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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2542 [new locus tag: NWMN_RS14605 ]
  • pan locus tag?: SAUPAN006374000
  • symbol: NWMN_2542
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2542 [new locus tag: NWMN_RS14605 ]
  • symbol: NWMN_2542
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2798670..2801657
  • length: 2988
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    GTGACAATGAAGAACGCATTTAAATTATTTAAAATGGATCTGAAGAAAGTAGCTAAGACG
    CCAGCTGTGTGGATTATCTTAGCAGGCTTAGCTATTTTGCCATCGTTCTACGCTTGGTTT
    AACTTATGGGCAATGTGGGATCCATATGGCAACACGGGACACATCAAGGTCGCAGTCGTT
    AATGAAGATAAAGGCGACACAATCAGAGGGAAAAAAGTTAATGTCGGTAATACGATGGTT
    AATACACTCAAGAAAAATAAAAGTTTTGATTGGCAGTTTGTAAGTAGAGAGAAAGCTGAT
    CATGAGATAAAAATGGGTAAATATTTTGCAGGTATTTACATCCCATCTAAGTTTACACAT
    GAAATTACAGGGACACTACGTAAGCAGCCTCAAAAAGCAGATGTAGAATTTAAGGTGAAT
    CAGAAGATTAACGCTGTTGCGTCTAAGCTAACAGATACTGGTTCGTCAGTTGTCGTTGAA
    AAAGCGAATGAACAATTTAATAAAACAGTAACTCGAGCATTATTAGAAGAAGCTAACAAA
    GCAGGTTTAACTATTGAAGAAAATGTGCCGACAATTAACAAGATAAAAAATGCGGTATAT
    TCAGCAGATAAAGCTTTACCTAAGATTAATGACTTTGCGAATAAAATTGTATATTTGAAT
    AACCACCAAGCGGATTTAGATAAATATGCCAATGATTTTAGAAAACTAGGAAATTATAAA
    GGTGATATTTTAGATGCTCAGAAAAAATTAAACGAAGTCAATGGTGCTATTCCGCAACTT
    AATGAAAAGGCTAAGTTGATATTAGCTTTAAATAATTATATGCCGAAAATTGAAAAAGCG
    TTAAATTTTGCAGCTGATGACGTGCCAGCGCAGTTCCCTAAAATTAATCAAGGACTTAAC
    ATTGCGAGTCAAGGTATTGATCAAGCTAATGGACAGTTAAATGATGCCAAAGGCTTCGTC
    ACACAAGTTAGAAGTAGAGTCGGTGATTATCAAGATGCAATTCGACGCGCGCAAGATTTA
    AATCGAAGAAACCAGCAACAGATTCCTCAAAATAGCGCGGCGAACAACGAAACATCAAAT
    AGTGCACCTGCAGCTGGTAATGGTGTAGCATCAACGCCACCAAGTGCACCAAGTGGCGAT
    ACTGCACCAAATAATAATGTTACGCAAAATACCGCACCAAATAGTAATAATGCGCCTGTA
    TCGACTACACCACAAAGTACAAGCGGGAAAAAAGATGGTCAAAGTTTTGTAGATATAACA
    ACAACACAAGTCAGCACAGCTAACGAGAACACACAAAACATTACAGATAAAGATGTTAAA
    TCAATGGAAGCGGCATTAACGGGCTCTTTATTATCATTATCAAATAATTTAGATACCCAA
    GCGAAAGCCGCACAAAAAGATAGTCAGGCATTACGTAATATTTCGTATGGGATTTTAGCA
    TCGGACAAGCCTTCTGATTTTAGAGAGTCTTTAGATAATGTTAAGTCCGGTTTAGAATAC
    ACAACGCAATATAATCAACAATTTATCGATACATTAAAAGAGATTGAGAAGAATGAAAAT
    GTTGATTTATCAAAAGAAATTGATAAGGTAAAAGCAGCTAATAATCGAATTAATGAATCA
    TTAAGGTTAGTTAATCAATTAAGCAATGCATTAAAGAATGGTAGTTCAGGAACTGCTGAA
    GCTACTAAATTACTAGATCAACTTTCAAAACTAGATTCATCATTATCATCATTTAGAGAT
    TATGTTAAAAAAGATCTTAACAGCTCTTTAGTATCAATATCACAACGTATTATGGATGAA
    TTGAACAAAGGGCAAACTGCATTATCCAATGTTCAGTCTAAATTAAATACAATTGATCAA
    GTCATTAACAGTGGACAAGCTATTTTAAAAAATGGTAAAACACGTATTGATCGCTTACAA
    ACAGTATTACCAAGTATTGAACAACAATACATTAGTGCTGTTAAAAATGCTCAAGCAAAC
    TTCTCGAAAGTGAAAAGTGATGTAGCTAAAGCTGCTAACTTTGTGCGCAATGACTTACCA
    CAGTTAGAACAGCGATTAACTAATGCGACAGCAAGTGTGAATAAAAATTTACCAACGTTA
    TTAAATGGTTATGATCAAGCGGTAGGATTACTAAATAAAAATCAGCCACAAGCGAAAAAG
    GCTTTATCAGATTTAGCTGATTTTTCTCAAAATAAATTGCCTGATGTTGAAAAAGATTTG
    AAAAAAGCGAATAAAATTTTCAAGAAATTAGACAAAGATGATGCAGTCGACAAATTAATC
    GACACACTTAAGAATGATTTGAAAAAGCAAGCGGGTATTATTGCAAATCCTATTAATAAG
    AAGACTGTTGATGTTTTCCCAGTTAAGGATTATGGTTCAGGTATGACACCATTCTATACT
    GCACTGTCAGTATGGGTAGGTGCACTCTTGATGGTAAGTTTATTAACGGTTGATAATAAA
    CATAAGAGTCTAGAGTCAGTCTTAACGACAAGACAAGTGTTCTTAGGTAAGGCAGGATTC
    TTTATAATGCTTGGTATGTTGCAAGCACTCATTGTATCGGTTGGAGATTTGTTAATCCTA
    AAAGCAGGAGTTGAGTCACCTGTATTATTTGTACTTATAACGATTTTCTGTTCGATTATT
    TTCAACTCAATCGTATATACGTGCGTATCATTACTTGGTAACCCAGGTAAAGCCATTGCA
    ATCGTATTGCTTGTATTACAAATTGCAGGTGGTGGGGGAACATTCCCAATTCAAACTACG
    CCACAATTTTTCCAAAACATTTCGCCATACTTACCATTTACGTATGCAATTGATTCATTA
    CGTGAAACAGTAGGCGGTATTGTTCCGGAAATCCTAATTACAAAATTAATTATATTAACG
    TTATTTGGTATAGGATTCTTCGTTGTAGGTTTAATTTTAAAACCTGTAACAGATCCATTG
    ATGAAGCGCGTATCTGAAAAAGTTGACCAAAGTAACGTTACAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    2988

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_2542 [new locus tag: NWMN_RS14605 ]
  • symbol: NWMN_2542
  • description: hypothetical protein
  • length: 995
  • theoretical pI: 9.90943
  • theoretical MW: 108948
  • GRAVY: -0.277487

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    YhgE/Pip C-terminal domain (TIGR03062; HMM-score: 167.6)
    YhgE/Pip N-terminal domain (TIGR03061; HMM-score: 158.5)
    type VII secretion protein EsaA (TIGR03929; HMM-score: 83.4)
    MetabolismTransport and binding proteinsOtherdaunorubicin resistance ABC transporter membrane protein (TIGR01247; HMM-score: 20.7)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtwin arginine-targeting protein translocase, TatA/E family (TIGR01411; HMM-score: 6.2)
  • TheSEED:  
    Phage infection protein 
  • PFAM:
    ABC-2 (CL0181) ABC2_membrane_3; ABC-2 family transporter protein (PF12698; HMM-score: 80.4)
    ABC2_membrane; ABC-2 type transporter (PF01061; HMM-score: 33.7)
    DUF3533; Protein of unknown function (DUF3533) (PF12051; HMM-score: 31.5)
    no clan definedADIP; Afadin- and alpha -actinin-Binding (PF11559; HMM-score: 18.8)
    6PGD_C (CL0106) 6PGD; 6-phosphogluconate dehydrogenase, C-terminal domain (PF00393; HMM-score: 16.4)
    no clan definedBaculo_PEP_C; Baculovirus polyhedron envelope protein, PEP, C terminus (PF04513; HMM-score: 15.2)
    NADP_Rossmann (CL0063) NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 11.2)
    Traffic (CL0147) V-SNARE_C; Snare region anchored in the vesicle membrane C-terminus (PF12352; HMM-score: 10.8)
    no clan definedDUF842; Eukaryotic protein of unknown function (DUF842) (PF05811; HMM-score: 10.7)
    DUF2935; Domain of unknown function (DUF2935) (PF11155; HMM-score: 8.2)
    Traffic (CL0147) Sec20; Sec20 (PF03908; HMM-score: 8.1)
    ABC-2 (CL0181) ABC2_membrane_5; ABC-2 family transporter protein (PF13346; HMM-score: 6.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.99
    • Cellwall Score: 0.01
    • Extracellular Score: 0.01
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.256
    • Ymax_pos: 14
    • Cmax: 0.152
    • Cmax_pos: 39
    • Smax: 0.769
    • Smax_pos: 7
    • Smean: 0.661
    • D: 0.414
  • predicted transmembrane helices (TMHMM): 6

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTMKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMWDPYGNTGHIKVAVVNEDKGDTIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIKMGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKANEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLNNHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIEKALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQDAIRRAQDLNRRNQQQIPQNSAANNETSNSAPAAGNGVASTPPSAPSGDTAPNNNVTQNTAPNSNNAPVSTTPQSTSGKKDGQSFVDITTTQVSTANENTQNITDKDVKSMEAALTGSLLSLSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFIDTLKEIEKNENVDLSKEIDKVKAANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQLSKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQTALSNVQSKLNTIDQVINSGQAILKNGKTRIDRLQTVLPSIEQQYISAVKNAQANFSKVKSDVAKAANFVRNDLPQLEQRLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFSQNKLPDVEKDLKKANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKDYGSGMTPFYTALSVWVGALLMVSLLTVDNKHKSLESVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLLILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFPIQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILKPVTDPLMKRVSEKVDQSNVTE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_2542 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]