From AureoWiki
Jump to: navigation, search
NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0217 [new locus tag: SA_RS01270 ]
  • pan locus tag?: SAUPAN001079000
  • symbol: SA0217
  • pan gene symbol?: hptA
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0217 [new locus tag: SA_RS01270 ]
  • symbol: SA0217
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 257310..258278
  • length: 969
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAATCAAAAATTTATATCTTGCTATTATTTCTCATTTTTTTATCATCATGCGCTAAT
    ACGCGTCACTCTGAATCAGATAAAAATGTATTAACAGTTTATTCTCCGTATCAATCAAAC
    TTGATTCGTCCAATTTTAAATGAATTTGAAAAACAAGAGCATGTCAAAATTGAAATCAAA
    CATGGATCTACTCAAGTACTGCTTTCAAACTTGCATAACGAAGATTTTTCGGAGCGTGGT
    GACGTCTTTATGGGTGGTGTGTTATCAGAAACAATTGATCATCCAGAAGATTTTGTTCCC
    TATCAAGATACATCTGTAACACAGCAATTAGAGGATTATCGCTCGAACAATAAATATGTT
    ACTAGTTTTCTATTAATGCCAACAGTTATAGTAGTGAATTCAGATTTACAAGGAGATATT
    AAGATTCGAGGTTATCAAGATTTATTACAACCTATACTTAAAGGTAAAATTGCGTACTCA
    AATCCAAATACAACAACGACAGGCTATCAACATATGCGCGCTATTTATAGCATGCATCAT
    CGAGTAAGTGATGTGCATCAATTCCAAAACCATGCGATGCAACTGTCAAAGACGTCTAAA
    GTCATTGAAGATGTTGCAAAAGGTAAATATTACGCAGGTCTAAGCTACGAACAAGATGCA
    CGCACATGGAAAAACAAAGGTTATCCTGTATCAATCGTTTATCCAATTGAAGGAACAATG
    TTAAATGTTGATGGTATTGCTTTAGTTAAAAATGCACACCCTCATCCTAAGCGTAAAAAG
    TTAGTGCAATATTTAACAAGCCGCTCAGTACAACAACGATTAGTTGCAGAGTTCGATGCC
    AAGTCAATTCGAAAGGATGTTAGTGAACAAAGCGATCAGTCTATCGAAAATCTTAAGAAC
    ATACCTTTAATACCGAAATCTAAATTACCGGACATTCCACATCATAAATTTTTGGAGATG
    ATTCAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    969

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0217 [new locus tag: SA_RS01270 ]
  • symbol: SA0217
  • description: hypothetical protein
  • length: 322
  • theoretical pI: 8.99606
  • theoretical MW: 36947.9
  • GRAVY: -0.463354

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesputative 2-aminoethylphosphonate ABC transporter, periplasmic 2-aminoethylphosphonate-binding protein (TIGR03261; HMM-score: 88.1)
    2-aminoethylphosphonate ABC transporter, 2-aminoethylphosphonate binding protein (TIGR03227; HMM-score: 40.7)
    MetabolismTransport and binding proteinsOtherABC transporter periplasmic binding protein, thiB subfamily (TIGR01254; HMM-score: 27.2)
  • TheSEED:  
    Iron acquisition and metabolismIron acquisition and metabolism - no subcategoryIron acquisition in Streptococcus Ferric iron ABC transporter, iron-binding protein 
  • PFAM:
    PBP (CL0177) SBP_bac_11; Bacterial extracellular solute-binding protein (PF13531; HMM-score: 74.7)
    SBP_bac_6; Bacterial extracellular solute-binding protein (PF13343; HMM-score: 62.9)
    SBP_bac_8; Bacterial extracellular solute-binding protein (PF13416; HMM-score: 38.7)
    SBP_bac_1; Bacterial extracellular solute-binding protein (PF01547; HMM-score: 23.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: -0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: 5
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide length 25 aa
    • Ymax: 0.476
    • Ymax_pos: 25
    • Cmax: 0.328
    • Cmax_pos: 25
    • Smax: 0.801
    • Smax_pos: 11
    • Smean: 0.701
    • D: 0.564
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKSKIYILLLFLIFLSSCANTRHSESDKNVLTVYSPYQSNLIRPILNEFEKQEHVKIEIKHGSTQVLLSNLHNEDFSERGDVFMGGVLSETIDHPEDFVPYQDTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIRGYQDLLQPILKGKIAYSNPNTTTTGYQHMRAIYSMHHRVSDVHQFQNHAMQLSKTSKVIEDVAKGKYYAGLSYEQDARTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVAEFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]

Joo Youn Park, Jong Wan Kim, Bo Youn Moon, Juyeun Lee, Ye Ji Fortin, Frank W Austin, Soo-Jin Yang, Keun Seok Seo
Characterization of a novel two-component regulatory system, HptRS, the regulator for the hexose phosphate transport system in Staphylococcus aureus.
Infect. Immun.: 2015, 83(4);1620-8
[PubMed:25644013] [WorldCat.org] [DOI] (I p)