From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS08305 [old locus tag: NWMN_1474 ]
  • pan locus tag?: SAUPAN004153000
  • symbol: NWMN_RS08305
  • pan gene symbol?: phoH
  • synonym:
  • product: PhoH family protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS08305 [old locus tag: NWMN_1474 ]
  • symbol: NWMN_RS08305
  • product: PhoH family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1640719..1641666
  • length: 948
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_009641 (1640719..1641666) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1474

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGCCTGGAATTATACAAATAGACGATATGAACCAATCTCAAGCTTTAATTGGAAATAAT
    GATGAACATTTAAAAGCAATTGAAGAGAGTTTCGATGTTGTCATCCATGCAAGAGGACAA
    GAAGTTGCCGTTAAAGGTACAAAAATAGAAAACGTAGAAAAAGCGGAATCAGTATTAATC
    AATTTGCTGAAGGTTATTGATTTAGGTAATAATATTACAATTAAAGATGTTGAAGCAGCT
    ATTAAAATGGCGCATAATAACACAATTCAACATCTGTTAGATTTATATGATGAAGAGATA
    ACTAAAGATGCATTTGGTAAGACGATTCGTGCGAAAACGATGGGGCAACGTATATATGTT
    AATGCCATGAAAAATAATGATTTAGTATTTGGTATAGGTCCTGCTGGTACAGGTAAGACA
    TTCTTAGCTGTAGTTTATGCAGCAAAGCAACTCCGTAAAGGTGCTGTTAAACGTATTGTA
    TTAACAAGACCTGCTGTTGAAGCAGGAGAGTCACTTGGATTTTTACCAGGAGATTTGAAA
    GAAAAGGTAGATCCATATTTAAGACCTTTATATGATGGTCTATATACTGTTCTTGGGCGT
    GAACAAACAGAGCGATTTATTGAAAGAGGCATTATCGAAATAGCGCCACTTGCATATATG
    CGCGGACGAACATTAGAAGATGCATTTGTAATTCTTGATGAGGCGCAGAATACGACACAT
    GCGCAAATGAAAATGTTTTTAACAAGACTAGGTTTTGGCTCAAAAATGGTAGTTACTGGT
    GACCAAACTCAAATCGATTTACCTAAAGGTGTTAAAAGTGGACTTAAGGAAGCGGTCAGT
    AGGTTACACAACGTTAAAGGTATAAGTATATTGAAATTAGATCAGAGCGATGTAGTCAGA
    CATCCATTGGTAAGTAAGATCATTGAACATTATGAAGGAGAGAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    948

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_RS08305 [old locus tag: NWMN_1474 ]
  • symbol: NWMN_RS08305
  • description: PhoH family protein
  • length: 315
  • theoretical pI: 6.98088
  • theoretical MW: 34913.1
  • GRAVY: -0.165714

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair putative DNA helicase (TIGR00376; EC 3.6.4.12; HMM-score: 17)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    P-loop_NTPase (CL0023) PhoH; PhoH-like protein (PF02562; HMM-score: 314.7)
    and 10 more
    AAA_30; AAA domain (PF13604; HMM-score: 35)
    AAA_19; AAA domain (PF13245; HMM-score: 24.8)
    AAA_22; AAA domain (PF13401; HMM-score: 18.9)
    KH (CL0007) KH_1; KH domain (PF00013; HMM-score: 18)
    P-loop_NTPase (CL0023) IstB_IS21; IstB-like ATP binding protein (PF01695; HMM-score: 16.8)
    UvrD-helicase; UvrD/REP helicase N-terminal domain (PF00580; HMM-score: 13.9)
    DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 13.5)
    Zot; Zonular occludens toxin (Zot) (PF05707; HMM-score: 13.5)
    no clan defined Vps16_C; Vps16, C-terminal region (PF04840; HMM-score: 12.7)
    P-loop_NTPase (CL0023) AAA_25; AAA domain (PF13481; HMM-score: 12.3)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002182
    • TAT(Tat/SPI): 0.000143
    • LIPO(Sec/SPII): 0.000319
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAVKGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITKDAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGAVKRIVLTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLAYMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLKEAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]