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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0223 [new locus tag: SA_RS01310 ]
  • pan locus tag?: SAUPAN001092000
  • symbol: SA0223
  • pan gene symbol?: fadA
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0223 [new locus tag: SA_RS01310 ]
  • symbol: SA0223
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 267239..268423
  • length: 1185
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCAAGAAGCATACATTGTAGCTTATGGGCGTTCAGCCGCAGCGAAAGCAAAGCAAGGC
    GCATTATTCCACGAAAGACCTGATGATGTCGCAGCCAAAGTATTACAAGGCGTATTGAAA
    CGTATTGACGGAAAATTCAATAAGAATATGATTGAAGATGTCATTGTTGGTACGGCTTTT
    CCAGAAGGATTACAAGGACAAAACATTGCACGAACGATTGCATTGCGTGCGGGATTATCT
    GACACGGTACCGGGTCAAACAGTGAATCGCTACTGCTCATCAGGATTACAAACCATCGCG
    ATTGCAGCCAATCAAATTATGGCTGGTCAAGGAGATATACTTGTAGCTGGTGGCGTTGAA
    TTGATGAGTGCCGTACCAATGGGTGGCAACGAGCCCACAAACAATCCAACCTTACAATAT
    GATGATATAGGTGCGTCATATCCTATGGGTTTAACTGCTGAAAATGTAGCATCCCAATTT
    GACGTATCACGCGAAGATCAAGATGCTTATGCTGTCAGAAGTCATCAACGTGCCTATGAC
    GCACAACGTGATGGTCGGTTCAAAGATGAAATTATTCCAATACGGGTAAACTCAGTTGAA
    TATACAAACGCAGGACCAAAAGTACACACAAATATCTTTGACCAAGATGAATTTATACGC
    CCTGACACCACGATGGAGGCATTAGCCAAATTACGTACAGTATTTAAAGCTGACGGCACT
    GTGACTGCGGGAACATCTGCCCCACTTTCTGATGGTGCAGGATTTGTAGTTTTAATGTCT
    GGAGATAAAGTGAAAGAACTAGGCGTGACACCTATTGCACGATTCGTTGGTTATAAGGCA
    GTAGGCGTTGACCCGAAAATTATGGGTATTGGGCCTGCATATGCGATTCCTGAAGTATTG
    TCACTCAGCAATCTATCTGTTGAAGACATTGATTTGATCGAATTGAACGAAGCATTTGCT
    TCTCAAACGATTGCATCTATTAAAGAAGTAGGTCTAGATATATCACGTACGAATGTGAAT
    GGTGGCGCTATTGCTTTAGGTCATCCATTAGGTGCTACAGGGGCAATGTTAACCGCGCGT
    TTACTTAATGAAATGGGTAGACGTCCCGATAGCCGTTACGGCATGGTTACGATGTGTATT
    GGTGTCGGCATGGGTGCAGCTGCTATATTTGAGTATGTGCGTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1185

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0223 [new locus tag: SA_RS01310 ]
  • symbol: SA0223
  • description: hypothetical protein
  • length: 394
  • theoretical pI: 4.89597
  • theoretical MW: 41848.4
  • GRAVY: -0.0269036

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismFatty acid and phospholipid metabolismOtheracetyl-CoA C-acyltransferase (TIGR01930; EC 2.3.1.16; HMM-score: 454.5)
    MetabolismFatty acid and phospholipid metabolismDegradationacetyl-CoA C-acyltransferase FadA (TIGR02445; EC 2.3.1.16; HMM-score: 347.4)
    3-oxoadipyl-CoA thiolase (TIGR02430; EC 2.3.1.174; HMM-score: 343.7)
    MetabolismFatty acid and phospholipid metabolismDegradationacetyl-CoA C-acyltransferase FadI (TIGR02446; EC 2.3.1.16; HMM-score: 198)
    MetabolismFatty acid and phospholipid metabolismBiosynthesisbeta-ketoacyl-acyl-carrier-protein synthase II (TIGR03150; EC 2.3.1.179; HMM-score: 33.5)
  • TheSEED:  
    Biotin biosynthesis 3-ketoacyl-CoA thiolase [fadN-fadA-fadE operon] (EC 2.3.1.16) 
    Isoleucine degradation 3-ketoacyl-CoA thiolase [fadN-fadA-fadE operon] (EC 2.3.1.16) 
    Polyhydroxybutyrate metabolism 3-ketoacyl-CoA thiolase [fadN-fadA-fadE operon] (EC 2.3.1.16) 
    Serine-glyoxylate cycle 3-ketoacyl-CoA thiolase [fadN-fadA-fadE operon] (EC 2.3.1.16) 
  • PFAM:
    Thiolase (CL0046) Thiolase_N; Thiolase, N-terminal domain (PF00108; HMM-score: 240.7)
    Thiolase_C; Thiolase, C-terminal domain (PF02803; HMM-score: 151)
    ketoacyl-synt; Beta-ketoacyl synthase, N-terminal domain (PF00109; HMM-score: 22.6)
    P-loop_NTPase (CL0023) Podovirus_Gp16; Podovirus DNA encapsidation protein (Gp16) (PF05894; HMM-score: 11.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SA2046(rplD)50S ribosomal protein L4  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helix: 1
  • LocateP: Intracellular /TMH start AFTER 60
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: Possibly Sec-
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.112
    • Ymax_pos: 33
    • Cmax: 0.116
    • Cmax_pos: 33
    • Smax: 0.161
    • Smax_pos: 6
    • Smean: 0.097
    • D: 0.106
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDGKFNKNMIEDVIVGTAFPEGLQGQNIARTIALRAGLSDTVPGQTVNRYCSSGLQTIAIAANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQFDVSREDQDAYAVRSHQRAYDAQRDGRFKDEIIPIRVNSVEYTNAGPKVHTNIFDQDEFIRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFVGYKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDISRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEYVR

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CcpA
    CcpA (TF) important in Carbon catabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]