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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS03275 [old locus tag: NWMN_0572 ]
  • pan locus tag?: SAUPAN002463000
  • symbol: NWMN_RS03275
  • pan gene symbol?: iolS
  • synonym:
  • product: aldo/keto reductase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS03275 [old locus tag: NWMN_0572 ]
  • symbol: NWMN_RS03275
  • product: aldo/keto reductase
  • replicon: chromosome
  • strand: +
  • coordinates: 653120..654058
  • length: 939
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (653120..654058) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAGCAATTTGTAAACTTAGGTAAATCTGATGTTGAAGTGTTTCCAATCGCACTTGGG
    ACGAACGCAGTAGGTGGGCATAATTTATATCCGAACTTAGATGAAGAACAAGGAAAAGAT
    GTTGTTCGTCAAGCCATTAATCATGGTATTAATTTATTAGATACGGCATATATTTATGGG
    CCAGAACGATCAGAAGAATTGGTTGGAGAAGTTGTTAAAGAATATCCGCGAGAGCAAATT
    AAAATTGCTACGAAAGGGTCTCATGAATTTGATGAAAATCAAGAAGTACATCAGAACAAT
    CAACCGGAATATTTAAAACAACAAGTTGAGAATAGTTTGAAACGTCTACAAACTGATTAT
    ATCGATTTATATTATATTCATTTTCCGGATAACAACACTCCGAAAGATCAAGCAGTTGCA
    GCATTACAAGAGCTTAAGGAACAAGGGAAGATTAAAGCAATTGGTGTATCAAATTTCACA
    TTAGATCAACTTAAAGAAGCAAATAAAGATGGTTACGTTGATGTTGTACAGTTAGAATAT
    AATTTATTGCATCGCGAAAATGAGGCAGTATTACAATATTGTGTTGATCACCAAATCACA
    TTTATTCCATATTTCCCATTAGCATCCGGTATTTTAGCTGGAAAATATGATGAGAACACT
    AAATTTAGTGACCATCGTACTACACGTCGTGATTTTAAACCAGGTGTATTTGAAGAAAAT
    GTGCGTCGCGTAAAAGCTTTGGAAAGCATAGCTGCAGCACATCAAACTTCAATTGCGAAC
    ATTGTATTAGCATTTTATTTAACGAGACCAGCTATCGATGTGATTATTCCTGGTGCAAAA
    CGTGCAGAACAAGTCGTTGAAAATATTAAAGCTGCAGATATCGTTTTATCAGATGATGAG
    ATTCAATATATCGATGAACTGTTTCCGATTGAAGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    939

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS03275 [old locus tag: NWMN_0572 ]
  • symbol: NWMN_RS03275
  • description: aldo/keto reductase
  • length: 312
  • theoretical pI: 4.6314
  • theoretical MW: 35459.5
  • GRAVY: -0.465385

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    voltage-dependent potassium channel beta subunit (TIGR01293; HMM-score: 90.4)
  • TheSEED:
  • PFAM:
    no clan definedAldo_ket_red; Aldo/keto reductase family (PF00248; HMM-score: 255.8)
    NADP_Rossmann (CL0063) Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 16.3)
    HAD (CL0137) Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 13.7)
    no clan definedRasGAP_C; RasGAP C-terminus (PF03836; HMM-score: 13)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.152
    • Ymax_pos: 27
    • Cmax: 0.11
    • Cmax_pos: 27
    • Smax: 0.263
    • Smax_pos: 22
    • Smean: 0.194
    • D: 0.169
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446731646 NCBI
  • RefSeq: WP_000808902 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQAINHGINLLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQPEYLKQQVENSLKRLQTDYIDLYYIHFPDNNTPKDQAVAALQELKEQGKIKAIGVSNFTLDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHQITFIPYFPLASGILAGKYDENTKFSDHRTTRRDFKPGVFEENVRRVKALESIAAAHQTSIANIVLAFYLTRPAIDVIIPGAKRAEQVVENIKAADIVLSDDEIQYIDELFPIED

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS03275 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]