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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2611 [new locus tag: NWMN_RS14950 ]
  • pan locus tag?: SAUPAN006488000
  • symbol: gidA
  • pan gene symbol?: gidA
  • synonym:
  • product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2611 [new locus tag: NWMN_RS14950 ]
  • symbol: gidA
  • product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA
  • replicon: chromosome
  • strand: -
  • coordinates: 2874610..2876487
  • length: 1878
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    GTGGTTCAAGAATATGATGTAATCGTTATAGGTGCGGGACATGCAGGTGTAGAAGCAGGT
    TTAGCATCTGCAAGACGTGGTGCTAAAACATTAATGCTAACAATAAATTTAGATAATATT
    GCATTTATGCCATGTAACCCATCTGTAGGTGGACCAGCTAAAGGTATCGTTGTTCGTGAA
    ATTGATGCTTTAGGTGGACAAATGGCAAAAACAATCGATAAAACACACATTCAAATGAGA
    ATGTTAAATACAGGTAAAGGACCTGCTGTAAGAGCACTAAGAGCGCAAGCAGATAAAGTA
    CTTTATCAACAAGAAATGAAACGCGTGATTGAAGATGAAGAAAATTTGCATATAATGCAA
    GGTATGGTAGACGAACTTATTATAGAAGATAATGAAGTTAAAGGTGTACGTACAAATATT
    GGTACAGAGTATTTATCTAAAGCAGTAATTATTACAACGGGAACATTTTTACGTGGTGAA
    ATCATTTTAGGTAATATGAAGTATTCAAGTGGACCAAATCACCAATTACCATCAATCACA
    TTATCAGACAATTTAAGAGAACTTGGTTTTGATATTGTTCGTTTTAAAACAGGTACACCA
    CCGCGTGTAAATTCAAAAACAATTGACTATTCGAAGACTGAAATACAACCAGGTGACGAT
    GTAGGTCGTGCATTCAGCTTTGAAACAACAGAATATATATTAGATCAATTGCCATGTTGG
    CTAACGTATACTAATGCTGAAACACACAAAGTTATCGATGATAATTTACATCTATCTGCA
    ATGTATTCAGGGATGATTAAAGGAACCGGGCCACGTTATTGCCCTTCAATTGAAGATAAA
    TTTGTTCGATTTAATGATAAGCCGCGACATCAACTTTTCTTAGAGCCTGAAGGTCGTAAT
    ACAAATGAAGTATATGTGCAAGGATTGTCTACAAGTCTTCCTGAACATGTGCAACGTCAA
    ATGTTAGAGACGATACCAGGTCTTGAAAAAGCAGATATGATGCGTGCCGGCTACGCAATT
    GAATATGATGCGATTGTGCCAACGCAGTTATGGCCTACACTTGAAACGAAAATGATTAAA
    AACTTATATACTGCAGGTCAAATTAATGGTACATCTGGTTATGAAGAAGCAGCAGGACAA
    GGATTGATGGCAGGTATTAACGCTGCAGGTAAAGTGTTAAACACAGGCGAAAAGATATTA
    AGTCGTTCAGATGCATATATTGGTGTCTTAATCGATGATCTTGTAACTAAAGGTACTAAT
    GAACCTTATCGTTTACTAACATCACGTGCAGAATATCGTTTGTTACTACGTCATGATAAT
    GCTGATTTGAGATTGACGGATATGGGATATGAACTTGGTATGATTTCTGAAGAAAGATAT
    GCACGTTTTAATGAAAAACGTCAGCAAATTGATGCGGAAATTAAGCGTTTATCAGATATT
    CGTATTAAACCAAACGAACATACGCAAGCGATTATTGAACAACATGGTGGTTCTCGCTTA
    AAAGATGGTATTTTAGCTATCGATTTATTACGCAGACCTGAAATGACTTACGATATAATT
    TTAGAACTTTTAGAAGAAGAACATCAATTGAATGCAGATGTTGAAGAACAAGTAGAAATA
    CAAACAAAATATGAAGGTTATATCAATAAATCACTACAACAAGTTGAGAAAGTTAAGCGT
    ATGGAAGAGAAGAAAATTCCAGAAGACTTAGATTATAGTAAGATTGATAGTTTGGCGACT
    GAAGCGCGAGAAAAATTATCAGAAGTAAAACCTTTAAATATTGCACAAGCTTCTAGAATA
    TCAGGGGTAAATCCAGCAGACATATCTATATTATTGATTTACTTAGAACAAGGTAAACTC
    CAAAGGGTGAGTGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1878

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_2611 [new locus tag: NWMN_RS14950 ]
  • symbol: GidA
  • description: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA
  • length: 625
  • theoretical pI: 5.14884
  • theoretical MW: 70115.6
  • GRAVY: -0.3928

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA (TIGR00136; HMM-score: 955.7)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA:m(5)U-54 methyltransferase (TIGR00137; EC 2.1.1.74; HMM-score: 59.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersChlorophyll and bacteriochlorphyllgeranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 23.6)
    MetabolismEnergy metabolismElectron transportthioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 23.3)
    Cellular processesCellular processesDetoxificationalkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 22.9)
    Cellular processesCellular processesAdaptations to atypical conditionsalkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 22.9)
    MetabolismEnergy metabolismAnaerobicglycerol-3-phosphate dehydrogenase, anaerobic, B subunit (TIGR03378; EC 1.1.5.3; HMM-score: 20.9)
    putative alkyl hydroperoxide reductase F subunit (TIGR03143; EC 1.6.4.-; HMM-score: 19.6)
    MetabolismEnergy metabolismAmino acids and aminessarcosine oxidase, monomeric form (TIGR01377; HMM-score: 16.2)
    MetabolismEnergy metabolismElectron transportglutathione-disulfide reductase (TIGR01424; EC 1.8.1.7; HMM-score: 15.4)
    MetabolismEnergy metabolismTCA cyclesuccinate dehydrogenase, flavoprotein subunit (TIGR01816; HMM-score: 15.3)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersChlorophyll and bacteriochlorphyllgeranylgeranyl reductase (TIGR02023; EC 1.3.1.-; HMM-score: 14.7)
    MetabolismEnergy metabolismAmino acids and aminessarcosine oxidase, alpha subunit family (TIGR01372; HMM-score: 13.6)
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 13.4)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiaminethiazole biosynthesis enzyme (TIGR00292; HMM-score: 12.1)
    MetabolismEnergy metabolismElectron transportflavocytochrome c (TIGR01813; HMM-score: 11.2)
    trypanothione-disulfide reductase (TIGR01423; EC 1.8.1.12; HMM-score: 10.7)
    Unknown functionEnzymes of unknown specificityputative bacillithiol system oxidoreductase, YpdA family (TIGR04018; EC 1.8.-.-; HMM-score: 10.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPyridine nucleotidesL-aspartate oxidase (TIGR00551; EC 1.4.3.16; HMM-score: 9.7)
    Cellular processesCellular processesDetoxificationmercury(II) reductase (TIGR02053; EC 1.16.1.1; HMM-score: 8.1)
    Unknown functionEnzymes of unknown specificityflavoprotein, HI0933 family (TIGR00275; HMM-score: 7.9)
  • TheSEED:  
    tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA 
  • PFAM:
    NADP_Rossmann (CL0063) GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 563.2)
    no clan definedGIDA_assoc; GidA associated domain (PF13932; HMM-score: 267.7)
    NADP_Rossmann (CL0063) FAD_binding_2; FAD binding domain (PF00890; HMM-score: 22.7)
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 22.4)
    FAD_oxidored; FAD dependent oxidoreductase (PF12831; HMM-score: 20.2)
    Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 15.1)
    Thi4; Thi4 family (PF01946; HMM-score: 11.8)
    HI0933_like; HI0933-like protein (PF03486; HMM-score: 10.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: FAD
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.136
    • Ymax_pos: 44
    • Cmax: 0.173
    • Cmax_pos: 25
    • Smax: 0.279
    • Smax_pos: 39
    • Smean: 0.11
    • D: 0.126
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMPCNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLYQQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGEIILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPGDDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPSIEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMMRAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKVLNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGYELGMISEERYARFNEKRQQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAIDLLRRPEMTYDIILELLEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKIPEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVSD

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]