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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS02905 [old locus tag: NWMN_0498 ]
  • pan locus tag?: SAUPAN002305000
  • symbol: NWMN_RS02905
  • pan gene symbol?: nusG
  • synonym:
  • product: transcription termination/antitermination protein NusG

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS02905 [old locus tag: NWMN_0498 ]
  • symbol: NWMN_RS02905
  • product: transcription termination/antitermination protein NusG
  • replicon: chromosome
  • strand: +
  • coordinates: 571845..572393
  • length: 549
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_009641 (571845..572393) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_0498

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAA
    AATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAATC
    TTTAGAGTAGTCATACCGGAAGAAGAAGAAACTCAAGTAAAAGATGGCAAAGCTAAAACG
    ACTGTTAAAAAAACATTCCCTGGATATGTTTTAGTGGAATTAATCATGACAGATGAATCA
    TGGTATGTGGTAAGAAATACACCAGGCGTTACTGGTTTTGTAGGTTCTGCAGGTGCAGGG
    TCTAAGCCAAATCCATTGTTACCAGAAGAAGTTCGCTTCATCTTAAAACAAATGGGTCTT
    AAAGAAAAGACTATCGATGTTGAACTCGAAGTTGGCGAGCAAGTTCGTATTAAATCAGGT
    CCATTTGCGAATCAAGTTGGTGAAGTTCAAGAAATTGAAACAGATAAGTTTAAGCTAACA
    GTATTAGTAGATATGTTTGGCCGAGAAACACCAGTAGAAGTTGAATTCGATCAAATAGAA
    AAGCTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    549

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_RS02905 [old locus tag: NWMN_0498 ]
  • symbol: NWMN_RS02905
  • description: transcription termination/antitermination protein NusG
  • length: 182
  • theoretical pI: 4.78638
  • theoretical MW: 20663.5
  • GRAVY: -0.423626

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Transcription Transcription factors transcription termination/antitermination factor NusG (TIGR00922; HMM-score: 203.6)
    and 3 more
    NusG family protein (TIGR01956; HMM-score: 106.4)
    Genetic information processing Transcription Transcription factors transcription elongation factor/antiterminator RfaH (TIGR01955; HMM-score: 61.1)
    transcription elongation factor Spt5 (TIGR00405; HMM-score: 27)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    NusG-like (CL0439) NusG; Transcription termination factor nusG (PF02357; HMM-score: 96.1)
    and 1 more
    KOW (CL0107) KOW; KOW motif (PF00467; HMM-score: 29)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005909
    • TAT(Tat/SPI): 0.000544
    • LIPO(Sec/SPII): 0.00119
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVVIPEEEETQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSKPNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLTVLVDMFGRETPVEVEFDQIEKL

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]