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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS02940 [old locus tag: NWMN_0505 ]
  • pan locus tag?: SAUPAN002315000
  • symbol: NWMN_RS02940
  • pan gene symbol?: rpoC
  • synonym:
  • product: DNA-directed RNA polymerase subunit beta'

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS02940 [old locus tag: NWMN_0505 ]
  • symbol: NWMN_RS02940
  • product: DNA-directed RNA polymerase subunit beta'
  • replicon: chromosome
  • strand: +
  • coordinates: 579792..583388
  • length: 3597
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (579792..583388) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

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    ATGAAAATAGGATTGGCTTCACCTGAAAAAATCCGTTCTTGGTCTTTTGGTGAAGTTAAA
    AAACCTGAAACAATCAACTACCGTACATTAAAACCTGAAAAAGATGGTCTATTCTGTGAA
    AGAATTTTCGGACCTACAAAAGACTGGGAATGTAGTTGTGGTAAATACAAACGTGTTCGC
    TACAAAGGCATGGTCTGTGACAGATGTGGAGTTGAAGTAACTAAATCTAAAGTACGTCGT
    GAAAGAATGGGTCACATTGAACTTGCTGCTCCAGTTTCTCACATTTGGTATTTCAAAGGT
    ATACCAAGTCGTATGGGATTATTACTTGACATGTCACCAAGAGCATTAGAAGAAGTTATT
    TACTTTGCTTCTTATGTTGTTGTAGATCCAGGTCCAACTGGTTTAGAAAAGAAAACTTTA
    TTATCTGAAGCTGAATTCAGAGATTATTATGATAAATACCCAGGTCAATTCGTTGCAAAA
    ATGGGTGCAGAAGGTATTAAAGATTTACTTGAAGAGATTGATCTTGACGAAGAACTTAAA
    TTGTTACGCGATGAGTTGGAATCAGCTACTGGTCAAAGACTTACTCGTGCAATTAAACGT
    TTAGAAGTTGTTGAATCATTCCGTAATTCAGGTAACAAACCTTCATGGATGATTTTAGAT
    GTACTTCCAATCATCCCACCAGAAATTCGTCCAATGGTTCAATTAGATGGTGGACGATTT
    GCAACAAGTGACTTAAACGACTTATACCGTCGTGTAATTAATCGAAATAATCGTTTGAAA
    CGTTTATTAGATTTAGGTGCACCTGGTATCATCGTTCAAAACGAAAAACGTATGTTACAA
    GAAGCCGTTGACGCTTTAATTGATAATGGTCGTCGTGGTCGTCCAGTTACTGGCCCAGGT
    AACCGTCCATTAAAATCTTTATCTCATATGTTAAAAGGTAAACAAGGTCGTTTCCGTCAA
    AACTTACTTGGTAAACGTGTTGACTATTCAGGACGTTCAGTTATTGCAGTAGGTCCAAGC
    TTGAAAATGTACCAATGTGGTTTACCAAAAGAAATGGCACTTGAACTATTTAAACCATTC
    GTAATGAAAGAATTAGTTCAACGTGAAATTGCAACTAACATTAAAAATGCGAAGAGTAAA
    ATCGAACGTATGGATGATGAAGTTTGGGACGTATTGGAAGAAGTAATTAGAGAACATCCT
    GTATTACTTAACCGTGCACCAACACTTCATAGACTTGGTATTCAAGCATTTGAACCAACT
    TTAGTTGAAGGTCGTGCGATTCGTCTACATCCACTTGTAACAACAGCTTATAACGCTGAC
    TTTGACGGTGACCAAATGGCGGTTCACGTTCCTTTATCAAAAGAGGCACAAGCTGAAGCA
    AGAATGTTGATGTTAGCAGCACAAAACATCTTGAACCCTAAAGATGGTAAACCTGTAGTT
    ACACCATCACAAGATATGGTACTTGGTAACTATTACCTTACTTTAGAAAGAAAAGATGCA
    GTAAATACAGGCGCAATCTTTAATAATACAAATGAAGTATTAAAAGCATATGCAAATGGC
    TTTGTACATTTACACACTAGAATTGGTGTACATGCAAGTTCGTTCAATAATCCAACATTT
    ACTGAAGAACAAAACAAAAAGATTCTTGCTACGTCAGTAGGTAAAATTATATTCAATGAA
    ATCATTCCAGATTCATTTGCTTATATTAATGAACCTACGCAAGAAAACTTAGAAAGAAAG
    ACACCAAACAGATATTTCATCGATCCTACAACTTTAGGTGAAGGTGGATTAAAAGAATAC
    TTTGAAAATGAAGAATTAATTGAACCTTTCAACAAAAAATTCTTAGGTAATATTATTGCA
    GAAGTATTCAACAGATTTAGCATCACTGATACATCAATGATGTTAGACCGTATGAAAGAC
    TTAGGATTCAAATTCTCATCTAAAGCTGGTATTACAGTAGGTGTTGCTGATATCGTAGTA
    TTACCTGATAAGCAACAAATACTTGATGAGCATGAAAAATTAGTCGACAGAATTACAAAA
    CAATTCAACCGTGGTTTAATCACTGAAGAAGAAAGATATAATGCAGTTGTTGAAATTTGG
    ACAGATGCAAAAGATCAAATTCAAGGTGAATTGATGCAATCACTTGATAAAACTAACCCA
    ATCTTCATGATGAGTGATTCAGGTGCCCGTGGTAACGCATCTAACTTTACACAGTTAGCA
    GGTATGCGTGGATTGATGGCCGCACCATCTGGTAAGATTATCGAATTACCAATCACATCT
    TCATTCCGTGAAGGTTTAACAGTACTTGAATACTTCATCTCAACTCACGGTGCACGTAAA
    GGTCTTGCCGATACAGCACTTAAAACAGCTGACTCAGGATATCTTACTCGTCGTCTTGTT
    GACGTGGCACAAGATGTTATTGTTCGTGAAGAAGACTGTGGTACTGATAGAGGTTTATTA
    GTTTCTGATATTAAAGAAGGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGT
    CGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGAT
    GAATTAATTACACCTGAAATTGCTAAGAAAATTACAGATGCTGGTATTGAACAAATGTAT
    ATTCGCTCAGCATTTACTTGTAACGCACGACATGGTGTTTGTGAAAAATGTTACGGTAAA
    AACCTTGCTACTGGTGAAAAAGTTGAAGTTGGTGAAGCAGTTGGTACAATTGCAGCCCAA
    TCTATCGGTGAACCAGGTACACAGCTTACAATGCGTACATTCCATACAGGTGGGGTAGCA
    GGTAGCGATATCACACAAGGTCTTCCTCGTATTCAAGAGATTTTCGAAGCACGTAACCCT
    AAAGGTCAAGCGGTAATTACGGAAATCGAAGGTGTCGTAGAAGATATTAAATTAGCAAAA
    GATAGACAACAAGAAATTGTTGTTAAAGGTGCTAATGAAACAAGATCATACCTTGCTTCA
    GGTACTTCAAGAATTATTGTAGAAATCGGTCAACCAGTTCAACGTGGTGAAGTATTAACT
    GAAGGTTCTATTGAACCTAAGAATTACTTATCTGTTGCTGGATTAAACGCGACTGAAAGC
    TACTTATTAAAAGAAGTACAAAAAGTTTACCGTATGCAAGGTGTAGAAATCGACGATAAA
    CACGTTGAGGTTATGGTTCGACAAATGTTACGTAAAGTTAGAATTATCGAAGCAGGTGAT
    ACGAAGTTATTACCAGGTTCATTAGTTGATATTCATAACTTTACAGATGCAAATAGAGAA
    GCATTTAAACACCGTAAGCGTCCTGCAACAGCTAAACCAGTATTACTTGGTATTACTAAA
    GCATCACTTGAAACAGAAAGTTTCTTATCTGCAGCATCATTCCAAGAAACAACAAGAGTT
    CTTACAGATGCAGCAATTAAAGGTAAGCGTGATGACTTATTAGGTCTTAAAGAAAACGTA
    ATTATTGGTAAGTTAATTCCAGCTGGTACTGGTATGAGACGTTATAGCGACGTAAAATAC
    GAAAAAACAGCTAAACCAGTTGCAGAAGTTGAATCTCAAACTGAAGTAACGGAATAA
    60
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    3597

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS02940 [old locus tag: NWMN_0505 ]
  • symbol: NWMN_RS02940
  • description: DNA-directed RNA polymerase subunit beta'
  • length: 1198
  • theoretical pI: 7.01614
  • theoretical MW: 134273
  • GRAVY: -0.354341

Function[edit source | edit]

  • reaction:
    EC 2.7.7.6?  ExPASy
    DNA-directed RNA polymeraseNucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
  • TIGRFAM:
    Genetic information processingTranscriptionDNA-dependent RNA polymeraseDNA-directed RNA polymerase, beta' subunit (TIGR02386; EC 2.7.7.6; HMM-score: 1883.6)
    DNA-directed RNA polymerase, gamma subunit (TIGR02387; EC 2.7.7.6; HMM-score: 843.5)
    Genetic information processingTranscriptionDNA-dependent RNA polymeraseDNA-directed RNA polymerase, beta subunit (TIGR02388; EC 2.7.7.6; HMM-score: 645.3)
    DNA-directed RNA polymerase subunit A' (TIGR02390; EC 2.7.7.6; HMM-score: 320.8)
    Genetic information processingTranscriptionDNA-dependent RNA polymeraseDNA-directed RNA polymerase, subunit A (TIGR02389; EC 2.7.7.6; HMM-score: 115)
  • TheSEED:
  • PFAM:
    no clan definedRNA_pol_Rpb1_1; RNA polymerase Rpb1, domain 1 (PF04997; HMM-score: 294.8)
    RNA_pol_Rpb1_5; RNA polymerase Rpb1, domain 5 (PF04998; HMM-score: 233.5)
    RNA_pol_Rpb1_2; RNA polymerase Rpb1, domain 2 (PF00623; HMM-score: 140.1)
    RNA_pol_Rpb1_3; RNA polymerase Rpb1, domain 3 (PF04983; HMM-score: 117.5)
    RNA_pol_Rpb1_4; RNA polymerase Rpb1, domain 4 (PF05000; HMM-score: 54.7)
    Hybrid (CL0105) Peptidase_M23; Peptidase family M23 (PF01551; HMM-score: 17.6)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.119
    • Ymax_pos: 12
    • Cmax: 0.123
    • Cmax_pos: 23
    • Smax: 0.178
    • Smax_pos: 1
    • Smean: 0.134
    • D: 0.125
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI:
  • RefSeq: WP_001788197 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFGPTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIPSRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAKMGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMILDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSVIAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVLEEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHVPLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFNNTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSFAYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFNRFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQFNRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLAGMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRRLVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIRHPETDEIIIRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGVVEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYLSVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSLVDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAIKGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS02940 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]