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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0208 [new locus tag: SA_RS01225 ]
  • pan locus tag?: SAUPAN001066000
  • symbol: SA0208
  • pan gene symbol?: malF
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0208 [new locus tag: SA_RS01225 ]
  • symbol: SA0208
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 246833..248101
  • length: 1269
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACGAAACGTAACCCTAAATTAGCGGCATTATTATCTGTTATACCTGGTTTGGGACAG
    TTTTATAATAAAAGACCCATTAAAGGTACGATATTTTTTATCTTTTTCATCAGTTTTATT
    TCTGTATTTTATAGCTTTTTAAATATTGGTTTTTGGGGATTGTTCACATTAGGGACAGTA
    CCTAAGTTAGACGATTCTCGTGTCTTACTTGCACAAGGTATTATTTCTATCTTACTCGTT
    GCTTTCGCAATCATGCTATATGTCATTAATATTTTAGATGCATATCGTAATGCTGAACGA
    TTTAATCGCAATGAGGAAATAAAGGATCCGAAGGCGCGTATGGTGGCAACATGGGACAAG
    ACGTTCCCATACTTACTAATCTCACCAGGTACATTCTTATTGATATTTGTAGTTGTATTT
    CCATTAATATTTATGTTTGGAGTAGCATTTACAAACTACAATTTATACAACGCGCCTCCG
    AGACACACATTAGAATGGGTTGGTTTAGATAACTTTAAAACGTTATTCACAATTGGCGTT
    TGGCGCAAAACATTTTTCAGTGTTATTACTTGGACATTAGTATGGACGCTTGTTGCAACG
    ACACTTCAAATTGCATTAGGGCTGTTTTTGGCAATTATTGTAAATCACCCTGTCGTCAAA
    GGTAAGAAATTTATCCGTACTGTGTTAATCCTACCTTGGGCTGTACCATCATTTGTGACA
    ATTTTAATATTTGTAGCGTTATTTAATGATGAATTTGGTGCGATAAATAATGATATTTTG
    CAACCTTTATTAGGTGTAGCACCAGCATGGTTAAGTGATCCGTTTTGGGCAAAAGTGGCA
    TTAATCGGCATTCAAGTATGGCTTGGATTCCCATTTGTCTTTGCACTGTTCACTGGAGTA
    CTGCAAAGTATTTCATCAGATTGGTACGAAGCAGCAGATATGGATGGTGCGTCTAGTTGG
    CAAAAGTTTAGAAACATCACATTCCCGCATGTCATTTACGCCACAGCGCCATTGTTAATT
    ATGCAATATGCAGGTAATTTCAATAATTTTAATCTTATTTATCTATTTAATAAAGGCGGT
    CCACCAGTGTCAGGGCAGAATGCTGGTAGTACAGATATCTTGATATCTTGGGTGTATAAT
    CTGACATTTGAGTTTAACAACTTCAACATGGGTGCAGTTGTGTCATTAATTATTGGATTT
    ATTGTTGCTATTGTCGCATTTATTCAATTCAGACGTACAAGTACGTTTAAAGATGAGGGA
    GGTTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1269

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0208 [new locus tag: SA_RS01225 ]
  • symbol: SA0208
  • description: hypothetical protein
  • length: 422
  • theoretical pI: 9.9436
  • theoretical MW: 47646.8
  • GRAVY: 0.659242

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesputative 2-aminoethylphosphonate ABC transporter, permease protein (TIGR03262; HMM-score: 61.6)
    MetabolismTransport and binding proteinsAnionssulfate ABC transporter, permease protein CysW (TIGR02140; HMM-score: 47.2)
    MetabolismTransport and binding proteinsAnionssulfate ABC transporter, permease protein (TIGR00969; HMM-score: 46.3)
    MetabolismTransport and binding proteinsAnionssulfate ABC transporter, permease protein CysT (TIGR02139; HMM-score: 46.3)
    MetabolismTransport and binding proteinsAnionsmolybdate ABC transporter, permease protein (TIGR02141; HMM-score: 36.2)
    NifC-like ABC-type porter (TIGR01581; HMM-score: 34.3)
    2-aminoethylphosphonate ABC transport system, membrane component PhnV (TIGR03255; HMM-score: 20.1)
    2-aminoethylphosphonate ABC transporter, permease protein (TIGR03226; HMM-score: 17.9)
    MetabolismTransport and binding proteinsAnionsphosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 16.4)
    Cellular processesCellular processesChemotaxis and motilityflagellar biosynthetic protein FlhB (TIGR00328; HMM-score: 11.8)
  • TheSEED:  
    Maltose and Maltodextrin Utilization Maltodextrin ABC transporter, permease protein MdxF 
  • PFAM:
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 91.1)
    no clan definedTM_PBP2_N; N-terminal of TM subunit in PBP-dependent ABC transporters (PF16296; HMM-score: 8.2)
    Bap31; B-cell receptor-associated protein 31-like (PF05529; HMM-score: 5.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.196
    • Ymax_pos: 11
    • Cmax: 0.13
    • Cmax_pos: 30
    • Smax: 0.584
    • Smax_pos: 2
    • Smean: 0.353
    • D: 0.257
  • predicted transmembrane helices (TMHMM): 8

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFYSFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYVINILDAYRNAERFNRNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPPRHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPVVKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFWAKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYATAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGAVVSLIIGFIVAIVAFIQFRRTSTFKDEGGL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulators: CcpA, MalR
    CcpA (TF) important in Carbon catabolism  RegPrecise
    MalR (TF) important in Maltodextrin utilization, Maltose utilization  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]