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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS08235 [old locus tag: NWMN_1460 ]
  • pan locus tag?: SAUPAN004138000
  • symbol: NWMN_RS08235
  • pan gene symbol?: nfo
  • synonym:
  • product: endonuclease

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS08235 [old locus tag: NWMN_1460 ]
  • symbol: NWMN_RS08235
  • product: endonuclease
  • replicon: chromosome
  • strand: -
  • coordinates: 1627026..1627916
  • length: 891
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (1627026..1627916) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
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    481
    541
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    841
    ATGTTATTAGGATCACATGTTTCAATGAGTGGTAAAAAGATGTTAGAAGGTTCTGCTATA
    GAAGCGCATGAATATGGTGAAACAACATTTATGATTTATACTGGTGCACCTCAAAACACG
    CGCCGTAAAAGTATTGAAGATTTAAATATAACTAAAGGTCATGAAGTGATGGAAAAATAT
    GGTTTATCTAATATTGTTGTTCATGCACCATACATCATTAATATTGCAAATACAACCAAA
    CCAGAAACGTTTAATCTTGGTGTAGATTTCTTGCAACAAGAAATTGAAAGAACGCAAGCT
    ATAGGGGCTAAAGATATTGTATTACATCCAGGTGCGCATGTTGGAGCTGGTGTTGATGCC
    GGAATTAATAAGATTATTGAAGGATTAAATGAAGTTTTAACGAATGACAATAATGTACGT
    ATTGCGCTTGAAACGATGGCAGGTAAAGGTACAGAGATTGGTCGCTCATTTGAAGAGTTA
    GCACGTATTATTGATGGAGTTCATAATAACGAACGTTTATCAGTATGTTTTGATACATGT
    CATACGCATGATGCTGGATATAATGTTAAAGAGGACTTTGATGGCGTATTAAATGAATTT
    GATAAAATCATTGGAGTCGACAGAATCAAAGTTGTTCATGTCAATGATTCTAAAAATGAC
    CGTGGCGCTCAAAAAGACCGACATGAAAATATTGGCTTCGGTTACATTGGTTTTGATGCA
    TTAAATTACATTGTGCATCATGATAGTTTTAAAGATATTCCGAAAATCTTAGAAACACCT
    TATGTTGGTGAAGATAAGAAAAATAAAAAACCGCCATATAAACTTGAAATTGAAATGTTA
    AAACAACAGCAATTTGACCCAGAATTAAAAAATAAGGTTATGCAACAATAA
    60
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Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS08235 [old locus tag: NWMN_1460 ]
  • symbol: NWMN_RS08235
  • description: endonuclease
  • length: 296
  • theoretical pI: 5.89598
  • theoretical MW: 33158.3
  • GRAVY: -0.471284

Function[edit source | edit]

  • reaction:
    EC 3.1.21.2?  ExPASy
    Deoxyribonuclease IVEndonucleolytic cleavage to 5'-phosphooligonucleotide end-products
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairapurinic endonuclease (APN1) (TIGR00587; HMM-score: 331.8)
  • TheSEED:
  • PFAM:
    TIM_barrel (CL0036) AP_endonuc_2; Xylose isomerase-like TIM barrel (PF01261; HMM-score: 131.9)
    no clan definedUDG; Uracil DNA glycosylase superfamily (PF03167; HMM-score: 19.3)
    TFIIA_gamma_N; Transcription initiation factor IIA, gamma subunit, helical domain (PF02268; HMM-score: 12.6)
    DASH_Dad1; DASH complex subunit Dad1 (PF08649; HMM-score: 11.5)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.186
    • Ymax_pos: 11
    • Cmax: 0.187
    • Cmax_pos: 23
    • Smax: 0.453
    • Smax_pos: 3
    • Smean: 0.327
    • D: 0.241
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446846958 NCBI
  • RefSeq: WP_000924214 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MLLGSHVSMSGKKMLEGSAIEAHEYGETTFMIYTGAPQNTRRKSIEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIGAKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEELARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSKNDRGAQKDRHENIGFGYIGFDALNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLEIEMLKQQQFDPELKNKVMQQ

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS08235 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]