tag: NWMN_RS14575 ]
  • pan locus tag?: SAUPAN006367000
  • symbol: aur
  • pan gene symbol?: aur
  • synonym:
  • product: zinc metalloproteinase aureolysin

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2536 [new locus tag: NWMN_RS14575 ]
  • symbol: aur
  • product: zinc metalloproteinase aureolysin
  • replicon: chromosome
  • strand: -
  • coordinates: 2790267..2791796
  • length: 1530
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    GTGAGGAAATTTTCAAGATATGCATTTACAAGTATGGCAGCATTAACCTTGTTGAGCACT
    TTATCACCAGCAGCATTAGCGATTGATTCAAAAAATAAACCAGCTAATTCTGATATTAAA
    TTTGAGGTGACTCAAAAGAGTGATGCGGTCAAAGCATTAAAAGAATTGCCTAAATCCGAA
    AATGTAAAAAATATTTATCAAGATTACGCTGTTACTGATGTAAAAACTGATAAAAAAGGA
    TTTACGCATTATACATTGCAACCGAGTGTTGATGGTGTTCATGCACCTGACAAAGAAGTG
    AAAGTACACGCAGACAAATCAGGAAAAGTCGTTTTAATCAATGGGGATACTGATGCGAAG
    AAAGTAAAGCCAACGAATAAAGTGACATTAAGTAAAGATGACGCAGCCGACAAAGCATTT
    AAAGCAGTTAAGATTGATAAGAATAAAGCGAAAAATCTTAAAGATAAAGTCATTAAAGAA
    AACAAAGTTGAAATCGATGGTGACAGTAATAAATACGTTTATAATGTTGAGTTAATTACA
    GTGACACCAGAAATTTCACATTGGAAAGTTAAAATTGATGCTCAAACTGGCGAAATTTTA
    GAAAAAATGAACTTAGTTAAAGAAGCTGCAGAAACTGGTAAAGGAAAAGGTGTACTTGGC
    GATACAAAAGATATCAATATCAATAGTATTGACGGTGGATTTAGCCTAGAAGATTTAACG
    CATCAAGGTAAATTATCAGCATTTAGCTTTAATGATCAAACAGGTCAAGCAACATTGATT
    ACTAATGAAGATGAAAACTTCGTAAAAGATGAGCAACGTGCTGGCGTAGATGCAAATTAT
    TACGCTAAACAAACATATGATTATTACAAAGACACATTTGGTCGTGAATCATATGACAAC
    CAAGGTAGTCCAATTGTTTCATTAACGCATGTTAATAACTACGGTGGTCAAGATAACAGA
    AATAATGCCGCATGGATCGGTGACAAAATGATCTATGGTGATGGTGATGGTCGCACATTC
    ACAAGTTTATCGGGTGCAAATGACGTAGTAGCACACGAATTAACACACGGTGTGACACAA
    GAGACAGCGAACTTAGAATATAAGGACCAGTCAGGCGCTCTAAATGAAAGCTTTTCAGAT
    GTTTTTGGATACTTTGTAGATGACGAGGATTTCTTAATGGGTGAAGATGTCTACACACCT
    GGAAAAGAGGGAGACGCTTTACGCAGCATGTCAAACCCAGAACAATTTGGTCAACCAGCT
    CATATGAAAGACTATGTATTCACTGAAAAAGATAATGGTGGCGTACATACGAATTCTGGA
    ATTCCAAATAAAGCAGCTTATAACGTGATTCAAGCAATAGGGAAATCTAAATCAGAACAA
    ATTTACTACCGAGCATTAACGGAATACTTAACAAGTAATTCAAACTTCAAAGATTGTAAA
    GATGCATTATACCAAGCGGCTAAAGATTTATATGACGAGCAAACAGCTGAACAGGTGTAT
    GAAGCATGGAATGAAGTAGGCGTGGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1530

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_2536 [new locus tag: NWMN_RS14575 ]
  • symbol: Aur
  • description: zinc metalloproteinase aureolysin
  • length: 509
  • theoretical pI: 4.88318
  • theoretical MW: 56361
  • GRAVY: -0.700589

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    Listeria Pathogenicity Island LIPI-1 extended Zinc metalloproteinase precursor (EC 3.4.24.29) / aureolysin 
  • PFAM:
    Peptidase_MA (CL0126) Peptidase_M4_C; Thermolysin metallopeptidase, alpha-helical domain (PF02868; HMM-score: 176.1)
    Peptidase_M4; Thermolysin metallopeptidase, catalytic domain (PF01447; HMM-score: 142.8)
    PepSY (CL0320) PepSY; Peptidase propeptide and YPEB domain (PF03413; HMM-score: 50.1)
    Cystatin (CL0121) FTP; Fungalysin/Thermolysin Propeptide Motif (PF07504; HMM-score: 47.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: -0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: PAALAIDS
  • SignalP: Signal peptide length 27 aa
    • Ymax: 0.438
    • Ymax_pos: 27
    • Cmax: 0.206
    • Cmax_pos: 28
    • Smax: 0.964
    • Smax_pos: 11
    • Smean: 0.935
    • D: 0.632
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MRKFSRYAFTSMAALTLLSTLSPAALAIDSKNKPANSDIKFEVTQKSDAVKALKELPKSENVKNIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKVHADKSGKVVLINGDTDAKKVKPTNKVTLSKDDAADKAFKAVKIDKNKAKNLKDKVIKENKVEIDGDSNKYVYNVELITVTPEISHWKVKIDAQTGEILEKMNLVKEAAETGKGKGVLGDTKDININSIDGGFSLEDLTHQGKLSAFSFNDQTGQATLITNEDENFVKDEQRAGVDANYYAKQTYDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGDGDGRTFTSLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFLMGEDVYTPGKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVIQAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGVE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CodY
    CodY (TF) important in Amino acid metabolism  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]