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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS12445 [old locus tag: NWMN_2156 ]
  • pan locus tag?: SAUPAN005706000
  • symbol: NWMN_RS12445
  • pan gene symbol?: topB
  • synonym:
  • product: DNA topoisomerase III

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS12445 [old locus tag: NWMN_2156 ]
  • symbol: NWMN_RS12445
  • product: DNA topoisomerase III
  • replicon: chromosome
  • strand: -
  • coordinates: 2378252..2380387
  • length: 2136
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (2378252..2380387) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
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    481
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    901
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    1021
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    1141
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    ATGAAATCTTTAATATTAGCTGAAAAACCATCAGTCGCAAGAGATATTGCTGATGCTTTA
    CAAATAAATCAGAAGCGTAATGGTTACTTTGAAAATAACCAATATATTGTCACGTGGGCG
    TTAGGTCATCTAGTGACAAATGCGACACCTGAACAATACGATAAAAATTTAAAGGAATGG
    CGATTAGAAGACCTTCCAATTATACCTAAATATATGAAAACTGTTGTTATTGGTAAAACA
    AGCAAACAATTTAAAACAGTAAAAGCGTTAATTTTAGATAATAAAGTGAAAGATATTATT
    ATTGCAACAGATGCTGGACGAGAAGGTGAACTAGTTGCAAGATTGATTTTGGATAAAGTT
    GGTAACAAAAAGCCAATCCGTCGATTATGGATTAGCTCAGTTACTAAAAAAGCTATTCAA
    CAAGGTTTTAAAAATTTAAAAGACGGTCGTCAATATAACGATTTGTATTATGCAGCGTTA
    GCGAGAAGTGAGGCAGATTGGATTGTTGGGATTAATGCAACGCGTGCACTAACAACAAAG
    TATGATGCACAGCTATCCCTGGGACGTGTTCAGACACCAACGATTCAATTAGTAAATACA
    CGACAACAAGAGATTAATCAGTTCAAACCACAACAATACTTTACATTATCATTAACGGTA
    AAAGGGTTTGATTTTCAGCTAGAATCAAATCAGCGATATACCAATAAAGAAACTTTAGAA
    CAGATGGTTAATAATTTGAAAAATGTCGATGGTAAGATTAAATCTGTTGCTACTAAACAT
    AAGAAGTCGTATCCGCAATCACTGTACAATTTAACAGATTTACAACAAGATATGTATAGA
    CGTTATAAAATTGGACCTAAAGAAACATTGAATACACTTCAAAGCTTATATGAGAGACAT
    AAAGTCGTAACCTATCCAAGAACAGATTCAAACTATTTAACAACTGATATGGTAGATACT
    ATGAAAGAACGTATTCAGGCGACGATGGCAACAACATATAAAGACCAAGCACGCCCATTA
    ATGTCTAAAACATTTTCATCAAAAATGTCGATATTTAATAATCAAAAAGTATCTGATCAC
    CATGCAATTATTCCTACAGAAGTGAGACCTGTCATGTCAGACTTAAGTAATAGAGAATTA
    AAGTTATACGATATGATTGTCGAGCGTTTTTTAGAAGCTTTAATGCCTCCGCACGAGTAT
    GACGCGATAACTGTAACTTTAGAGGTTGCAGGGCACACATTTGTTTTGAAAGAGAATGTA
    ACAACTGTTTTAGGTTTTAAATCTATTAGACAAGGTGAATCTATTACAGAGATGCAACAG
    CCTTTTTCAGAAGGCGATGAAGTGAAGATTTCAAAAACAAATATTAGAGAACATGAAACA
    ACACCTCCAGAATATTTTAATGAAGGTTCGTTATTAAAAGCGATGGAGAACCCTCAGAAC
    TTTATTCAATTGAAGGATAAAAAATATGCGCAAACTTTAAAACAAACAGGTGGTATCGGC
    ACAGTTGCAACAAGGGCCGACATTATCGATAAATTATTTAATATGAATGCCATTGAATCA
    AGAGACGGTAAAATTAAAGTAACGTCAAAAGGTAAACAAATATTAGAATTAGCACCAGAA
    GAATTAACGTCGCCACTTTTAACTGCACAATGGGAAGAAAAATTACTTTTAATTGAACGT
    GGTAAATATCAGGCGAAAACATTTATTAATGAAATGAAAGATTTTACGAAAGATGTTGTA
    AATGGGATTAAAAATAGTGATCGTAAATATAAACACGATAATTTAACAACCACAGAATGC
    CCAACGTGTGGTAAATTCATGATTAAAGTTAAAACTAAAAATGGTCAGATGCTTGTGTGC
    CAAGATCCATCTTGTAAGACGAAAAAGAATGTACAGCGCAAAACAAATGCAAGATGTCCA
    AACTGTAAAAAGAAATTAACGTTGTTTGGTAAAGGGAAAGAAGCGGTATATCGTTGTGTT
    TGTGGACATTCTGAAACGCAAGCACATATGGATCAGCGTATGAAGTCTAAATCCTCTGGT
    AAAGTATCTCGTAAAGAAATGAAAAAGTATATGAATAAAAATGAAGGTTTAGACAATAAT
    CCGTTTAAAGATGCATTAAAGAACTTGAATTTATAG
    60
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    2136

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS12445 [old locus tag: NWMN_2156 ]
  • symbol: NWMN_RS12445
  • description: DNA topoisomerase III
  • length: 711
  • theoretical pI: 10.0831
  • theoretical MW: 81523.4
  • GRAVY: -0.657806

Function[edit source | edit]

  • reaction:
    EC 5.99.1.2?  ExPASy
    DNA topoisomeraseATP-independent breakage of single-stranded DNA, followed by passage and rejoining
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA topoisomerase III (TIGR01056; EC 5.99.1.2; HMM-score: 674.4)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA topoisomerase I (TIGR01057; EC 5.99.1.2; HMM-score: 262.4)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA topoisomerase I (TIGR01051; EC 5.99.1.2; HMM-score: 206.7)
    conserved hypothetical protein (TIGR02443; HMM-score: 14.8)
    Genetic information processingProtein fateProtein modification and repairhydrogenase nickel insertion protein HypA (TIGR00100; HMM-score: 7.2)
  • TheSEED:
  • PFAM:
    no clan definedTopoisom_bac; DNA topoisomerase (PF01131; HMM-score: 371.4)
    Toprim-like (CL0413) Toprim; Toprim domain (PF01751; HMM-score: 57.3)
    Zn_Beta_Ribbon (CL0167) zf-C4_Topoisom; Topoisomerase DNA binding C4 zinc finger (PF01396; HMM-score: 18.2)
    DUF2387; Probable metal-binding protein (DUF2387) (PF09526; HMM-score: 14.2)
    zf-ribbon_3; zinc-ribbon domain (PF13248; HMM-score: 14)
    Toprim-like (CL0413) Toprim_4; Toprim domain (PF13662; HMM-score: 13)
    no clan definedPXA; PXA domain (PF02194; HMM-score: 12.7)
    DUF5416; Family of unknown function (DUF5416) (PF17437; HMM-score: 12.2)
    4H_Cytokine (CL0053) Leptin; Leptin (PF02024; HMM-score: 11.1)
    no clan definedDNA_ligase_ZBD; NAD-dependent DNA ligase C4 zinc finger domain (PF03119; HMM-score: 10.1)
    HypA; Hydrogenase/urease nickel incorporation, metallochaperone, hypA (PF01155; HMM-score: 9.8)
    Zn_Beta_Ribbon (CL0167) zinc-ribbons_6; zinc-ribbons (PF07191; HMM-score: 9.5)
    zf-TFIIB; Transcription factor zinc-finger (PF13453; HMM-score: 7.1)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.143
    • Ymax_pos: 20
    • Cmax: 0.155
    • Cmax_pos: 54
    • Smax: 0.213
    • Smax_pos: 17
    • Smean: 0.166
    • D: 0.152
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446761223 NCBI
  • RefSeq: WP_000838479 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHLVTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIATDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAALARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSLTVKGFDFQLESNQRYTNKETLEQMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQDMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMATTYKDQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLEALMPPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKISKTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADIIDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAKTFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPSCKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKVSRKEMKKYMNKNEGLDNNPFKDALKNLNL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS12445 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]