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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS14545 [old locus tag: NWMN_2531 ]
  • pan locus tag?: SAUPAN006361000
  • symbol: NWMN_RS14545
  • pan gene symbol?: arcC
  • synonym:
  • product: carbamate kinase 2

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS14545 [old locus tag: NWMN_2531 ]
  • symbol: NWMN_RS14545
  • product: carbamate kinase 2
  • replicon: chromosome
  • strand: -
  • coordinates: 2784347..2785288
  • length: 942
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (2784347..2785288) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAAGAGAAAATTGTCATTGCATTAGGCGGTAATGCGATACAGACAACAGAAGCAACA
    GCTGAAGCACAACAAACAGCTATTAGATGTGCGATGCAAAACCTTAAACCTTTATTTGAT
    TCACCAGCGCGTATTGTGATTTCACATGGTAATGGTCCACAAATTGGAAGTTTATTAATC
    CAACAAGCTAAATCGAACAGTGACACAACGCCGGCAATGCCATTGGATACTTGTGGTGCA
    ATGTCACAGGGTATGATAGGCTATTGGTTGGAAACTGAAATCAATCGCATTTTAACTGAA
    ATGAATAGTGATAGAACTGTAGGCACAATCGTTACACGTGTGGAAGTAGATAAAGATGAT
    CCACGATTTGATAACCCAACTAAACCAATTGGTCCTTTTTATACGAAAGAAGAAGTTGAA
    GAATTACAAAAAGAACAGCCAGACTCAGTCTTTAAAGAAGATGCAGGACGTGGTTATAGA
    AAAGTAGTTGCGTCACCACTACCTCAATCTATACTAGAACACCAGTTAATTCGAACTTTA
    GCAGACGGTAAAAATATTGTCATTGCATGCGGTGGTGGCGGTATTCCAGTTATAAAAAAA
    GAAAATACCTATGAAGGTGTTGAAGCGGTTATAGATAAAGATTTTGCTAGTGAGAAATTA
    GCAACGCTGATTGAAGCAGATACCTTAATGATTCTTACGAATGTAGAAAATGTATTTATT
    AACTTTAATGAACCTAATCAACAACAAATCGATGATATTGATGTAGCAACACTGAAAAAA
    TACGCGGCACAAGGTAAGTTTGTGGAAGGATCGATGTTGCCAAAAATAGAAGCTGCGATA
    CGATTTGTTGAAAGTGGGGAAAACAAAAAAGTTATCATTACCAATTTAGAGCAGGCATAC
    GAAGCTTTGATTGGTAATAAAGGTACACACATTCACATGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    942

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS14545 [old locus tag: NWMN_2531 ]
  • symbol: NWMN_RS14545
  • description: carbamate kinase 2
  • length: 313
  • theoretical pI: 4.68382
  • theoretical MW: 34328.9
  • GRAVY: -0.269649

Function[edit source | edit]

  • reaction:
    EC 2.7.2.2?  ExPASy
    Carbamate kinaseATP + NH3 + CO2 = ADP + carbamoyl phosphate
  • TIGRFAM:
    MetabolismEnergy metabolismAmino acids and aminescarbamate kinase (TIGR00746; EC 2.7.2.2; HMM-score: 392.4)
    MetabolismAmino acid biosynthesisGlutamate familyacetylglutamate kinase (TIGR00761; EC 2.7.2.8; HMM-score: 49.8)
    MetabolismAmino acid biosynthesisGlutamate familyglutamate 5-kinase (TIGR01027; EC 2.7.2.11; HMM-score: 25.5)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesNucleotide and nucleoside interconversionsputative uridylate kinase (TIGR02076; EC 2.7.4.-; HMM-score: 16.7)
    MetabolismAmino acid biosynthesisGlutamate familydelta l-pyrroline-5-carboxylate synthetase (TIGR01092; HMM-score: 11.7)
  • TheSEED:
  • PFAM:
    no clan definedAA_kinase; Amino acid kinase family (PF00696; HMM-score: 93.8)
    P-loop_NTPase (CL0023) SKI; Shikimate kinase (PF01202; HMM-score: 18.3)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.237
    • Ymax_pos: 22
    • Cmax: 0.213
    • Cmax_pos: 22
    • Smax: 0.46
    • Smax_pos: 18
    • Smean: 0.218
    • D: 0.229
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446582688 NCBI
  • RefSeq: WP_000660034 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKEKIVIALGGNAIQTTEATAEAQQTAIRCAMQNLKPLFDSPARIVISHGNGPQIGSLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMNSDRTVGTIVTRVEVDKDDPRFDNPTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYRKVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASEKLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFVEGSMLPKIEAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS14545 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]