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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS06245 [old locus tag: NWMN_1103 ]
  • pan locus tag?: SAUPAN003465000
  • symbol: NWMN_RS06245
  • pan gene symbol?: ileS
  • synonym:
  • product: isoleucine--tRNA ligase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS06245 [old locus tag: NWMN_1103 ]
  • symbol: NWMN_RS06245
  • product: isoleucine--tRNA ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 1207087..1209840
  • length: 2754
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (1207087..1209840) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGATTACAAAGAAACGTTATTAATGCCTAAAACAGATTTCCCAATGCGAGGTGGTTTA
    CCAAACAAGGAACCGCAAATTCAAGAAAAATGGGATGCAGAAGATCAATACCATAAAGCG
    TTAGAAAAAAATAAAGGTAACGAAACATTCATTTTACATGATGGCCCACCATACGCGAAT
    GGTAACTTACATATGGGACATGCCTTGAACAAAATTTTAAAAGACTTTATTGTACGTTAT
    AAAACTATGCAAGGGTTCTATGCACCATACGTACCAGGTTGGGATACACATGGTTTACCA
    ATTGAACAAGCATTAACGAAAAAAGGTGTTGACCGAAAGAAAATGTCAACAGCTGAATTC
    CGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGA
    CGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAA
    GCTGCACAAATTCGTATTTTTGGAGAAATGGCAGATAAAGGTTTAATTTATAAAGGTAAA
    AAGCCAGTTTATTGGTCTCCTTCAAGTGAGTCTTCATTAGCAGAAGCAGAAATTGAATAT
    CACGATAAACGTTCAGCATCAATTTACGTTGCATTTGACGTTAAAGATGACAAAGGTGTC
    GTTGATGCAGATGCTAAATTTATTATCTGGACAACAACGCCATGGACAATTCCATCAAAT
    GTTGCGATTACCGTTCATCCTGAATTAAAATATGGTCAATACAATGTAAATGGCGAAAAA
    TATATTATTGCAGAAGCCTTGTCTGACGCTGTAGCAGAAGCACTGGATTGGGATAAAGCA
    TCAATCAAATTAGAAAAAGAATACACAGGTAAAGAATTAGAGTATGTTGTAGCACAACAT
    CCATTCTTAGACAGAGAATCGTTAGTGATTAATGGTGATCATGTTACTACAGATGCTGGT
    ACAGGTTGTGTACATACAGCACCAGGTCACGGGGAAGATGACTATATTGTTGGTCAAAAA
    TATGAATTGCCAGTAATTAGTCCAATCGATGATAAAGGTGTATTTACTGAAGAAGGCGGC
    CAATTTGAAGGGATGTTCTATGATAAAGCTAATAAAGCCGTTACTGATTTATTAACAGAA
    AAAGGTGCACTATTAAAATTAGACTTTATTACACATAGCTATCCACACGACTGGAGAACA
    AAAAAACCTGTAATCTTCCGTGCTACACCACAATGGTTTGCCTCAATCAGTAAAGTAAGA
    CAAGATATTTTAGATGCAATCGAAAATACAAACTTCAAAGTAAATTGGGGTAAAACACGT
    ATTTACAATATGGTTCGTGACCGTGGCGAATGGGTTATTTCTCGTCAACGTGTGTGGGGT
    GTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTG
    AATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCG
    AAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTACATTTACTAAA
    GAAACAGACATTATGGACGTTTGGTTTGATTCTGGTTCATCACACCGTGGCGTGTTGGAA
    ACAAGACCGGAATTAAGTTTCCCAGCGGATATGTATTTAGAAGGTAGTGACCAATATCGT
    GGTTGGTTCAACTCTTCTATCACAACTTCAGTTGCTACAAGAGGAGTATCACCTTATAAA
    TTCTTACTTTCTCATGGTTTTGTTATGGACGGTGAAGGTAAGAAAATGAGTAAATCTTTA
    GGTAATGTGATTGTACCTGACCAAGTGGTTAAACAAAAAGGTGCTGATATTGCGAGACTT
    TGGGTAAGTAGTACGGACTATTTAGCTGATGTTAGAATTTCTGATGAAATTTTAAAACAA
    ACATCTGATGTTTATCGTAAAATCAGAAATACATTAAGATTTATGTTAGGTAACATTAAC
    GATTTCAATCCTGACACAGATAGCATTCCTGAATCAGAGTTATTAGAAGTGGATCGTTAC
    TTGCTAAATCGTTTACGTGAATTTACTGCAAGTACGATTAACAACTATGAAAACTTTGAC
    TACTTAAATATTTATCAAGAAGTTCAAAACTTTATCAATGTTGAGTTAAGTAATTTCTAT
    TTGGATTACGGTAAAGATATTTTATATATTGAACAACGTGATTCTCATATCCGTCGTAGT
    ATGCAAACAGTGTTATATCAAATTTTAGTTGATATGACGAAGTTGTTAGCACCAATCTTA
    GTGCATACAGCTGAAGAAGTTTGGTCTCATACACCACATGTTAAAGAAGAAAGTGTTCAC
    TTAGCAGACATGCCTAAAGTTGTAGAAGTAGATCAAGCTTTATTGGATAAATGGCGTACA
    TTTATGAATTTACGTGATGATGTGAACCGTGCATTAGAAACTGCTCGTAATGAAAAAGTT
    ATTGGTAAATCATTAGAAGCTAAAGTTACGATTGCTAGTAACGATAAATTTAATGCATCT
    GAATTCTTAACTTCATTTGATGCATTACATCAATTATTTATCGTGTCACAAGTTAAAGTT
    GTAGATAAGTTAGACGATCAGGCAACAGCTTATGAACATGGTGATATTGTCATCGAACAT
    GCAGATGGTGAAAAATGTGAAAGATGTTGGAACTATTCAGAGGATCTTGGTGCTGTTGAT
    GAATTGACGCATCTATGTCCACGATGCCAACAAGTTGTAAAATCACTTGTATAA
    60
    120
    180
    240
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    480
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    720
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    840
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    1020
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    1140
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    2160
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    2400
    2460
    2520
    2580
    2640
    2700
    2754

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS06245 [old locus tag: NWMN_1103 ]
  • symbol: NWMN_RS06245
  • description: isoleucine--tRNA ligase
  • length: 917
  • theoretical pI: 5.13287
  • theoretical MW: 104885
  • GRAVY: -0.467612

Function[edit source | edit]

  • reaction:
    EC 6.1.1.5?  ExPASy
    Isoleucine--tRNA ligaseATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile)
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA aminoacylationisoleucine--tRNA ligase (TIGR00392; EC 6.1.1.5; HMM-score: 990.2)
    Genetic information processingProtein synthesistRNA aminoacylationvaline--tRNA ligase (TIGR00422; EC 6.1.1.9; HMM-score: 471.3)
    Genetic information processingProtein synthesistRNA aminoacylationleucine--tRNA ligase (TIGR00396; EC 6.1.1.4; HMM-score: 157.1)
    Genetic information processingProtein synthesistRNA aminoacylationleucine--tRNA ligase (TIGR00395; EC 6.1.1.4; HMM-score: 156)
    Genetic information processingProtein synthesistRNA aminoacylationmethionine--tRNA ligase (TIGR00398; EC 6.1.1.10; HMM-score: 120.9)
    Genetic information processingProtein synthesistRNA aminoacylationcysteine--tRNA ligase (TIGR00435; EC 6.1.1.16; HMM-score: 42.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersGlutathione and analogscysteine--1-D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (TIGR03447; EC 6.3.1.13; HMM-score: 28)
  • TheSEED:
  • PFAM:
    HUP (CL0039) tRNA-synt_1; tRNA synthetases class I (I, L, M and V) (PF00133; HMM-score: 794.1)
    DALR (CL0258) Anticodon_1; Anticodon-binding domain of tRNA (PF08264; HMM-score: 100.8)
    HUP (CL0039) tRNA-synt_1g; tRNA synthetases class I (M) (PF09334; HMM-score: 88.5)
    Zn_Beta_Ribbon (CL0167) zf-FPG_IleRS; Zinc finger found in FPG and IleRS (PF06827; HMM-score: 45.3)
    HUP (CL0039) tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 38.9)
    tRNA-synt_1_2; Leucyl-tRNA synthetase, Domain 2 (PF13603; HMM-score: 27.6)
    tRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 14.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:
  • protein partners:
    NWMN_RS0291550S ribosomal protein L1  [1] (data from MRSA252)
    NWMN_RS02965elongation factor Tu  [1] (data from MRSA252)
    NWMN_RS05380pyruvate dehydrogenase E1 component subunit alpha  [1] (data from MRSA252)
    NWMN_RS07455dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex  [1] (data from MRSA252)
    NWMN_RS08905isocitrate dehydrogenase (NADP(+))  [1] (data from MRSA252)
    NWMN_RS08930pyruvate kinase  [1] (data from MRSA252)
    NWMN_RS12080Asp23/Gls24 family envelope stress response protein  [1] (data from MRSA252)
    NWMN_RS1226030S ribosomal protein S9  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.148
    • Ymax_pos: 34
    • Cmax: 0.123
    • Cmax_pos: 34
    • Smax: 0.23
    • Smax_pos: 31
    • Smean: 0.146
    • D: 0.147
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446306836 NCBI
  • RefSeq: WP_000384691 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKNKGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIEQALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYEAAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFDVKDDKGVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNVNGEKYIIAEALSDAVAEALDWDKASIKLEKEYTGKELEYVVAQHPFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDDYIVGQKYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITHSYPHDWRTKKPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGEWVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAKDLLPEGFTHPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNSSITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSSTDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNINDFNPDTDSIPESELLEVDRYLLNRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRSMQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKWRTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQLFIVSQVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRCQQVVKSLV

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS06245 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]