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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS14130 [old locus tag: NWMN_2459 ]
  • pan locus tag?: SAUPAN006223000
  • symbol: NWMN_RS14130
  • pan gene symbol?:
  • synonym:
  • product: lactate dehydrogenase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS14130 [old locus tag: NWMN_2459 ]
  • symbol: NWMN_RS14130
  • product: lactate dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 2705648..2706646
  • length: 999
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (2705648..2706646) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
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    481
    541
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    721
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    841
    901
    961
    ATGACAAAAATTAAAATCATGAGTGTACGTGATGAAGATATGCCTTATATCAAGGCGTGG
    GCAGAGAAACATCATGTTGAGGTAGATATTACTAAAGAAGCATTAACTGATGACAATGTT
    GAAGGTGTCGCTGGTTATGATGGCTTATCGCTATCACAACAAATACCATTATCAGAGCAT
    GTTTATAAACGATTAAATGAACTAGGTATTAAACAAATCGCACAACGTAGTGCTGGATTT
    GATACATATGATTTGGAGTTAGCAAACAAGTATAACTTTATTGTGTCAAATGTGCCGTCA
    TACTCTCCGAATTCAATTGCGGAGTTTGCTGTGAACCAGGCTATTAATGTGGTTCGTCAT
    TTTAATCAAATTCAAACGAAAGTGCGGGAGCATGATTTTAGATGGGAACCTACTATTTTA
    TCGAAATCCATTAAAGATTTAAAAGTAGCTGTTATTGGTACAGGTCGAATTGGCCGTGTA
    GTAGCCGATATATTTGCCAATGGTTATCAAAGTGATGTGGTCGCATACGACCCGTTTCCT
    AATGCTAAAATTGCAACGTATGTCGATTACAAAGATACGATTGAGGAAGCGGTTGAAGGT
    GCTGATATCGTGACATTACATGTACCTGCAACGAAATATAATCATTATCTATTTAATGCT
    GAATTATTTAAACATTTTAAAAAGGGCGCAGTATTTGTCAATTGTGCGAGAGGTTCTTTA
    GTAGATACTAAGGCGTTATTAGACGCATTAGACAATGGTGTGATTAAAGGTGCAGCACTT
    GATACGTATGAATTTGAACGCAAACTTTTCCCAAGTGATCAAAGGGGCAAAACACTGAAC
    GATCCATTGTTAGAATCGTTGATTGACAGGGAAGATGTCATATTAACACCACATATTGCG
    TTTTATACTGAAGCTGCAGTTAAAAATCTAATTGTCGATGCATTAGATGCAACATTAGAT
    GTATTGCAGACTGGAGATACTAGGTTACGAGTAAATTAA
    60
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    999

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS14130 [old locus tag: NWMN_2459 ]
  • symbol: NWMN_RS14130
  • description: lactate dehydrogenase
  • length: 332
  • theoretical pI: 5.25933
  • theoretical MW: 37222.9
  • GRAVY: -0.196386

Function[edit source | edit]

  • reaction:
    EC 1.1.1.28?  ExPASy
    D-lactate dehydrogenase(R)-lactate + NAD+ = pyruvate + NADH
  • TIGRFAM:
    MetabolismAmino acid biosynthesisSerine familyphosphoglycerate dehydrogenase (TIGR01327; EC 1.1.1.95; HMM-score: 167.6)
    Cellular processesCellular processesSporulation and germinationdipicolinic acid synthetase, A subunit (TIGR02853; HMM-score: 18)
    2-hydroxy-3-oxopropionate reductase (TIGR01505; EC 1.1.1.60; HMM-score: 16.7)
    glycerol-3-phosphate dehydrogenase (NAD(+)) (TIGR03376; EC 1.1.1.8; HMM-score: 14)
    MetabolismEnergy metabolismSugarsinositol 2-dehydrogenase (TIGR04380; EC 1.1.1.18; HMM-score: 14)
    glutamate synthase, small subunit (TIGR01318; HMM-score: 12.6)
    MetabolismEnergy metabolismAmino acids and amines3-hydroxyisobutyrate dehydrogenase (TIGR01692; EC 1.1.1.31; HMM-score: 12.2)
  • TheSEED:
  • PFAM:
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 172.2)
    Form_Glyc_dh (CL0325) 2-Hacid_dh; D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain (PF00389; HMM-score: 100.5)
    NADP_Rossmann (CL0063) NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 29)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 23)
    NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 21.2)
    IlvN; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 20.9)
    HUP (CL0039) UDPG_MGDP_dh_C; UDP-glucose/GDP-mannose dehydrogenase family, UDP binding domain (PF03720; HMM-score: 18.7)
    NADP_Rossmann (CL0063) Gp_dh_N; Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain (PF00044; HMM-score: 18.1)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 17.8)
    AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 16.5)
    Form_Glyc_dh (CL0325) DpaA_N; Dipicolinate synthase subunit A N-terminal domain (PF16924; HMM-score: 14.8)
    NADP_Rossmann (CL0063) GFO_IDH_MocA; Oxidoreductase family, NAD-binding Rossmann fold (PF01408; HMM-score: 14.7)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 14)
    Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 13.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.112
    • Ymax_pos: 22
    • Cmax: 0.122
    • Cmax_pos: 22
    • Smax: 0.155
    • Smax_pos: 18
    • Smean: 0.092
    • D: 0.104
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446083503 NCBI
  • RefSeq: WP_000161358 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVAGYDGLSLSQQIPLSEHVYKRLNELGIKQIAQRSAGFDTYDLELANKYNFIVSNVPSYSPNSIAEFAVNQAINVVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRVVADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLFNAELFKHFKKGAVFVNCARGSLVDTKALLDALDNGVIKGAALDTYEFERKLFPSDQRGKTLNDPLLESLIDREDVILTPHIAFYTEAAVKNLIVDALDATLDVLQTGDTRLRVN

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS14130 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]