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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS05890 [old locus tag: NWMN_1041 ]
  • pan locus tag?: SAUPAN003359000
  • symbol: NWMN_RS05890
  • pan gene symbol?: isdA
  • synonym:
  • product: iron-regulated surface determinant protein A

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS05890 [old locus tag: NWMN_1041 ]
  • symbol: NWMN_RS05890
  • product: iron-regulated surface determinant protein A
  • replicon: chromosome
  • strand: -
  • coordinates: 1144220..1145272
  • length: 1053
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (1144220..1145272) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGACAAAACATTATTTAAACAGTAAGTATCAATCAGAACAACGTTCATCAGCTATGAAA
    AAGATTACAATGGGTACAGCATCTATCATTTTAGGTTCCCTTGTATACATAGGCGCAGAC
    AGCCAACAAGTCAATGCGGCAACAGAAGCTACGAACGCAACTAATAATCAAAGCACACAA
    GTTTCTCAAGCAACATCACAACCAATTAATTTCCAAGTGCAAAAAGATGGCTCTTCAGAG
    AAGTCACACATGGATGACTATATGCAACACCCTGGTAAAGTAATTAAACAAAATAATAAA
    TATTATTTCCAAACCGTGTTAAACAATGCATCATTCTGGAAAGAATACAAATTTTACAAT
    GCAAACAATCAAGAATTAGCAACAACTGTTGTTAACGATAATAAAAAAGCGGATACTAGA
    ACAATCAATGTTGCAGTTGAACCTGGATATAAGAGCTTAACTACTAAAGTACATATTGTC
    GTGCCACAAATTAATTACAATCATAGATATACTACGCATTTGGAATTTGAAAAAGCAATT
    CCTACATTAGCTGACGCAGCAAAACCAAACAATGTTAAACCGGTTCAACCAAAACCAGCT
    CAACCTAAAACACCTACTGAGCAAACTAAACCAGTTCAACCTAAAGTTGAAAAAGTTAAA
    CCTACTGTAACTACAACAAGCAAAGTTGAAGACAATCACTCTACTAAAGTTGTAAGTACT
    GACACAACAAAAGATCAAACTAAAACACAAACTGCTCATACAGTTAAAACAGCACAAACT
    GCTCAAGAACAAAATAAAGTTCAAACACCTGTTAAAGATGTTGCAACAGCGAAATCTGAA
    AGCAACAATCAAGCTGTAAGTGATAATAAATCACAACAAACTAACAAAGTTACAAAACAT
    AACGAAACGCCTAAACAAGCATCTAAAGCTAAAGAATTACCAAAAACTGGTTTAACTTCA
    GTTGATAACTTTATTAGCACAGTTGCCTTCGCAACACTTGCCCTTTTAGGTTCATTATCT
    TTATTACTTTTCAAAAGAAAAGAATCTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1053

Protein[edit source | edit]

Protein Data Bank: 3QZL
Protein Data Bank: 3QZM
Protein Data Bank: 3QZN
Protein Data Bank: 3QZO
Protein Data Bank: 3QZP
Protein Data Bank: 2ITE
Protein Data Bank: 2ITF

General[edit source | edit]

  • locus tag: NWMN_RS05890 [old locus tag: NWMN_1041 ]
  • symbol: NWMN_RS05890
  • description: iron-regulated surface determinant protein A
  • length: 350
  • theoretical pI: 10.2642
  • theoretical MW: 38745.2
  • GRAVY: -0.817714

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    heme uptake protein IsdC (TIGR03656; HMM-score: 60.1)
    Cell structureCell envelopeOtherLPXTG cell wall anchor domain (TIGR01167; HMM-score: 22.5)
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 16)
    pullulanase, extracellular (TIGR02102; HMM-score: 9.8)
  • TheSEED:
  • PFAM:
    no clan definedNEAT; Iron Transport-associated domain (PF05031; HMM-score: 87.5)
    Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 37.1)
    DUF5044; Domain of unknown function (DUF5044) (PF16447; HMM-score: 15.3)
    YjbE; Exopolysaccharide production protein YjbE (PF11106; HMM-score: 13)
    post-AAA (CL0604) MgsA_C; MgsA AAA+ ATPase C terminal (PF12002; HMM-score: 6.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 2
  • LocateP:
  • SignalP: Signal peptide length 47 aa
    • Ymax: 0.618
    • Ymax_pos: 47
    • Cmax: 0.504
    • Cmax_pos: 47
    • Smax: 0.843
    • Smax_pos: 37
    • Smean: 0.598
    • D: 0.61
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

  • GI: 446083004 NCBI
  • RefSeq: WP_000160859 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQVNAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYYFQTVLNNASFWKEYKFYNANNQELATTVVNDNKKADTRTINVAVEPGYKSLTTKVHIVVPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEKVKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVATAKSESNNQAVSDNKSQQTNKVTKHNETPKQASKAKELPKTGLTSVDNFISTVAFATLALLGSLSLLLFKRKESK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS05890 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]