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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0068 [new locus tag: SA_RS00490 ]
  • pan locus tag?: SAUPAN000456000
  • symbol: kdpA
  • pan gene symbol?: kdpA
  • synonym:
  • product: potassium-transporting ATPase subunit A

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0068 [new locus tag: SA_RS00490 ]
  • symbol: kdpA
  • product: potassium-transporting ATPase subunit A
  • replicon: chromosome
  • strand: +
  • coordinates: 77116..78792
  • length: 1677
  • essential: no DEG

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAGTATTGTGTTGTTTTTGATTGTATTTATCTTGCTCTCACTCATTGTGAGCCGATAT
    TTATATTCAGTTGCTTTAAATGTGCCATCTAAAATAGATGTTGTTTTTAATCCGATTGAG
    AAATTGATTTATCAACTGATTGGCACGAAATTAGAACACATGTCTGGGAAGACGTATATC
    AAACATTTTTTGTTGTTTAACGGATTGATGGGCGGATTGTCCTTTGTATTATTGCTTATT
    CAACAATGGCTGTTTTTGAATCCTAACCATAATTTAAATCAATCTGTATCGTTAGCCTTT
    AATACTATGGCATCTTTTTTGACCAATACTAACTTACAGCATTATGCAGGTGAAACAGAT
    TTAAGTTATTTAACACAAATGTGTGTCATCACTTTCTTAATGTTCACGTCAGCAGCGTCA
    GGTTACGCCGTATGTATTGCGATGTTAAGACGTTTGACTGGAATGACAGATGTGATTGGT
    AATTTCTATCAAGATATTACGCGTTTTATTGTACGGGTGCTCATACCTTTCGCATTGATC
    ATCAGTTTGTTTTTAATCAGTCAGGGCACACCGCAAACGCTTAAAGGTAATTTGGTGATT
    GAGACATTATCAGGTGTGAAACAAACGATTGCATATGGACCGATGGCGTCTTTAGAATCT
    ATTAAACATTTAGGGACAAATGGTGGTGGTTTCTTAGGTGCGAACTCTTCTACACCTTTT
    GAAAATCCGACATACTGGTCTAATTACGCTGAAGCTTTAAGTATGATGTTGATTCCAGGT
    TCATTAGTCTTTCTATTCGGTAGAATGTTGAAAACTAAACTACAGATTCATCCGCATGCG
    ATTATGATTTTCGTTGCGATGTTTGTAATGTTCATCGGCTTTTTAGTGACATGTCTCTAT
    TTTGAATTTGCGGGGAATCCAGTGTTGCATCACTTAGGTATTGCCGGTGGCAATATGGAA
    GGCAAAGAAACACGTTTCGGTATTGGCTTATCCGCTTTATTTACAACCATTACGACCGCT
    TTTACTACAGGAACAGTTAACAATATGCACGATAGTCTTACACCGCTAGGCGGCATGGTT
    CCAATGGTATTAATGATGTTGAATGCAGTTTTTGGCGGTGAAGGTGTTGGGCTGATGAAC
    ATGTTGATTTATGTCATGTTAACGGTCTTTATCTGTAGTTTGATGATTGGGAAAACACCA
    AGTTATTTAGGAATGAAGATTGAAGGTAAAGAGATGAAACTCATTGCGCTTTCTTTCTTA
    GTACATCCTTTACTTATTTTGGTTTTTTCAGCACTAGCTTTTATTGTGCCAGGGGCATCA
    GATGCGTTAACTAATCCGCAATTCCACGGTGTATCACAAGTGTTGTATGAGTTTACATCA
    TCTTCAGCGAATAATGGCTCTGGTTTTGAAGGATTAGGAGACAATACGGTATTTTGGAAC
    ATTTCAACAGGCATTGTGATGTTGCTTGCACGATATATTCCAATCGTTTTACAAATTTTG
    ATTGTATCTAGTTTGGTAAATAAAAAGACCTATCAGCAACATACTCAAGATGTACCGATT
    AATAATTTATTTTTCAGCAGTGTATTGATTATCTTTATTATTTTGTTGAGCGGCTTAACG
    TTCTTACCTGACTTAATGCTTGGACCAATAGGCGAACAGCTTTTGCTGCACGCATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1677

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0068 [new locus tag: SA_RS00490 ]
  • symbol: KdpA
  • description: potassium-transporting ATPase subunit A
  • length: 558
  • theoretical pI: 7.72916
  • theoretical MW: 61262.4
  • GRAVY: 0.72276

Function[edit source | edit]

  • reaction:
    EC 3.6.3.12?  ExPASy
    Potassium-transporting ATPaseATP + H2O + K+(Out) = ADP + phosphate + K+(In)
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundsK+-transporting ATPase, A subunit (TIGR00680; EC 3.6.3.12; HMM-score: 588.1)
  • TheSEED:  
    Potassium metabolismPotassium metabolism - no subcategoryPotassium homeostasis Potassium-transporting ATPase A chain (EC 3.6.3.12) (TC 3.A.3.7.1) 
  • PFAM:
    Ion_channel (CL0030) KdpA; Potassium-transporting ATPase A subunit (PF03814; HMM-score: 716.7)
    CopD_like (CL0430) DUF4149; Domain of unknown function (DUF4149) (PF13664; HMM-score: 14.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.16
    • Ymax_pos: 26
    • Cmax: 0.136
    • Cmax_pos: 33
    • Smax: 0.406
    • Smax_pos: 1
    • Smean: 0.196
    • D: 0.174
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSIVLFLIVFILLSLIVSRYLYSVALNVPSKIDVVFNPIEKLIYQLIGTKLEHMSGKTYIKHFLLFNGLMGGLSFVLLLIQQWLFLNPNHNLNQSVSLAFNTMASFLTNTNLQHYAGETDLSYLTQMCVITFLMFTSAASGYAVCIAMLRRLTGMTDVIGNFYQDITRFIVRVLIPFALIISLFLISQGTPQTLKGNLVIETLSGVKQTIAYGPMASLESIKHLGTNGGGFLGANSSTPFENPTYWSNYAEALSMMLIPGSLVFLFGRMLKTKLQIHPHAIMIFVAMFVMFIGFLVTCLYFEFAGNPVLHHLGIAGGNMEGKETRFGIGLSALFTTITTAFTTGTVNNMHDSLTPLGGMVPMVLMMLNAVFGGEGVGLMNMLIYVMLTVFICSLMIGKTPSYLGMKIEGKEMKLIALSFLVHPLLILVFSALAFIVPGASDALTNPQFHGVSQVLYEFTSSSANNGSGFEGLGDNTVFWNISTGIVMLLARYIPIVLQILIVSSLVNKKTYQQHTQDVPINNLFFSSVLIIFIILLSGLTFLPDLMLGPIGEQLLLHA

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]

T Ito, Y Katayama, K Hiramatsu
Cloning and nucleotide sequence determination of the entire mec DNA of pre-methicillin-resistant Staphylococcus aureus N315.
Antimicrob. Agents Chemother.: 1999, 43(6);1449-58
[PubMed:10348769] [WorldCat.org] (P p)