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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS09070 [old locus tag: NWMN_1617 ]
  • pan locus tag?: SAUPAN004379000
  • symbol: NWMN_RS09070
  • pan gene symbol?: serA
  • synonym:
  • product: D-3-phosphoglycerate dehydrogenase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS09070 [old locus tag: NWMN_1617 ]
  • symbol: NWMN_RS09070
  • product: D-3-phosphoglycerate dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 1794725..1796329
  • length: 1605
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (1794725..1796329) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
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    1561
    ATGAAGCAATTTAATGTACTCGTTGCAGATCCCATATCAAAAGATGGTATCAAAGCATTA
    TTAGATCACGAACAATTCAATGTAGATATTCAAACTGGCTTGTCCGAAGAAGCATTAATC
    AAAATTATACCTTCATACCATGCTTTAATCGTTCGTAGTCAAACTACGGTTACTGAAAAT
    ATCATAAATGCTGCTGATTCTTTAAAAGTAATCGCACGCGCCGGTGTTGGTGTAGATAAT
    ATTAATATTGATGCTGCAACATTAAAAGGTATTTTAGTTATTAATGCCCCAGATGGTAAT
    ACGATTTCAGCTACTGAACATACACTGGCAATGTTATTATCAATGGCACGAAATATTCCG
    CAAGCACACCAATCACTTACAAATAAAGAATGGAATCGAAATGCATTTAAAGGTACTGAG
    CTTTATCATAAAACATTAGGTGTCATTGGTGCTGGTAGAATTGGTTTAGGTGTTGCTAAA
    CGTGCGCAAAGTTTCGGAATGAAAATACTAGCTTTTGACCCTTACTTAACGGATGAAAAA
    GCAAAATCTTTAAGCATTACGAAGGCAACAGTTGATGAGATTGCCCAACATTCTGATTTC
    GTTACATTACATACACCACTAACACCTAAAACAAAAGGCTTAATTAATGCTGTCTTTTTT
    GCCAAAGCAAAACCTAGTTTGCAAATAATCAATGTGGCACGTGGTGGTATTATTGATGAA
    AAGGCGCTAATAAAAGCATTAGACGAAGGACAAATTAGTCGGGCAGCTATCGATGTGTTT
    GAACATGAACCTGCAACTGACTCGCCTCTTGTTGCACATGATAAAATTATTGTTACACCT
    CATTTGGGTGCTTCAACAGTCGAAGCTCAAGAAAAAGTGGCAATTTCTGTTTCAAATGAA
    ATCATCGAAATTTTAATTGATGGTACTGTAACGCATGCAGTGAATGCACCTAAAATGGAC
    TTAAGCAATATAGATGATACTGTAAAATCATTCATCAATTTAAGCCAAACAGTTGGTGAA
    TTAGCTATTCAATTAATGTACAATGCACCAAGCTCTATTAAAATTACGTACGGTGGCGAC
    TTAGCCTCTATTGATAGTAGTTTATTAACACGTACAATTATTACTCATATTTTAAAAGAT
    GATCTTGGTCCTGAAGTCAATATTATCAATGCTCTAATGTTGTTAAATCAACAACAAGTG
    ACATTAAATATTGAAAATAATAAAGCAGAGACAGGTTTTAGTAACTACTTAGAGGTAGAA
    CTATCAAACGATAGCGATTCCGTTAAAGTTGGCGCTTCTGTCTTTACAGGTTTCGGTCCA
    AGAATTGTTAGAATTAATAATTTTTCTGTAGACTTAAAGCCAAATCAATATCAAATTGTG
    TCATATCATAATGATACTCCAGGTATGGTAGGAAAAACTGGCGCATTGTTAGGTAAATAC
    AATATCAACATTGCATCTATGACTTTAGGTAGAACTGAAGCGGGCGGAGATGCGCTAATG
    ATTTTATCCGTTGATCAACCTGTTTCAAACAATATAATTGATGAACTTAAACAAGTTGGT
    GAATACAATCAAATTTTCACAACTGAATTGACGGTACAGTCATAA
    60
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    1605

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS09070 [old locus tag: NWMN_1617 ]
  • symbol: NWMN_RS09070
  • description: D-3-phosphoglycerate dehydrogenase
  • length: 534
  • theoretical pI: 5.30951
  • theoretical MW: 57618.4
  • GRAVY: 0.0337079

Function[edit source | edit]

  • reaction:
    EC 1.1.1.95?  ExPASy
    Phosphoglycerate dehydrogenase3-phospho-D-glycerate + NAD+ = 3-phosphonooxypyruvate + NADH2-hydroxyglutarate + NAD+ = 2-oxoglutarate + NADH
  • TIGRFAM:
    MetabolismAmino acid biosynthesisSerine familyphosphoglycerate dehydrogenase (TIGR01327; EC 1.1.1.95; HMM-score: 745.7)
    MetabolismEnergy metabolismAmino acids and aminesL-serine dehydratase, iron-sulfur-dependent, beta subunit (TIGR00719; EC 4.3.1.17; HMM-score: 38.8)
    MetabolismEnergy metabolismGlycolysis/gluconeogenesisL-serine dehydratase, iron-sulfur-dependent, beta subunit (TIGR00719; EC 4.3.1.17; HMM-score: 38.8)
  • TheSEED:
  • PFAM:
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 209.9)
    Form_Glyc_dh (CL0325) 2-Hacid_dh; D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain (PF00389; HMM-score: 119.2)
    ACT (CL0070) ACT; ACT domain (PF01842; HMM-score: 38.6)
    NADP_Rossmann (CL0063) NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 18.7)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 16.9)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 14.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.15
    • Ymax_pos: 65
    • Cmax: 0.23
    • Cmax_pos: 65
    • Smax: 0.174
    • Smax_pos: 19
    • Smean: 0.092
    • D: 0.128
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446731589 NCBI
  • RefSeq: WP_000808845 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIPSYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNINIDAATLKGILVINAPDGNTISATEHTLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAKRAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAVFFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKIIVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINLSQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINALMLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSVDLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPVSNNIIDELKQVGEYNQIFTTELTVQS

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS09070 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]