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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2540 [new locus tag: NWMN_RS14595 ]
  • pan locus tag?: SAUPAN006372000
  • symbol: NWMN_2540
  • pan gene symbol?: manP
  • synonym:
  • product: PTS system, fructose-specific IIABC component

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2540 [new locus tag: NWMN_RS14595 ]
  • symbol: NWMN_2540
  • product: PTS system, fructose-specific IIABC component
  • replicon: chromosome
  • strand: +
  • coordinates: 2795654..2797606
  • length: 1953
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAAAATTGTAGCTATTACCTCATGCCCAAATGGCATTGCACATACATATATGGCTCAA
    GAAAAGCTTGAGCAGGTAGCAAAAGAAATGGGTGTGGATATTAAAGTAGAGACACAGGGC
    GGTGTTGGTGCTGAAAATGTTTTAACTACGCAAGACATCGAAGAAGCTGACGGTGTCATA
    ATTGCAGCTGATAAACAAGTTGATCTGTCCCGATTTGTTGGTAAACGGTTGATTAATGAA
    AATGTTCGCGAAGGGATTCATAATCCGAGAGGTCTAATTCAACGTATCATTAATCAAGAT
    GCGCCTATTTATCAATCTGAAACAAATTATCATTCGAAAGATCGCGGTAAGTCTAAAAGT
    GGTATTCAAATGGTGTATCAACATTTAATGAACGGTGTATCGTTTATGGTTCCTTTTATC
    GTAGTTGGTGGACTCCTTATCGCCATCGCGCTGACTCTAGGCGGTGAAACGACATCAAAA
    GGATTAGTCATCCCAGATGATTCATTTTGGAAATCCATTGAAAACATTGGTAGTTTAGCC
    TTTAAATTTATGGTTCCAATACTAGCTGGATATATCGCTGTTAGTATCGCTGACAAGCCC
    GGTCTTGTTCCTGGTATGATTGGCGGCGCTATCGCTGCTGACGGTAGTTTTTATGGCAGT
    GATGCAGGCGCAGGATTTTTAGGTGGTATTGTTGCTGGTTTCTTAGCAGGTTATATCGCA
    AAGTGGATTAAAGATATTAAAGTTCCAAAAGCAATGGCACCAATTATGCCTATCATTATT
    ATTCCCATTATTTCATCGGTCGTTGTAGGATTGATATTTATATTTTTAATCGGTGCACCA
    ATTTCTAATATTTTTGAGGCATTAACAACCTGGTTAAAAAGTATGCAAGGTGCTAATATT
    ATTATACTTGCGATGATTATAGGTGCGATGATTGCCTTCGATATGGGCGGTCCAGTAAAC
    AAGGTAGCATTTTTATTTGGCTCAGCACTTATTGCTGAAGGTAATTACGCCGTTATGGGT
    ATGGTCGCAGTTGCTGTATGTACACCACCAATCGGTTTAGGTTTAGCAACGTTTGTTCAA
    AAGTATAAATTCAATCATTCCGAAAGAGAAATGGGTAAGGCTTCCTTCACTATGGGACTA
    TTTGGTATTACTGAAGGTGCTATTCCTTTCGCAGCCCAAGATCCATTGCGCATTATACCT
    GCAAACATCATTGGTGCAATGATTGCATCAGTCATTGCAGCCATTGGTGGTGTCGGCGAT
    AGAGTCGCACATGGAGGTCCAATCGTGGCTGTATTAGGTGGTATTGATCATGTATTATGG
    TTTATCTTTGCAGTGATAGTAGGTAGTCTTGTCACAATGACTACTGTACTATTATTAAAA
    CGCAATACACCAGTTATAGCAGTAGATGCACCAGCGCAACACACGCAACTTCATGATACA
    GATATTACACAACATGATACAGAGGTTGACAATGTAGATAACACCGCGAAACATTCACAT
    CTCAATAAACCTTCTCATGTTTTTGATCAACAAACTATGATAATTACTGATCACGACATG
    TCTCGAAACGAAGCAATTGATATGTTGATTCATAAACTTAAAATTTGCCGTTATGTAGAG
    CACACATCGCATTTAAAAAATGCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATT
    GGCATGAACGTGGCCATTCCACATGCAAAGTCTGATGTTGTAAAACAACCGATTGTAGCT
    GTTATGAAAAATAATCATGGTGTTAAATGGGACAGTCTAGATGGTAGTCTACCTCAATTG
    ATATTCTTAATTGCCGTACCAAACAACAGTCAGGATACACATTTAAAAATACTTCAGCGC
    TTATCTAAAGCGTTAATGAATGATGAGACACGCCAATCACTAATTAATGCAAACAGTACT
    ACAGAAATTTATAACTTATTAATGAAAATATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1953

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_2540 [new locus tag: NWMN_RS14595 ]
  • symbol: NWMN_2540
  • description: PTS system, fructose-specific IIABC component
  • length: 650
  • theoretical pI: 6.77061
  • theoretical MW: 69855.3
  • GRAVY: 0.345077

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    PTS system, Fru family, IIC component (TIGR01427; HMM-score: 310.8)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, fructose subfamily, IIA component (TIGR00848; EC 2.7.1.69; HMM-score: 150.3)
    Signal transductionSignal transductionPTSPTS system, fructose subfamily, IIA component (TIGR00848; EC 2.7.1.69; HMM-score: 150.3)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, Fru family, IIB component (TIGR00829; EC 2.7.1.69; HMM-score: 112.1)
    Signal transductionSignal transductionPTSPTS system, Fru family, IIB component (TIGR00829; EC 2.7.1.69; HMM-score: 112.1)
    Signal transductionSignal transductionPTSPTS IIA-like nitrogen-regulatory protein PtsN (TIGR01419; HMM-score: 59.2)
    PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 19.2)
  • TheSEED:  
    Mannose Metabolism PTS system, mannose-specific IIB component (EC 2.7.1.69) / PTS system, mannose-specific IIC component / PTS system, mannose-specific IIA component 
    Sialic Acid Metabolism PTS system, mannose-specific IIB component (EC 2.7.1.69) / PTS system, mannose-specific IIC component / PTS system, mannose-specific IIA component 
  • PFAM:
    PTase-anion_tr (CL0340) PTS_EIIA_2; Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2 (PF00359; HMM-score: 115.3)
    PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 84.8)
    Phosphatase (CL0031) PTS_IIB; PTS system, Lactose/Cellobiose specific IIB subunit (PF02302; HMM-score: 60.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.25
    • Ymax_pos: 20
    • Cmax: 0.309
    • Cmax_pos: 20
    • Smax: 0.327
    • Smax_pos: 18
    • Smean: 0.189
    • D: 0.226
  • predicted transmembrane helices (TMHMM): 8

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKIVAITSCPNGIAHTYMAQEKLEQVAKEMGVDIKVETQGGVGAENVLTTQDIEEADGVIIAADKQVDLSRFVGKRLINENVREGIHNPRGLIQRIINQDAPIYQSETNYHSKDRGKSKSGIQMVYQHLMNGVSFMVPFIVVGGLLIAIALTLGGETTSKGLVIPDDSFWKSIENIGSLAFKFMVPILAGYIAVSIADKPGLVPGMIGGAIAADGSFYGSDAGAGFLGGIVAGFLAGYIAKWIKDIKVPKAMAPIMPIIIIPIISSVVVGLIFIFLIGAPISNIFEALTTWLKSMQGANIIILAMIIGAMIAFDMGGPVNKVAFLFGSALIAEGNYAVMGMVAVAVCTPPIGLGLATFVQKYKFNHSEREMGKASFTMGLFGITEGAIPFAAQDPLRIIPANIIGAMIASVIAAIGGVGDRVAHGGPIVAVLGGIDHVLWFIFAVIVGSLVTMTTVLLLKRNTPVIAVDAPAQHTQLHDTDITQHDTEVDNVDNTAKHSHLNKPSHVFDQQTMIITDHDMSRNEAIDMLIHKLKICRYVEHTSHLKNAILEREMESTTAIGMNVAIPHAKSDVVKQPIVAVMKNNHGVKWDSLDGSLPQLIFLIAVPNNSQDTHLKILQRLSKALMNDETRQSLINANSTTEIYNLLMKI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CcpA
    CcpA (TF) important in Carbon catabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]