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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS07615 [old locus tag: NWMN_1350 ]
  • pan locus tag?: SAUPAN003894000
  • symbol: NWMN_RS07615
  • pan gene symbol?:
  • synonym:
  • product: 5'-3' exonuclease

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS07615 [old locus tag: NWMN_1350 ]
  • symbol: NWMN_RS07615
  • product: 5'-3' exonuclease
  • replicon: chromosome
  • strand: -
  • coordinates: 1513570..1514448
  • length: 879
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (1513570..1514448) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
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    361
    421
    481
    541
    601
    661
    721
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    841
    ATGCCTAATAAAATATTACTTGTAGATGGTATGGCGCTATTATTTAGACATTTCTATGCT
    ACAAGTCTTCATAAACAATTTATGTACAATTCACAAGGTGAACCTACAAATGGAATACAA
    GGATTTGTGCGTCATATCTTTTCGGCAATACATGAAATACGCCCTACACATGTAGCTGTA
    TGTTGGGATATGGGACAATCAACTTTTAGAAATGATATGTTTGATGGTTATAAGCAAAAT
    CGTTCTGCACCACCAGAAGAATTGATACCACAATTTGATTATGTTAAAGAAATTTCAGAG
    CAATTTGGCTTTGTAAATATTGGCGTTAAAAACTATGAAGCGGATGATGTTATAGGTACA
    TTAGCACAACAATATTCAACTGATAACGATGTCTATATTATTACGGGCGACAAAGATTTA
    CTGCAATGTATTAATGACAATGTTGAAGTTTGGCTAATTAAAAAAGGTTTTAACATTTAT
    AATAGATATACATTACATCGTTTTAACGAAGAATATGCCCTTGAACCACAACAACTAATC
    GATATTAAAGCGTTTATGGGTGATACAGCAGATGGATATGCGGGTGTTAAAGGTATTGGC
    GAGAAAACGGCTATTAAGTTAATTCAGCAATATCAAAGCGTTGAAAATGTGGTTGAAAAC
    ATTGATGCATTATCAGCTGGGCAACGTAATAAGATTAATGACAATTTAGATGAACTATAT
    TTATCGAAGCGACTGGCTGAAATTCACACACAAGTACCAATTGATAGTGAAGCGCTTTTT
    GAGAAAATGTCATTTGCAACAACGCTTAATCATATTTTATCAATTTGTAACGAACATGAA
    TTACATGTTTCAGGAAAATATATTTCATCCCATTTTTAA
    60
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Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS07615 [old locus tag: NWMN_1350 ]
  • symbol: NWMN_RS07615
  • description: 5'-3' exonuclease
  • length: 292
  • theoretical pI: 5.04103
  • theoretical MW: 33401.5
  • GRAVY: -0.321918

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA polymerase I (TIGR00593; EC 2.7.7.7; HMM-score: 266.7)
    flap structure-specific endonuclease (TIGR03674; EC 3.1.-.-; HMM-score: 32)
    MetabolismAmino acid biosynthesisPyruvate familythreonine dehydratase (TIGR02079; EC 4.3.1.19; HMM-score: 14.8)
  • TheSEED:
  • PFAM:
    PIN (CL0280) 5_3_exonuc_N; 5'-3' exonuclease, N-terminal resolvase-like domain (PF02739; HMM-score: 176.5)
    5_3_exonuc_C (CL0464) 5_3_exonuc; 5'-3' exonuclease, C-terminal SAM fold (PF01367; HMM-score: 94)
    HHH (CL0198) HHH_5; Helix-hairpin-helix domain (PF14520; HMM-score: 16.3)
    HHH_2; Helix-hairpin-helix motif (PF12826; HMM-score: 15.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.148
    • Ymax_pos: 25
    • Cmax: 0.151
    • Cmax_pos: 34
    • Smax: 0.295
    • Smax_pos: 6
    • Smean: 0.204
    • D: 0.17
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 447055755 NCBI
  • RefSeq: WP_001133011 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGEPTNGIQGFVRHIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQFGFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIYNRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVVENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSICNEHELHVSGKYISSHF

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS07615 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]