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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS14300 [old locus tag: NWMN_2492 ]
  • pan locus tag?: SAUPAN006275000
  • symbol: NWMN_RS14300
  • pan gene symbol?:
  • synonym:
  • product: TetR/AcrR family transcriptional regulator

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS14300 [old locus tag: NWMN_2492 ]
  • symbol: NWMN_RS14300
  • product: TetR/AcrR family transcriptional regulator
  • replicon: chromosome
  • strand: -
  • coordinates: 2737251..2737838
  • length: 588
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (2737251..2737838) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
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    541
    ATGACTATAGATCGACGTATTAGAAAATCACAAGTTGCAATGAGAAATGCATTTATTGAA
    CTTTTACATCAACACCAATTAGAAGAGATTACTGTGCAACAAATTGCAGATTTAGCTGAT
    GTTAACCGTAGTACATTTTATACGCATTATTATGATAAGTATGATTTGCTTGAAAAATTA
    GAGGACCAGCAGTTGGAGGAAATAAGAGACTTCATTCATGATGAGAAGTTAAATGGAGCA
    GTTAAGTTATCCACGGATAACATACATCAAATTATGACCGTTTTAATAGAAATGATAGAA
    AAAGACATCGCATTTTATCAATTGATGTTTCGTATGGGTAAAGATTCAAATATCCACGAA
    AAACTTTATGATTTAATTATGTGTCATTTACAACGTTATAAAAATGAAAATGATCAAATT
    AATGGTATACCATTTTCTTATTTCATGAGTTATGTTTCAGGTGCGGGATTGTCTTTTATA
    AGACATTGGGTAGAAGATCCAAACCGTATAGACAAAGTTGACTTAATAAGGCATTTTAAT
    ACGATTGTCAATCAAGGGCCAGCAACGATAATTAAAAATATACAATAA
    60
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    588

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS14300 [old locus tag: NWMN_2492 ]
  • symbol: NWMN_RS14300
  • description: TetR/AcrR family transcriptional regulator
  • length: 195
  • theoretical pI: 6.02724
  • theoretical MW: 23193.4
  • GRAVY: -0.404103

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    probable dihydroxyacetone kinase regulator (TIGR02366; HMM-score: 42.8)
    Signal transductionRegulatory functionsDNA interactionsmycofactocin system transcriptional regulator (TIGR03968; HMM-score: 25.9)
    Signal transductionRegulatory functionsDNA interactionstranscriptional repressor BetI (TIGR03384; HMM-score: 21.1)
    sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03088; HMM-score: 15.6)
    pyrimidine utilization regulatory protein R (TIGR03613; HMM-score: 13.5)
  • TheSEED:
  • PFAM:
    no clan definedTetR_C_8; Transcriptional regulator C-terminal region (PF14278; HMM-score: 38.7)
    HTH (CL0123) TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 27.7)
    HTH_Tnp_1_2; Helix-turn-helix of insertion element transposase (PF13022; HMM-score: 16.8)
    HTH_23; Homeodomain-like domain (PF13384; HMM-score: 14.7)
    no clan definedTim17; Tim17/Tim22/Tim23/Pmp24 family (PF02466; HMM-score: 13.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.157
    • Ymax_pos: 11
    • Cmax: 0.113
    • Cmax_pos: 39
    • Smax: 0.34
    • Smax_pos: 1
    • Smean: 0.232
    • D: 0.186
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446073257 NCBI
  • RefSeq: WP_000151112 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MTIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRSTFYTHYYDKYDLLEKLEDQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIEMIEKDIAFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSFIRHWVEDPNRIDKVDLIRHFNTIVNQGPATIIKNIQ

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS14300 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]