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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0303 [new locus tag: SA_RS01750 ]
  • pan locus tag?: SAUPAN001238000
  • symbol: SA0303
  • pan gene symbol?: nanT
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0303 [new locus tag: SA_RS01750 ]
  • symbol: SA0303
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 358240..359772
  • length: 1533
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAAAGAAGTTGGATTTGGCACACTAAACTGGGTTGCCGTTATCATTTATCTACTAGCT
    ATGTTGTTCATTGGCGTTTATTTTACCAAGCGCGCGAGCCAAAGTACCAATAGTTTCTTT
    ACCGCAAGTGGTCGCTTGCCATCTTGGGTAGTTGGCTTTTCAATTTATGCTACTACGTTA
    AGTGCGATTACATTTATGTCTACACCAGAGAAAGCATTTTTAACAGATTGGTCATATATC
    GCTGGTAACATTGCTATCGTCGCAATTATTCCATTACTTATTTATTTCTATGTCCCTTTC
    TTTAAAAAGTTAAAGGTAACATCTGCATATGAATATTTAGAAGCTAGATTTGGCCCTAGC
    ATACGTGTCATTGGCTCATTATTATTTGTCGTTTACCATTTAGGGCGTGTTGCAATTGTT
    ATCTACTTACCAACATTAGCAATCACATCTGTATCAGACATGAACCCTTATATCGTTGCA
    TCACTCGTAGGTTTACTATGTATTTTATATACATTTTTAGGTGGTTTCGAAGGTGTGGTT
    TGGAGTGATTTCATTCAAGGCGTCATTTTATTAGGCGGCGCTTTAGTTATTATTATTCTA
    GGTGTTATGAACATTAAAGGCGGTTTTGGCACTGTCTTTGCAGATGCGATTGAGCACAAA
    AAATTAATTAGTGCAGACAATTGGAAACTAAATACTGCGGCAGCTGCCATTCCAATTATT
    TTCCTAGGAAATATTTTCAACAACTTGTATCAATACACAGCGAGTCAAGACGTCGTGCAG
    CGTTATCAAGCTTCTGATAGTTTAAAAGAAACAAATAAATCGTTATGGACAAATGGTATC
    CTAGCTTTAATTTCAGCACCCTTATTTTATGGTATGGGTACAATGCTGTATTCATTTTAT
    ACACATGAAGCTGTTTTACCAAAAGGCTTCAATACATCATCTGTAGTGCCATATTTCATT
    TTGACTGAGATGCCACCATTTGTAGCAGGATTACTTATTGCAGCCATTTTCGCCGCTGCA
    CAGTCTACCATTTCATCTAGTTTAAATTCTATATCTGCTTGTATTTCAATCGACATTAAG
    CAACGCTTCTTCGGAAAAGATAGCGAGCGACACGAAGTTAACTTTGCACGTTTCATTATC
    ATCATTGCAGGTATTTTCGGTTTTGGAATGTCACTATACTTAATTGCTTCTAATTCAAAT
    GACTTATGGGATTTATTCTTGTTTGTAACTGGATTATTCGGTGTTCCATTAGCTGGTGTA
    TTTGCAGTTGGTATTTTCACTAAACGTACGAATACATTCGGTGTTATTTGTGGATTAATA
    TTGGGTATCATCTTTGCTTATGTCTATAATGGTGTTGGCAAAGGTAACTCACCTTTCTAT
    GTATCTACCATTTCATTTACAGTTGCTTTTGTCTTTGCTTATATACTTAGCTTCATTGTC
    CCTTCAAAACATAAAAAAGATATAACGGGATTAACAATTTTTGAAAAAGATAAACCATCA
    ACATACATTTCAAAAACGGCTACGAATAAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1533

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0303 [new locus tag: SA_RS01750 ]
  • symbol: SA0303
  • description: hypothetical protein
  • length: 510
  • theoretical pI: 9.43489
  • theoretical MW: 55889.2
  • GRAVY: 0.721765

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundstransporter, solute:sodium symporter (SSS) family (TIGR00813; HMM-score: 222.7)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminessodium/proline symporter (TIGR02121; HMM-score: 99.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPantothenate and coenzyme Asodium/pantothenate symporter (TIGR02119; HMM-score: 72.9)
    MetabolismTransport and binding proteinsOthersodium/pantothenate symporter (TIGR02119; HMM-score: 72.9)
    probable sodium:solute symporter, VC_2705 subfamily (TIGR03648; HMM-score: 58.4)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidscation/acetate symporter ActP (TIGR02711; HMM-score: 30.9)
    MetabolismTransport and binding proteinsCations and iron carrying compoundsmulticomponent Na+:H+ antiporter (TIGR00942; HMM-score: 14.1)
  • TheSEED:  
    Cell Wall and CapsuleCapsular and extracellular polysacchridesSialic Acid Metabolism Predicted sialic acid transporter 
  • PFAM:
    APC (CL0062) SSF; Sodium:solute symporter family (PF00474; HMM-score: 141.7)
    CopD_like (CL0430) UPF0093; Uncharacterised protein family (UPF0093) (PF03653; HMM-score: 12.7)
    no clan definedLRR19-TM; Leucine-rich repeat family 19 TM domain (PF15176; HMM-score: 8.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.203
    • Ymax_pos: 35
    • Cmax: 0.174
    • Cmax_pos: 35
    • Smax: 0.371
    • Smax_pos: 31
    • Smean: 0.142
    • D: 0.179
  • predicted transmembrane helices (TMHMM): 13

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKEVGFGTLNWVAVIIYLLAMLFIGVYFTKRASQSTNSFFTASGRLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFKKLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVASLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVMNIKGGFGTVFADAIEHKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLWTNGILALISAPLFYGMGTMLYSFYTHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIAAIFAAAQSTISSSLNSISACISIDIKQRFFGKDSERHEVNFARFIIIIAGIFGFGMSLYLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYNGVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTATNK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulators: SA0306 (NanR*), CcpA
    NanR* (TF) important in N-acetylglucosamine utilization, Sialic acid utilizationRegPrecise
    CcpA (TF) important in Carbon catabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]

Michael E Olson, Jessica M King, Timothy L Yahr, Alexander R Horswill
Sialic acid catabolism in Staphylococcus aureus.
J. Bacteriol.: 2013, 195(8);1779-88
[PubMed:23396916] [WorldCat.org] [DOI] (I p)